Method for quickly and quantitatively measuring urine iodine

A technology for quantitative determination of urinary iodine, which is applied in the direction of analyzing materials through chemical reactions, can solve problems such as environmental pollution, large amount of reagents, and inability to measure samples in batches, so as to reduce the amount of determination reagents, reduce detection costs, and simplify The effect of the procedure

Inactive Publication Date: 2012-06-20
WENZHOU MEDICAL UNIV
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  • Abstract
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Problems solved by technology

[0005] The standard method has been widely used in the determination of urinary iodine due to its high sensitivity. However, in this method, the digestion and photocatalytic reaction process of the sample needs to be carried out one by one in the test tube, which not only makes it impossible to determine the samples in batches, but also reduces the amount of reagents. Large, especially the large amount of arsenous acid, resulting in certain environmental pollution

Method used

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  • Method for quickly and quantitatively measuring urine iodine
  • Method for quickly and quantitatively measuring urine iodine
  • Method for quickly and quantitatively measuring urine iodine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1 standard curve and sample test

[0018] The method for quantitatively detecting iodine content in urine of the present invention comprises steps:

[0019] 1. Use a 12-channel pipette gun to absorb and simultaneously absorb 50 μL of 7 parts of iodine standard series solutions of different concentrations and add them to the first row of microwells of a 96-microwell plate, and then use a pipeline gun to simultaneously absorb 50 μL of 12 urine samples and add them to the microwells of the microwell plate In the second row of microwells, draw 50 μL of another four batches of urine samples (12 copies in each batch) and place them in the third, fourth, fifth, and sixth rows of microwells of the microwell plate, and then use a row gun to inject samples into the microwells. Add 100 µL of ammonium persulfate.

[0020] 2. Seal the microwell plate and place it in a constant temperature drying oven at 100°C for 60 minutes to digest.

[0021] 3. After digestion, cool t...

Embodiment 2

[0027] Embodiment 2 recovery rate

[0028] Add 50 μg / L, 100 μg / L, and 150 μg / L potassium iodate standard solutions to the urine specimens whose urinary iodine concentration has been measured to be 102 μg / L, and carry out the recovery experiment according to the experimental method, and the recovery rates are respectively 93.3%. , 105.9% and 96.6%, the average recovery rate was 98.6%.

Embodiment 3

[0029] Example 3 Precision

[0030] Intra-assay precision: urine samples with high iodine concentration (256 μg / L), urine samples with medium iodine concentration (152 μg / L), and urine samples with low iodine concentration (49 μg / L) were taken respectively, each urine sample Twelve specimens were taken in parallel and measured in the same batch in a microwell plate. The average coefficient of variation (CV) of high, medium and low samples were 3.35%, 2.32% and 5.61%, respectively. Inter-assay precision: the urine samples with concentration (152 μg / L) were taken for continuous measurement for 3 days, 3 times a day, and the inter-assay average coefficient of variation was 4.50%.

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Abstract

The invention relates to a method for quickly and quantitatively measuring urine iodine. The method comprises the steps of: adopting polypropylene microwell plates with good heat resistance and chemical inertness as a digestion container and catalytic reaction container; preventing cross contamination of samples and water vapor leakage from the microwell plate by using a self-made sealing plate; and simultaneously measuring light absorbance of a plurality of samples at the same time by using an ELIASA reader. The method realizes batch measurement of micro samples. According to the method provided by the invention, the usage amount of reagents is greatly reduced, the measuring time is greatly shortened, and the workload in a measuring process is reduced, so that the method is suitable for measuring a large batch of samples. The method of the invention requires only simple instrument, and is low in analysis cost. The analysis efficiency is a little higher than that of an automatic analysis method.

Description

technical field [0001] The invention belongs to detection reagents, in particular to a detection method for determining iodine content in urine. Background technique [0002] Iodine deficiency and iodine excess, as diseases that seriously endanger human health, have attracted widespread attention, so it is particularly important to accurately evaluate the iodine nutritional status of the population. Except for the iodine ingested by the thyroid gland (about 50-75 μg / L), the rest of the iodine ingested by the human body is excreted from the body, and 80%-85% of it is excreted through urine. best indicator of the situation. [0003] At present, the health industry standard method for the determination of urinary iodine in my country is ammonium persulfate digestion-arsenic cerium catalytic spectrophotometry. This method uses the digestion of samples one by one in a test tube, the amount of reagents is large, the operation process is cumbersome, and the reaction time is strict...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/75
Inventor 吕建新曹建明楼永良楼晓明丁钢强赵长容郑美琴刘佳明
Owner WENZHOU MEDICAL UNIV
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