Urine iodine determination kit and applications thereof

A kit and urine technology, applied in the field of in vitro diagnostic reagents, can solve the problems of complex adsorbents and oxidants, high cost, misjudgment, etc., and achieve the effects of low price, stable color interpretation, and cost reduction

Inactive Publication Date: 2015-10-28
杨先锋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the adsorbents and oxidants used in this method are relatively complex and costly, especially when interpreting the results, because the reaction has not reached the end or is in an equilibrium s...

Method used

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  • Urine iodine determination kit and applications thereof
  • Urine iodine determination kit and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0026] The urine iodine determination kit of the present invention is made up of adsorbent, diluent, chromogenic agent, oxidizing agent, stop solution, standard and color standard color plate; Wherein, described adsorbent is silica gel, and its dosage is urine 1 / 6 to 1 / 8 of the sample weight; the diluent is composed of pH4 acetate buffer and 5% MgCl 2 And the solution that the sodium azide that mass percentage concentration is 2% makes; Described developer is the tetramethylbenzidine solution that mass percentage concentration is 4.5%; Described oxygenant is the peracetic acid that mass percentage concentration is 9% solution; the stop solution is a sodium azide solution with a mass percent concentration of 10%; the standard substance is a potassium iodide urine mixture with an iodine concentration of 50 to 350ug / L; The potassium iodide urine mixture of 50-350ug / L is subjected to the detection reaction described in this kit according to the set iodine concentration gradient, a...

Embodiment 2

[0028] The urine iodine determination kit of the present invention is made up of adsorbent, diluent, chromogenic agent, oxidizing agent, stop solution, standard substance and color standard color plate; Wherein, described adsorbent is molecular sieve, and its dosage is urine 3 to 1 / 5 of the sample weight; the diluent is a solution made of citric acid buffer solution with pH4.5, 3% NaBr and 5% sodium azide in mass percentage concentration; Described chromogenic agent is the tetramethylbenzidine solution that mass percentage concentration is 1%; Described oxidant is the sodium hypochlorite solution that mass percentage concentration is 10%; Described stop liquid is the sodium fluoride solution that mass percentage concentration is 2%; Described standard product is the potassium iodate urine mixed solution that iodine concentration is 50~350ug / L; The iodine concentration gradient of the iodine concentration gradient is respectively carried out with the detection reaction describe...

Embodiment 3

[0030] The urine iodine determination kit of the present invention is made up of adsorbent, diluent, chromogenic agent, oxidizing agent, stop solution, standard substance and color scale color guide; Wherein, described adsorbent is aluminum oxide, and its consumption is 1 / 20 of the urine sample weight; the diluent is a solution made of acetic acid buffer solution of pH 5.5, 8% NaCL and 0.2% thimerosal in a mass percentage concentration; The coloring agent is a tetramethylbenzidine solution with a mass percent concentration of 10%; the oxidizing agent is a permanganic acid solution with a mass percent concentration of 5 percent; the stop solution is a sulfuric acid solution with a mass percent concentration of 1 percent; The standard product is a potassium iodide urine mixture with an iodine concentration of 50-350ug / L; the color standard color guide is made of a potassium iodide-urine mixture with an iodine concentration of 50-350ug / L according to the set iodine concentration g...

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Abstract

The present invention discloses a urine iodine determination kit. According to the kit, based on the adsorbent, the dilution liquid, the coloring agent and the standard substance of the existing kit, a hydrogen peroxide solution, peroxyacetic acid solution, sodium hypochlorite solution or potassium permanganate solution with a mass percentage concentration of 5-12% is selected as an oxidizing agent and a special terminating liquid is arranged, and the terminating liquid comprises a sulfuric acid solution, hydrochloric acid solution, citric acid solution, sodium fluoride solution, sodium azide solution or potassium iodate solution with a mass percentage concentration of 1-20% or a combination comprising any solutions selected from the sulfuric acid solution, the hydrochloric acid solution, the citric acid solution, the sodium fluoride solution, the sodium azide solution and the potassium iodate solution according to any mass percentage ratio. According to the present invention, the single adsorbent and the single oxidizing agent are used so as to significantly reduce the cost, and the terminating liquid is used to rapidly terminate the reaction, such that it is ensured that the color is not significantly changed within a certain time, and the stable detection result coloring and the accurate detection result interpretation are achieved.

Description

technical field [0001] The invention relates to a urine test kit and its application, in particular to a urine iodine determination kit and its application, and belongs to the field of in vitro diagnostic reagents. Background technique [0002] Iodine is one of the essential trace elements for the human body, and its biological function is mainly reflected by the thyroxine synthesized in the thyroid gland. The human body generally obtains iodine through air, drinking water and food. Usually, about 90% of the iodine ingested by the human body is excreted through urine, and 5-10% is excreted through feces. Based on this, the determination of urinary iodine index is closely related to clinical evaluation of human iodine nutritional status. [0003] Urinary iodine analysis methods include spectrophotometry, gas chromatography, electrochemical method, ion chromatography, etc., but some of these methods are complicated in the analysis process, and some of the equipment are expen...

Claims

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Application Information

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IPC IPC(8): G01N21/78G01N21/31
Inventor 杨先锋朱晓蕾
Owner 杨先锋
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