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Ratio type copper ion fluorescence sensing system, double-tube detection bottle and application of double-tube detection bottle

A technology of copper ion detection and fluorescence sensing, which is applied in the direction of fluorescence/phosphorescence, luminescent materials, measuring devices, etc., can solve the problems of unfavorable real-time visual detection, high requirements for operators, and long detection time, so as to achieve rapid visual detection, Good selectivity and low detection line

Active Publication Date: 2022-04-22
ZHENGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the deficiencies of the existing copper ion detection methods, such as long detection time, expensive equipment, high requirements for operators, and unfavorable real-time visual detection on site, the invention discloses a ratio-type copper ion fluorescence sensing system and a dual-tube detection system. bottle and its application, the sensing system of the present invention can realize rapid visual detection of copper ions on the one hand, on the other hand, it is less affected by environmental factors when performing quantitative detection, with good repeatability and high accuracy

Method used

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  • Ratio type copper ion fluorescence sensing system, double-tube detection bottle and application of double-tube detection bottle
  • Ratio type copper ion fluorescence sensing system, double-tube detection bottle and application of double-tube detection bottle
  • Ratio type copper ion fluorescence sensing system, double-tube detection bottle and application of double-tube detection bottle

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Experimental program
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Effect test

Embodiment 1

[0044] The preparation of probe 1 comprises the following steps:

[0045] S1. 2.96g of 4-(diethylamino) salicylaldehyde (15mmol) and 3.81g diethyl malonate (23.5mmol) are dissolved in 105mL toluene and acetonitrile by volume ratio 1: 2 in the mixed solvent of composition, And add 4.5ml of piperidine as a catalyst, heat to reflux at 90°C for 10h, and remove the solvent by rotary evaporation to obtain ethyl 7-(diethylamino)coumarin-3-carboxylate.

[0046] S2. 2.17g of 7-(diethylamino)coumarin-3-ethyl carboxylate (7.5mmol) and 1.82mL of hydrazine hydrate (30mmol, a mass fraction of 80% hydrazine hydrate solution) were added to 20mL of ethanol solution , cooled in an ice-water bath for 15 minutes, and stirred at room temperature for 12 minutes. The reacted crude product was purified by column chromatography to obtain 7-(diethylamino)coumarin-3-carboxylhydrazide.

[0047] S3. Dissolve 0.6056g of 7-(diethylamino)coumarin-3-carboxylhydrazide (2.2mmol) and 0.1352g of pyridoxal (0.8mm...

Embodiment 2

[0051] The application of the ratiometric copper ion fluorescence sensing system in the detection of copper ions, the detection steps are as follows:

[0052] (1) Dissolving the probe 1 prepared in Example 1 in DMSO to prepare a stock solution 1, the concentration of the stock solution 1 is 2 mM;

[0053] (2) Probe 2 was dissolved in DMSO to prepare stock solution 2. The concentration of the stock solution 2 was 0.2 mM. The CAS number of probe 2 in this experiment was 20571-42-0, which was purchased from the official website of Bailingwei;

[0054] (3) Prepare a PBS solution with a concentration of 10 mM and a pH of 7.4, and prepare a 2 mM copper ion solution with water as a solvent.

[0055] (4) Add 10 μL of stock solution 1 and 10 μL of stock solution 2 to different volumes of PBS solutions, and then add different volumes of copper ion solutions, the final volume is 2ml, so that the final concentration of probe 1 is 10 μM, probe 2 The final concentration of copper ions is 1...

Embodiment 3

[0061] The selectivity of the ratiometric copper ion fluorescent sensing system to copper ions:

[0062] Prepare 2mM copper ion solution and 10mM other interfering ion solutions with water as solvent, interfering ion solutions include: sodium ion solution (Na + ), potassium ion solution (K + ), calcium ion solution (Ca 2+ ), barium ion solution (Ba 2+ ), ferrous ion solution (Fe 2+ ), zinc ion solution (Zn 2+ ), divalent cadmium ion solution (Cd 2+ ), magnesium ion solution (Mg 2+ ), ferric ion solution (Fe 3 + ), Mercury ion solution (Hg 2+ ), divalent lead ion solution (Pb 2+ ), divalent manganese ion solution (Mn 2+ ), aluminum ion solution (Al 3+ );

[0063] Add 10 μL of stock solution 1 and 10 μL of stock solution 2 to PBS solution (10 mM, pH=7.4), and then add 10 μL of copper ion solution and 20 μL of different interfering ion solutions, the total volume of each solution is 2 ml, and the final volume of probe 1 The concentration is 10 μM, the final concentra...

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Abstract

The invention discloses a ratio type copper ion fluorescence sensing system, a double-tube detection bottle and application of the double-tube detection bottle, and mainly aims to solve the problems that an existing copper ion detection method is long in detection time, expensive in equipment, not beneficial to instant visual detection and the like. The ratio type copper ion fluorescence sensing system comprises a probe 1 and a probe 2, the molecular formula of the probe 1 is C22H24N4O5, and the molecular formula of the probe 2 is C22H24N4O5. The probe 2 is 7-(diethylamino) coumarin, the ratio type copper ion fluorescence sensing system can be subjected to a rapid recognition reaction with copper ions, colorimetric and fluorescence dual-mode signal output can be realized for recognition of the copper ions, the selectivity is good, the detection line is low, and qualitative and quantitative detection can be performed on the copper ions; a portable double-tube detection bottle is further designed based on the recognition reaction, rapid and visual detection of the copper ions can be achieved, accuracy is high, probe loss is small, operation is easy, detection can be achieved after extrusion, and the detection bottle is not limited by detection conditions and professional knowledge of detection personnel.

Description

technical field [0001] The invention relates to the field of rapid detection of copper ions, in particular to a ratio-type copper ion fluorescence sensing system, a double-tube detection bottle and applications thereof. [0002] technical background [0003] Copper ions are closely related to human health. As catalytic cofactors of proteins and various enzymes, they play a vital role in various physiological processes. Normal levels of copper ions are essential to health, but excessive copper ions It will lead to cancer, neurodegenerative diseases, Alzheimer's disease, Parkinson's disease, gastrointestinal disorders and many other diseases. Therefore, it is crucial to develop a simple, rapid, and visual method for the detection of copper ions. [0004] At present, the determination methods of copper ion content mainly include: inductively coupled plasma emission spectrometry, inductively coupled plasma mass spectrometry, atomic absorption spectrometry and other methods. How...

Claims

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Application Information

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IPC IPC(8): C09K11/06C07D405/12G01N21/01G01N21/64G01N21/78
CPCC09K11/06C07D405/12G01N21/6428G01N21/643G01N21/6447G01N21/78G01N21/01C09K2211/1029C09K2211/1088Y02A50/30
Inventor 杨冉代秀秀孙远强屈凌波李朝辉
Owner ZHENGZHOU UNIV
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