47results about How to "Detection line low" patented technology

The invention discloses a preparation method and a use of a functional graphene-loaded palladium nanoparticle composite material. The preparation method comprises the following steps of carrying out ultrasonic dispersion of graphene in water to obtain a graphene suspension liquid, adding a pyrenetetrasulfonic acid sodium salt aqueous solution into the graphene suspension liquid, carrying out heating stirring for 8-15h, carrying out centrifugal filtration to obtain functional graphene, dispersing the functional graphene in water to obtain a functional graphene suspension liquid, adding a palladium chloride solution into the functional graphene suspension liquid, adding a sodium borohydride solution into the mixed solution, carrying out heating stirring for 8-15h, and carrying out centrifugal filtration to obtain the functional graphene-loaded palladium nanoparticle composite material. Through functionalization of graphene by the pyrenetetrasulfonic acid sodium salt, dispersibility and stability of graphene and palladium nanoparticles are improved. A glassy carbon electrode modified by the functional graphene-loaded palladium nanoparticle composite material has a good nitroaromatic compound catalysis performance, a low nitroaromatic compound detection limit, a wide nitroaromatic compound detection linear range, high nitrobenzene detection sensitivity, a nitrobenzene detection linear range of 170-8ppb, and a nitrobenzene detection limit of 0.62ppb.

The invention relates to the technical field of immunochromatography detection and particularly discloses a kit for testing of the stomach function and a preparation method of the kit. The kit comprises a plastic board, a glass cellulose membrane, a nitrocellulose membrane, a sample pad and a water absorbent pad, wherein the glass cellulose membrane is coated with a quantum-dot-labeled mixture of pepsinogen I antibodies, pepsinogen II antibodies and helicobacter pylori urease antigens; a detection line 1, a detection line 2, a detection line 3 and a control line are arranged on the nitrocellulose membrane, the detection line 1 is coated with pepsinogen I secondary antibodies, the detection line 2 is coated with pepsinogen II secondary antibodies, the detection line 3 is coated with helicobacter pylori urease antigens, and the control line is coated with goat-anti-mouse lgG. According to the kit, fast and sensitive combined detection of pepsinogen I, pepsinogen II and helicobacter pylori urease antibodies is realized with the fluorescence immunochromatography method by means of the double-antibody/antigen sandwich principle in combination with the fluorescence characteristic of quantum dots.

The invention relates to the technical field of immunochromatographic assay, and particularly discloses a kit for myocardial function assay and a preparation method thereof. The kit comprises a plastic plate, a glass cellulose membrane, a nitrocellulose membrane, a sample pad and a water absorbing pad, and the glass cellulose membrane is coated with a mixture of a troponin antibody, a myoglobin antibody and a creatine jubase antibody which are labeled with quantum dots; the nitrocellulose membrane is provided with an assay line 1, an assay line 2, an assay line 3 and a control line, the assay line 1 is coated with a troponin secondary antibody, the assay line 2 is coated with a myoglobin secondary antibody, the assay line 3 is coated with a creatine jubase secondary antibody, and the control line is coated with goat anti-mouse IgG. According to the kit, a double antibody and antigen sandwich principle is combined with the fluorescence characteristic of the quantum dots, and rapid and sensitive combined assay to troponin, myoglobin and creatine jubase is achieved by means of the fluorescent immunochromatographic method.

The invention discloses a method for photoelectrochemically detecting an organophosphorus pesticide based on a BiOBr/Bi2S3 semiconductor heterojunction generated by biological induction. The method comprises the following steps: mixing malathion with avidin-modified magnetic beads, biotin-modified auxiliary DNA and a malathion aptamer, incubating, removing part of the malathion aptamer by using malathion to recover the single strand of the auxiliary DNA, triggering by using the auxiliary DNA to form G-rich dsDNA, and embedding the dsDNA into Hemin and MnTMPyP to catalyze a Na2S2O3 and H2O2 mixed solution to generate H2S. H2S reacts with BiOBr modified on an electrode to generate a BiOBr/Bi2S3 compound, so that the photocurrent is greatly increased, the change of the photocurrent and the concentration of a target object are in a linear relationship in a certain range, HCR signal amplification is designed on the basis of specific recognition of malathion and an aptamer thereof, biocatalysis is utilized to induce a BiOBr/Bi2S3 semiconductor heterojunction to amplify a signal, therefore, high-sensitivity and high-selectivity photoelectric method detection of malathion is realized.

The invention relates to the technical field of immunochromatographic assay, and particularly discloses an immunochromatographic kit for joint detection of four enteroviruses and a preparation method for the immunochromatographic kit. The kit comprises a first test strip and a second test strip, wherein each of the first and second test strips comprises a plastic plate, a fiberglass membrane and a cellulose nitrate membrane; a detection line 1, a detection line 2 and a control line are arranged on the cellulose nitrate membrane; the fiberglass membrane of the first test strip is coated with a quantum dot-labeled adenovirus antibody and a quantum dot-labeled rotavims antibody; the detection line 1 of the first test strip is coated with an adenovirus antigen, and the detection line 2 is coated with a rotavims antigen; the fiberglass membrane of the second test strip is coated with a quantum dot-labeled astrovirus antibody and a quantum dot-labeled norovirus antibody; the detection line 1 of the second test strip is coated with an astrovirus antigen, and the detection line 2 is coated with a norovirus antigen. The kit can be used for implementing rapid and sensitive joint detection of the enteroviruses.

The invention discloses an ultraviolet lamp ionizing device taking photoelectrons as an electron source. The ultraviolet lamp ionizing device comprises an ionizing cavity, an ultraviolet lamp, a repulsion electrode, a focusing ring, an orifice plate, a capillary sample and a lens system. In the invention, the ultraviolet lamp generates photoelectrons on the metal electrode, and then the photoelectrons are accelerated to be collided with molecules of the sample by utilizing an electric field to realize electron ionizing. The ultraviolet lamp ionizing device has the characteristics of wide range of samples to be tested, oxidation resistance, long service life, wide working voltage range and the like so as to have broad application prospect on the aspects of process monitoring and pollutant real-time on-line analysis.

The invention belongs to the technical field of nano materials and particularly relates to aminated mesoporous silica-glucose-manganese dioxide nanocomposite and a preparation method and application thereof. The nanocomposite is prepared from manganese dioxide nanosheet and aminated mesoporous SiO2 nanosphere containing glucose, wherein the surface of the aminated mesoporous SiO2 nanosphere is coated by the manganese dioxide nanosheet, the particle size of the aminated mesoporous SiO2 nanosphere is 40 to 60nm, and the hole diameter is 2 to 3nm. The preparation method of the aminated mesoporous silica-glucose-manganese dioxide nanocomposite comprises the steps of preparing aminated mesoporous SiO2 and MnO2 nanosheet, then dispersing the aminated mesoporous SiO2 into a glucose water solution and finally connecting the MnO2 nanosheet to the surface of aminated MSN. According to the aminated mesoporous silica-glucose-manganese dioxide nanocomposite and the preparation method and application thereof, PGM is utilized to detect; thus, the content of GSH in a sample of the aminated mesoporous silica-glucose-manganese dioxide nanocomposite can be quantitatively detected, a detection line is low, and flexibility is high.

The invention provides luciferase substrates, and a preparation method and application thereof, belonging to the technical field of luciferase substrate preparation. The luciferase substrates have a structural formula as described in the specification. In the structural formula, R is hydrogen or deuterium. The luciferase substrates provided by the invention have the advantages of good selectivity,high sensitivity, low detection line, good biocompatibility and the like; and further studies prove that d2-cycluc is higher in bioluminescence intensity and longer in bioluminescence time in vitro,in cells and in vivo compared with cycluc, and the two luciferase substrates have good concentration dependence. The preparation method for the luciferase substrates in the inventions simple in process, high in operability and relatively low in cost, and thus has a good practical application value.

The invention discloses a single-pulse detection method for an immune reaction with metal ion labeling. Single-pulse signal detection is achieved by utilizing inductively coupled plasma or microwave plasma mass spectrometry analysis technology, the immune reaction is labeled by employing metal nanoparticles, and pulse signals of metal ions of the labeled immune reaction are further detected by utilizing the inductively coupled plasma or microwave plasma mass spectrometry analysis technology, thereby achieving a purpose of indirectly detecting a to-be-detected object.

The invention discloses an ion-selective electrode flow injection analyser. Ports of a three-way valve are connected with an ion selective electrode (ISE), a No.1 channel of a six-way valve and a peristaltic pump; the other end of the peristaltic pump A is connected with a total ionic strength adjustment buffer (TISAB) bottle; two ends of a peristaltic pump B are connected with a carrier liquid bottle and a No.6 channel; two ends of a peristaltic pump C are connected with a waste liquor bottle and a No.4 channel; a sample liquid bottle is connected with a No.3 channel; two ends of a separation column are connected with a No.2 channel and a No.5 channel; the bottom end of the ISE is provided with an ISE electrode membrane, a reflux tube is arranged below the ISE electrode membrane, and a gap between the reflux tube and the ISE electrode membrane is 0.5mm; a reference electrode and the ISE electrode are connected through a gravity separator consisting of an A-B channel and a C-D channel; inclination angles of the A-B channel and the C-D channel are 35 degrees and 45 degrees; and channels on two sides of the reflux tube are connected with the ends B and D of the gravity separator. The ion-selective electrode flow injection analyser has the advantages that: experimental reagents are saved, a detection line can be reduced, and a phenomenon that the detection limit is improved due to the dissolution of a salt type ISE membrane is inhibited.

The invention provides a mass spectrum system and a measurement method thereof. The mass spectrum system comprises an ion source subsystem, an ion accelerator subsystem, a high-energy analyzer subsystem and a particle identification and detector subsystem which are connected in sequence. The ion source subsystem comprises a sample injector component and a super-strong ionization ion source component connected with the sample injector component; the high-energy analyzer subsystem comprises an analyzer component connected with the ion accelerator subsystem and a beam measurement component connected with the analyzer component; and the detector subsystem comprises a thin film connected with the beam measurement component and a detector connected with the thin film. Interference of molecular ions is eliminated by adopting a super-strong ionization technology; the size of the atomic number Z can be detected by adopting a particle identification technology, so that the same amount of ectopic ions and the information of ions with different mass numbers but the same M/q, namely ZM/q information, are obtained, and finally, the real mass spectrum measurement is realized.