Preparation method of magnetic nano mimic enzyme, prepared mimic enzyme and application of mimic enzyme in detection of mercury ions

A magnetic nanometer and enzyme-mimicking technology, applied in the field of mercury ion detection, to achieve the effects of reducing detection costs, simplifying the washing process, and mild reaction conditions

Pending Publication Date: 2022-04-08
中科合肥智慧农业协同创新研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The patent with the publication number CN103341360A discloses a nanomaterial imitating enzyme and its application in the detection of mercury ions. After mixing mercury ions and silver nanomaterials, it can catalyze and oxidize various horseradish peroxidases in the presence of oxygen in the air. Characteristic substrate, but its detection line is only 20nM

Method used

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  • Preparation method of magnetic nano mimic enzyme, prepared mimic enzyme and application of mimic enzyme in detection of mercury ions
  • Preparation method of magnetic nano mimic enzyme, prepared mimic enzyme and application of mimic enzyme in detection of mercury ions
  • Preparation method of magnetic nano mimic enzyme, prepared mimic enzyme and application of mimic enzyme in detection of mercury ions

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Embodiment 1

[0049] The preparation method of magnetic nano-mimetic enzyme specifically comprises the following steps:

[0050] (1) 2mmol cobalt chloride hydrate (CoCl 2 ·6H 2 O), 4mmol hydrated ferric chloride (FeCl 3 ·6H 2 (2) and 16mmol sodium hydroxide were dissolved in 30mL ethylene glycol, after stirring and dissolving, ultrasonic 30 minutes formed a yellow solution;

[0051] (2) Put the solution obtained in step (1) into a polytetrafluoroethylene container, put it in an oven, react at 180°C for 8 hours, cool it down to room temperature naturally, and take out the black suspension;

[0052] (3) Under the action of the permanent magnet, the suspension in the step (2) is washed three times with deionized water and ethanol respectively, and after vacuum drying, the magnetic nano-mimetic enzyme is obtained.

Embodiment 2- Embodiment 6

[0054] The difference between Example 2-Example 6 is that the ratio of raw materials, reaction temperature and reaction time are different, and the specific differences are shown in Table 1.

[0055] Table 1 is each raw material consumption and reaction condition of embodiment 2-embodiment 6

[0056]

Embodiment 7

[0058] Adopt the magnetic nano-mimetic enzyme in embodiment 2 to detect mercury ion (Hg 2+ ), the detection schematic diagram is as follows figure 1 As shown, the specific steps are as follows:

[0059] (1) Prepare 1 mg / mL cobalt ferrite nanozyme suspension (solvent is water), prepare HAc-NaAc buffer solutions (0.1M) with different pH values, prepare different concentrations of TMB solutions, H 2 o 2 solution and mercury ion standard solution.

[0060] (2) Add 100μL 1mg / mL cobalt ferrite magnetic nanoparticles, 100μL 0.7mM TMB aqueous solution, 100μL 16mM hydrogen peroxide aqueous solution to 400μL HAc-NaAc (pH=4.0, 0.1M), and react for 30 minutes to obtain a blue color oxTMB solution.

[0061] (3) Mix 200 μL of 20 μM cysteine ​​aqueous solution with 200 μL of different concentrations (10 -9 -5*10 -6 M) mixed with the mercury ion solution and incubated at 30°C for 30 minutes.

[0062] (4) Mix the solutions in steps (2) and (3) and incubate at room temperature for 10 min...

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Abstract

The invention discloses a preparation method of a magnetic nano mimic enzyme, and relates to the technical field of mercury ion detection, and the preparation method comprises the following steps: (1) dissolving cobalt chloride hexahydrate, ferric trichloride hexahydrate and sodium hydroxide in ethylene glycol, stirring and dissolving, and carrying out ultrasonic treatment to form a solution, the molar ratio of cobalt chloride hexahydrate to ferric trichloride hexahydrate to sodium hydroxide being 1: 2: 16; (2) carrying out hydrothermal reaction on the solution in the step (1), and cooling to room temperature after the reaction; and (3) washing the reaction product in the step (2) under the attraction of a permanent magnet, and then drying. The invention also provides the magnetic nano mimic enzyme prepared by the method and application of the magnetic nano mimic enzyme in mercury ion detection. The preparation method has the beneficial effects that only the precursor and the reducing agent are adopted, the magnetic nano mimic enzyme is synthesized through a simple hydrothermal method, and the reaction condition is mild; the strong magnetic property of the magnetic nano mimic enzyme is fully utilized, the washing process is simplified, and the synthesized magnetic nano material can be recycled and reused.

Description

technical field [0001] The invention relates to the technical field of mercury ion detection, in particular to a preparation method of a magnetic nano-mimetic enzyme, the prepared mimetic enzyme and its application in detecting mercury ions. Background technique [0002] In recent years, with the improvement of people's living standards, the emphasis on food safety has also been increasing. As a heavy metal ion that is highly harmful to the human body and the environment, mercury ions not only cannot be degraded by microorganisms, but also easily accumulate in the human body, endangering human health. Regulatory agencies such as the Environmental Protection Agency (EPA) and the World Health Organization (WHO) have set the maximum allowable limits for mercury in drinking water as 10nM and 30nM respectively, so it is important to establish a method that can quickly detect the content of mercury ions in water stand up. [0003] At present, the traditional methods for detectin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/75G01N21/29G01N21/33G01N21/78
Inventor 黄青刘超王儒敬余立祥
Owner 中科合肥智慧农业协同创新研究院
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