Immunoassay method based on iron (II) and phenanthroline system and glucose-encapsulated liposome

An o-phenanthroline and immunoassay technology, which is used in material analysis by observing the effect on chemical indicators, analysis by chemical reaction of materials, and material inspection products, etc., to achieve convenient use, low price, and improved sensitivity. Effect

Inactive Publication Date: 2018-04-27
FUZHOU UNIV
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Despite some advances in the field of colorimetric immunoreaction, there is still a need to develop some new signal conversion strategies for colorimetric methods

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Immunoassay method based on iron (II) and phenanthroline system and glucose-encapsulated liposome
  • Immunoassay method based on iron (II) and phenanthroline system and glucose-encapsulated liposome
  • Immunoassay method based on iron (II) and phenanthroline system and glucose-encapsulated liposome

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1. Preparation of Glucose-Loaded Liposomes (GLL)

[0035] First, hydrogenated soybean lecithin, cholesterol and phosphoethanolamine (molar ratio: 50:10:1) were added to a mixed solution of chloroform and ethanol (5.0 mL, 3:1, v / v) to dissolve. After sonicating for 5 minutes at room temperature, the mixture was kept under the protection of nitrogen for 45 o C Rotary evaporate until a well mixed film forms. Next, an excess of dextrose solution (5 mM, 10 mL) was injected by syringe, at 45 o Sonicate for 5 minutes at C. In order to obtain a suspension with uniform particle size distribution, the suspension was filtered with a 0.4 μm polycarbonate membrane several times. To remove free glucose molecules, the dispersion was dialyzed against deionized water for 2 h in a dialysis cassette (MWCO, 12–14 kDa). The finally obtained glucose-loaded liposomes (GLL) were redispersed in phosphate buffered saline (1.0 mL, pH 7.4) and stored at a temperature of 4 o C space is left fo...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
particle diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to view more

Abstract

The invention discloses an immunoassay method based on an iron (II) and phenanthroline system and glucose-encapsulated liposome. According to the method, glucose-loaded liposome with a high-efficiencyloading capacity and glucose oxidase with a high-efficiency catalytic property are used for signal amplification, and the ferrous ion and phenanthroline system is used as a visual visible chromogenicgroup. The method can be used for high-resolution colorimetric semiquantitative detection of the concentration of streptomycin by a smart mobile phone; along with the increase of the concentration ofstreptomycin, the color of a detection solution is changed from bright yellow to reddish orange, and quantitative detection is performed by an ultraviolet and visible spectrophotometer. The glucose-loaded liposome preparation process is simple and low in cost; the colorimetric detection method of the ferrous ion and phenanthroline color development system is simple and convenient, fast in color development speed and high in sensitivity, and a new sensitive and stable-property visual detection method is provided for food safety detection.

Description

technical field [0001] Immunoassay method based on iron (II) and o-phenanthroline system and liposome-encapsulated glucose, nanomaterials and bioanalysis technology field. Background technique [0002] The overuse of antibiotics in animal husbandry and agriculture has resulted in the presence of antibiotic residues in a large amount of food and is a potential threat to human health. The importance of safe detection of antibiotic residues in food has greatly driven the need for simple, sensitive and low-cost bioanalytical methods. So far, various immunological methods and strategies based on different signal generation principles have been reported, such as: electrochemical-based methods, electrochemiluminescence-based methods, chemiluminescence-based methods, and colorimetric methods. In particular, the colorimetric immunoassay has been widely used due to its advantages of simplicity, convenience, practicality, low cost and visual detection with the naked eye. Although the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/78G01N21/31
CPCG01N21/31G01N21/78G01N33/68
Inventor 唐点平任蓉蓉蔡国能余镇重
Owner FUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap