Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Alkaline Phosphatase Analysis Method Based on Gold Nanorod Long Silver Shell

A technology of gold nanorods and analytical methods, which is applied in the fields of nanomaterials and biological analysis, can solve the problems of low visual resolution of gold nanoparticles and the inability to carry out high-resolution type, and achieve a colorimetric detection method that is simple, cheap, and sensitive performance effect

Inactive Publication Date: 2016-10-05
FUZHOU UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The combination of nano-gold-induced silver deposition, as the particle size of gold nanoparticles becomes larger, the color gradually changes from the red of nano-gold to the yellow of nano-silver and then to gray-black, which is very important for the development of a method for visual detection of alkaline phosphatase. It is of great practical significance, but due to the low visual resolution of gold nanoparticles, it is currently impossible to develop a high-resolution visual detection method for alkaline phosphatase

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alkaline Phosphatase Analysis Method Based on Gold Nanorod Long Silver Shell
  • Alkaline Phosphatase Analysis Method Based on Gold Nanorod Long Silver Shell
  • Alkaline Phosphatase Analysis Method Based on Gold Nanorod Long Silver Shell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Preparation of Gold Nanorods

[0027] Firstly, a seed solution was synthesized. At room temperature, ice-bathed and freshly prepared sodium borohydride solution (0.01 M, 0.6 mL) was rapidly added to HAuCl 4 (0.01 M, 0.25 mL) and hexadecyltrimethylammonium bromide (0.1 M, 9.75 mL), and then used after standing at room temperature for 2 hours. The growth solution was then prepared by mixing cetyltrimethylammonium bromide solution (0.1 M, 40 mL), HAuCl 4 solution (0.01 M, 2 mL), AgNO 3 solution (0.01 M, 0.400 mL), HCl solution (0.800 mL), and L-ascorbic acid solution (0.1 M, 0.32 mL) were mixed evenly. Finally, 200 mL of the seed solution was added to the above growth solution, and left at room temperature overnight. The next day, the synthesized gold nanorods were centrifuged at 7000 rpm for 20 min and dispersed in 10 mL of water. Such as figure 2 As shown in (a), the UV-vis spectrum of the synthesized gold nanorods has two absorption peaks (507 nm and 896 nm). ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
lengthaaaaaaaaaa
diameteraaaaaaaaaa
absorbanceaaaaaaaaaa
Login to View More

Abstract

The invention discloses an alkaline phosphatase analysis method based on gold nanorod long silver shells, and belongs to the technical field of nanometer materials and biological analysis. In this method, after incubating gold nanorods, buffer solution containing L-ascorbic acid-2-phosphate, silver nitrate solution and target alkaline phosphatase, the color change of the detection solution is recorded for visual semi-quantitative detection or with Quantitative detection by UV-Vis spectrophotometer. The method can carry out high-resolution colorimetric semi-quantitative detection on the alkaline phosphatase concentration by naked eyes, and can also carry out quantitative detection by an ultraviolet-visible spectrophotometer. The invention relates to a gold nanorod with simple preparation process, low cost, simple colorimetric detection method, high resolution and high sensitivity, and provides a simple, low-cost, high-resolution, sensitive and stable visual detection for clinical biomolecular detection new method.

Description

technical field [0001] The invention relates to an alkaline phosphatase analysis method based on gold nanorods and long silver shells, belonging to the technical field of nanometer materials and biological analysis. Background technique [0002] Alkaline phosphatase (ALP) is an enzyme present in human serum that can be used as an indicator of hepatobiliary and bone diseases, as well as breast and prostate cancer [Y. Liu et al., Anal. Chem., 2008, 80: 8605]. Moreover, due to the high catalytic activity, high stability, low price, and broad substrate specificity of alkaline phosphatase, it has become one of the most commonly used enzyme labels for immunoassays, aptamer-based assays, gene Analysis and related affinity analysis to detect toxins, proteins, nucleic acids, drugs, etc. [A. Hayat et al., Anal. Chem., 2013, 85: 10028]. Therefore, it is particularly important to detect alkaline phosphatase activity. So far, various methods and strategies based on different signal ge...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N21/78
Inventor 唐点平高壮强许铭棣林幼秀周倩
Owner FUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com