300 results about "Dextrose solution" patented technology

The invention discloses a method for preparing two-dimensional silver nanosheets of different shapes. Firstly, a silver mirror reaction is adopted, glucose serves as a reducing agent, a quantitative silver-ammonia solution and the quantitative glucose solution are sequentially added into a beaker to be mixed, then cetyl trimethyl ammonium bromide serves as a surface active agent, the cetyl trimethyl ammonium bromide solution is added into the mixed solution, then high-temperature reaction is carried out on the mixed solution, and a solution of the two-dimensional silver nanosheets of the different shapes is prepared by adjusting the concentration ratio of the cetyl trimethyl ammonium bromide solution and the silver-ammonia solution. The method has the advantages that operation is easy, cost is low, equipment is simple, and the prepared silver nanosheets are uniform in grain size, good in water solubility and free of obvious gathering.

The invention discloses a culture method for high-efficiency human follicle stimulating hormone expression CHO cells and belongs to the technical field of bio-pharmaceuticals. According to the culture method, seed cells are prepared into cell suspension, and subculture is performed after resuspension through a serum-free basal culture medium to obtain seed suspension; the obtained seed suspension is inoculated into a bioreactor to be cultured, the serum-free basal culture medium is fed at regular intervals in the culture process, growth situations of the cells are monitored, glucose solution is supplemented, and the cells are harvested after culture. According to the method, the serum-free basal culture medium is used throughout the process, culture in a fermentation tank is performed after reviving and amplification through bottle shaking, the process flow is simple, the cell culture density is high and can be as high as 4*107/mL , the cell survival rate reaches more than 98%, maintaining time under high density and a high survival rate is long, the yield of end products can reach 40mg/L, and the method is applicable to industrial production.

The invention discloses a surface plasma resonance probe with a silver-gold core-satellite structure and a preparation method thereof. The preparation method comprises the steps of: firstly, sequentially and quickly adding a glucose solution and a hexadecyl trimethyl ammonium bromide solution into a quantitative silver ammonia solution for reacting to prepare a nano silver cube solution; and then, standing a clean glass substrate in the nano silver cube solution to prepare a nano silver cube glass substrate; fixing single stranded DNA molecules on the prepared nano silver cube glass substrate; hybridizing the single stranded DNA with a miRNA molecule; and finally, preparing gold nanoparticles and decorating the gold nanoparticles at one end of the miRNA molecule to prepare the surface plasma resonance probe with a silver-gold core-satellite structure and capable of being applied in early detection of lung cancer. The particle diameter of the prepared surface plasma resonance probe is 50-70nm, the scattered spectrum peak is located at 450-650nm and the detection sensitivity is as high as 10nM.

The invention discloses a preparation method of carbon spheres, which comprises the following steps: s1. preparing a solution with the pH value of 3-10 from acid and alkali; s2. adding glucose into the solution obtained in the step s1 to prepare a 1-10g/100ml glucose solution; s3. putting the glucose solution obtained in the step s2 into a hydrothermal kettle, keeping the temperature at 160-200 DEG C for 2-20 hours, and cooling to room temperature; and s4. washing and drying the product obtained in the step s3 to obtain the target product. The method implements mass preparation and particle size control of carbon spheres with particle size of less than 100nm, does not have the participation of any organic solvent and toxic chemical reagent in the reaction process, can effectively avoid the problems of structure defects and environmental pollution caused by impurity introduction, and can be widely used for biochemical and biological diagnosis due to the nontoxic characteristic of the carbon spheres. The whole preparation process is simple to operate, has the advantages of high controllability, favorable repetitiveness and environment friendliness, and is suitable for large-scale production.

The invention discloses a preparation method and applications of a carbon dot/graphite like phase carbon nitride composite photocatalyst. The preparation method comprises following steps: 1, carbon dots are prepared, wherein a glucose solution and an alkaline solution are subjected to mixing ultrasonic treatment so as to obtain a mixed solution, the pH value of the mixed solution is adjusted withhydrochloric acid to 6 to 8, absolute ethyl alcohol is added wit stirring, magnesium sulfate is added, stirring is carried out, an obtained mixture is allowed to stand, and is filtered so as to obtaina settled solution, the settled solution is heated to 50 to 100 DEG C until the color of the settled solution is changed and a solid substance is generated, the solid substance is filtered, and is grinded into powder so as to obtain carbon dots; 2, the carbon dots are mixed with melamine at a mass ratio of (0.01-5) : (1-100), and an obtained product is delivered into a muffle furnace for calcining at 200 to 700 DEG C so as to obtain the CDs/g-C3N4 composite photocatalyst. Carbon dots possess excellent up conversion properties, so that the CDs/g-C3N4 composite photocatalyst is capable of conversing absorbed light with long wave length into light with short wave length, increasing visible light utilization rate, and increasing photocatalytic activity.

The invention relates to an electrochemical detection method for measuring the concentration of trivalent arsenic in a water system without electricity enrichment, which comprises utilizing a three-electrode system which is formed by a gold electrode, a reference electrode and a counter electrode to oxygenize the surface of the gold electrode to be gold trioxide in a detection pool, immersing gold trioxide into beta-D-glucose solution, deacidizing gold trioxide to be a nano-gold film, immersing gold electrode into 0.1M phosphate buffer solution (the concentration detecting range is 10.0nM-5.0muM) which is contained with trivalent arsenic under test, adsorbing trivalent arsenic through the nano-gold film, leading arsenic to be enriched on the surface of the nano-gold film, inserting the gold electrode and the reference electrode which are enriched with arsenic into the electrochemical detection tank, starting an electrochemical working station to scan, leading arsenic which is enrichedin the process of scanning to do redox reaction on the surface of the gold electrode, getting the oxidation peak current value which has corresponding relationship with trivalent arsenic, thereby measuring the concentration of arsenic solution, achieving the detection of trivalent arsenic in high sensitivity, and solving the problem of applying high-concentration acid medium.

Disclosed are a liquid product of botulinum toxin type A and a method for conserving the potency of botulinum toxin type A using a dextrose solution. Free of a stabilizer, such as albumin or gelatin, the liquid product of botulinum toxin type A completely excludes the possibility of cross infections such as AIDS and bovine spongiform encephalopathy. In addition, botulinum toxin type A is preserved as a liquid product in combination with a dextrose solution and can be clinically used as is, without the aid of physiological saline. Therefore, the liquid product enjoys the advantage of being convenient for use and avoiding a decrease in the potency as occurs upon dilution with physiological saline. Serving as a natural preserving and stabilizing agent, the dextrose solution allows botulinum toxin type A to be stored and distributed in the form of liquid phase over a long period of time and conserves the potency of the toxin at a constant level, which in turn guarantees constant clinical results.

The invention provides a preparation method for a high-flux solvent-resistant nanometer mixed nanofiltration membrane based on natural materials, belonging the field of preparation methods for membranes. The preparation method comprises the following steps: 1, preparing a water-free polyimide ultrafiltration base membrane; 2, preparing an alkanolamine solution of hexylenediamine and putting the membrane prepared in the previous step into the prepared alkanolamine solution of hexylenediamine for cross-linking modification; 3, preparing a buffer solution, adding dopamine, and soaking cross-linked polyimide in a buffer solution of dopamine; 4, synthesizing a metal-organic framework (Zr-MOF); 5, preparing an acyl chloride solution; and 6, preparing a glucose solution, soaking the dopamine-modified polyimide membrane in the solution of glucose and Zr-MOF so as to obtain the nanometer mixed nanofiltration membrane based on natural materials. The nanometer mixed nanofiltration membrane prepared in the invention has the advantages of great organic-solvent permeation flux, high dye retention rate, etc. The preparation method is applied to the field of preparation of nanofiltration membranes.

An apparatus for ultrafiltration of a patient being overhydrated due to congestive heart failure, comprising a tube set including a connector for connection to a patient line for access to the peritoneal cavity of the patient. A flow pump is arranged for addition and removal outflow and inflow (recirculation) of fluid from/to the peritoneal cavity. An osmotic agent peristaltic pump is arranged for replenishment of glucose solution to the fluid added to the peritoneal cavity for promoting ultrafiltration. The glucose is replenished intermittently for keeping a concentration of glucose substantially constant in the peritoneal cavity. The flow pump comprises a pressure chamber with rigid walls and a flexible pump bag arranged therein. An air pump pressurizes the chamber for outflow of fluid from the peritoneal cavity by a sub pressure and inflow of fluid to the peritoneal cavity by an overpressure, which pressures are maintained within safe limits.

The invention relates to a preparation method of a solid amino carbon dioxide capture material. The method comprises the following steps: carrying out hydrothermal reaction on a glucose solution to prepare carbon spheres, dissolving the carbon spheres, cetyl trimethylammonium bromide and tetraethyl orthosilicate in a methanol-ethanol mixed solution, adding stronger ammonia water to react, filtering, roasting to prepare silicon oxide hollow spheres, adding the silicon oxide hollow spheres into an organic amine-ethanol mixed solution or a surfactant-organic amine-ethanol mixed solution, and drying to obtain the silicon-oxide-hollow-sphere-carried amine-type adsorbent. The method has the advantages of accessible raw materials, low price, mild preparation conditions, simple steps and high efficiency, and can implement large-scale production.

The invention belongs to the field of a biomedical material, which is mainly applied to the preparation of a compound dosage form both of bone morphogenetic protein 2 active peptide and hydroxyapatite, wherein a sequence of the bone morphogenetic protein 2 active peptide is represented by SEQ ID NO: 1-10. A preparation method provided by the invention comprises the following steps: dissolving the bone morphogenetic protein 2 active peptide into normal saline or 5% of glucose solution, and then adding a hydroxyapatite support, combining the bone morphogenetic protein 2 active peptide on the surfaces of the hydroxyapatite particles to obtain the necessary compound dosage form both of the bone morphogenetic protein and hydroxyapatite after centrifugal separation, washing and drying, so as to obtain a human hard tissue repair material provided by the invention.

The invention discloses a preparation method of amino acid and glucose injection packaged by a dual-chambered bag. The method comprises the steps of using glucose and injection water to prepare a solution; using amino acid and injection water to prepare a solution and charging nitrogen into the solution; feeding the glucose solution and the amino acid solution to two chambers of the dual-chambered bag simultaneously; vacuumizing and then filling nitrogen; loading the filled dual-chambered bag into an outer bag with high barrier; vacuumizing, filling nitrogen for several minutes, and packaging; and putting the packaged dual-chambered bag to a sterilizing device to sterilize at 115-125 DEG C. The amino acid and glucose injection provided by the invention is packaged by the dual-chambered bag. Because the amino acid solution and the glucose solution are fed in two chambers separated by cold solder joint, Maillard reaction or other side effects between amino acid and glucose due to long-term contact during the sterilization and storage are prevented, and then the usage safety and efficiency of the product can be ensured.

The invention relates to a preparation method of a highly-effective Cr (VI) absorption carbon-aluminum oxide composite material. The preparation method of the highly-effective Cr (VI) absorption carbon-aluminum oxide composite material comprises hydrolyzing aluminum alkoxide under weakly acidic conditions to obtain pseudo-boehmite sol, uniformly mixing the pseudo-boehmite sol with a glucose solution with a certain amount of lauryl sodium sulfate dissolved in, adding in a certain amount of ethyl alcohol, and performing water boiling, separation, drying and baking to obtain the highly-effective Cr (VI) absorption carbon-aluminum oxide composite material. The highly-effective Cr (VI) absorption carbon-aluminum oxide composite material achieves a removal rate up to 98.2% for Cr (VI) solutions with the concentration lower than 80 mg/L. The prepared highly-effective Cr (VI) absorption carbon-aluminum oxide composite material greatly improves the absorbing capacity and the absorbing rate of toxic Cr (VI); when applied in a 100 mg/L mixed solution of Cr (VI), Cd (II), Cu (II), Zn (II) and Ni (II), the highly-effective Cr (VI) absorption carbon-aluminum oxide composite material also achieves excellent selective absorbability of Cr (VI) and reaches an absorbing capacity up to 76.5 mg/g, and for a single Cr (VI) solution with the same initial concentration, the highly-effective Cr (VI) absorption carbon-aluminum oxide composite material achieves an absorbing capacity of 78.58 mg/g.

The invention discloses a method for preparing polyhydroxybutyrate (PHB), which is implemented by taking cupriavidus strains sh-1 (preservation No.: CGMCC No. 4815) as strains for preparing PHB. The method comprises the following steps: inoculating the strains in a slant culture medium, then culturing the strains for 24 hours at a temperature of 30 DEG C; taking slant cultures, then inoculating the slant cultures in a conical flask filled with a seed culture medium, and under the condition of shaking the conical flask at a rotating speed of 180 r/min, culturing the slant cultures for 24-36 hours at a temperature of 30 DEG C; inoculating the obtained seed liquid in a fermentation medium according to an inoculation quantity of 10%, and stirring the obtained product at a rotating speed of 300-500 r/min, then after fermenting the seed liquid 72 hours at a ventilatory capacity of 50-200 L/min and a fermentation temperature of 28-35 DEG C, stopping the operation of fermentation; and in the process of fermentation, when the dissolved oxygen content of the fermentation medium is increased to 80% of saturated dissolved oxygen content, adding a glucose solution (concentration: 10-300 g/L) into the fermentation medium, then when the dissolved oxygen content of the obtained solution drops to 50%, stopping the operation of adding. By using the method disclosed by the invention, the high-density fermentation and high yield of polyhydroxybutyrate can be realized.

The invention relates to a modified electrode for detecting dextrose concentration in absence of enzyme, as well as a preparation method thereof. Firstly, a carbon nano-tube is modified on the surface of a glassy carbon electrode; and then a cyclic voltammetry is used for carrying out electrochemical deposition of nickel hexacyanoferrate on the electrode surface modified by the carbon nano-tube, thereby obtaining the modified electrode modified compositely by the carbon nano-tube and nickel hexacyanoferrate. The modified electrode has good response to dextrose solution in absence of enzyme, the detection lower limit of the dextrose concentration is 1.6*10mol/L; when the dextrose concentration ranges from 3.32*10M/L to 4.95*10M mol/L, the obtained response current has a good linear relation with the dextrose concentration, and the prepared electrode has excellent anti-interference ability.

The invention discloses a preparation method of a graphene oxide coated carbon microsphere composite material. The method comprises the following steps: preparing a dextrose solution as a carbon source; performing hydrothermal reaction under an acidic condition; washing the obtained product in a plurality of times; performing suction filtering; drying to obtain carbon microspheres; dissolving thecarbon microspheres in a mixed solution consisting of water and absolute ethyl alcohol; then adding a mixed solution of a silane coupling agent and the absolute ethyl alcohol; performing condensing and reflowing under a room temperature or a heating condition; washing the obtained product in a plurality of times; performing suction filtering; drying to obtain modified carbon microspheres; adding graphene oxide to DMF; then adding an activating agent; ultrasonically treating to obtain a graphene oxide solution; adding the modified carbon microspheres to the graphene oxide solution; ultrasonically processing to obtain an uniform mixed solution A; adding a catalyst to the mixed solution A; performing condensing and reflowing under a heating condition; washing the obtained product in a plurality of times; and performing suction filtering and drying to obtain the graphene oxide coated carbon microsphere composite material.

The invention relates to a method for synthesizing anode material LiMn0.7Fe0.3PO4 for a lithium ion cell through a microwave method. After Li2CO3, Fe2O3 and phosphate are metered according to a certain mole ratio, the phosphate is made into phosphate solutions, citric acid is added to the phosphate solutions, and water solutions of the citric acid and the phosphate are prepared. Li2CO3 and Fe2O3 are added, and even stirring is carried out to obtain paste mixtures. A first precursor is obtained after ageing and microwave heat processing. Li2CO3, MnCO3 or MnO2 and the phosphate are used as raw materials to obtain a second precursor in the same way, the first precursor and the second precursor are mixed, glucose solutions are added, and a paste precursor is obtained. Finally, the anode material is obtained through microwave sintering. The charging and discharging capacity and circulating frequency of the anode material for the cell are good. The cell is high in capacity, and good performance is achieved. Accurate control can be achieved through microwave processing, the process is simple, pollution is avoided, and environmental protection is facilitated.

The present invention relates to the field of medical technology. More specifically, the present invention relates to a preparation of taxanes for intravenous administration, which consists of two parts: a drug solution and an emulsion. Said drug solution consists of paclitaxel or docetaxel, a pH regulator and a solvent for injection, wherein said solvent for injection is an organic solvent. Said emulsion includes a fat emulsion and is composed of oil for injection, an emulsifier, an antioxidant, an isotonic regulator, a stabilizer, a pH regulator and water for injection. When used, the drug solution at the clinical dosage can be added and mixed homogeneously in the emulsion to perform intravenous drip directly; or the drug solution at the clinical dosage can also be firstly added into the emulsion with no less than 5 times volume of the drug solution and then a predetermined amount of normal saline or glucose solution for injection is added to perform intravenous drip. The preparation of the present invention does not contain solubilizer and has advantages of little toxicity, safety, effectiveness, stability and economy. The fat emulsion is also used as a nutritional replenishment, thus achieving a better therapeutic effect. In addition, the normal saline or glucose solution for injection can be used to replace a considerable amount of the emulsion, which makes the preparation, therefore, not only cost-efficient, but also convenient for transportation and storage in practice.

The invention discloses a liquid mixing and uniform shaking device for preparation of a glucose solution for injection. The liquid mixing and uniform shaking device comprises a base, a first drive motor, a material mixing box, a filtering box and a second drive motor; a joint bearing is rotationally mounted on a rotating base, and a spherical surface on the upper side of the joint bearing is fixedly mounted at the bottom of a fixed seat; material distribution wheels are rotationally mounted under a first feeding opening and a second feeding opening through rotating shafts; the filtering box ismounted on a slide bar in a sliding manner; a cylinder output shaft is horizontally connected with the filtering box leftwards. The liquid mixing and uniform shaking device disclosed by the inventionhas the beneficial effects that as the material distribution wheels are arranged in the liquid mixing and uniform shaking device, and material storage cavities in the material distribution wheels areconstant in volume, interval quantitative feeding can be realized, so that the excessive solution addition from any side is avoided. Therefore, the concentration of the prepared glucose solution is ensured to meet the requirements; meanwhile, after being mixed, the glucose solution can be uniformly shook again, so that the mixing degree of the glucose solution is improved, and the situation of layering due to long-time placement is avoided.

The invention relates to a long-acting controlled-release antibacterial mildew-proof material and a preparation method thereof, aiming at overcoming the defects that inorganic antibacterial agent nanometer ZnO is poor in effect of inhibiting mould and an organic mildew preventive agent is short in antibacterial time and easy to migrate. The invention provides an inorganic/organic composite antibacterial mildew-proof material, which is prepared by the following steps: arranging a glucose solution in a hydrothermal reaction kettle for reacting, centrifugally separating and washing, and drying to obtain carbon microspheres; arranging the carbon microspheres in an aqueous solution of zinc acetate, performing ultrasonic treatment, aging, centrifugally separating, washing, drying and calcining, thereby obtaining hollow mesoporous ZnO microspheres; and soaking the hollow mesoporous ZnO microspheres with an organic mildew preventive agent solution, and drying, thereby obtaining drug-loading mesoporous ZnO microspheres, namely the long-acting controlled-release antibacterial mildew-proof material. The long-acting controlled-release antibacterial mildew-proof material prepared by the invention has the advantages of non-toxicity, environment friendliness, good antibacterial and mildew-proof effects, long antibacterial time, good thermal stability and the like and can be widely applied to antibacterial and mildew-proof treatment in the fields of leather, textiles, papermaking and furniture.