Method for photoelectrochemically detecting organophosphorus pesticide based on bismuth oxybromide/bismuth sulfide semiconductor heterojunction generated by biological induction

An organophosphorus pesticide and photoelectrochemical technology, which is applied in the direction of measuring devices, scientific instruments, and material analysis by electromagnetic means, can solve the problems of low-cost OPs detection, expensive, cumbersome, etc., and achieve photoelectric signal enhancement and promote separation. , Improve the effect of photocurrent intensity

Active Publication Date: 2020-12-18
JIANGXI NORMAL UNIVERSITY
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Problems solved by technology

However, the disadvantages of these methods are the need for expensive instruments, specialized ope

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  • Method for photoelectrochemically detecting organophosphorus pesticide based on bismuth oxybromide/bismuth sulfide semiconductor heterojunction generated by biological induction
  • Method for photoelectrochemically detecting organophosphorus pesticide based on bismuth oxybromide/bismuth sulfide semiconductor heterojunction generated by biological induction
  • Method for photoelectrochemically detecting organophosphorus pesticide based on bismuth oxybromide/bismuth sulfide semiconductor heterojunction generated by biological induction

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Embodiment 1

[0039] (1) 0.3g of Bi(NO 3 ) 3 ·5H 2 O was dissolved in 10 mL of ethylene glycol monomethyl ether, and then slowly added 0.6 g of 1-dodecyl-3-methylimidazolium bromide ([C 12 Mim]Br), stirred evenly and ultrasonically treated for 20min; the mixture was transferred to a stainless steel autoclave lined with Teflon, and heated to 160°C for 24h; after cooling, a milky white product could be observed at the bottom of the autoclave, alternately After washing with deionized water and ethanol three times, the BiOBr powder was obtained after drying overnight at 80 °C.

[0040] (2) Ultrasonic disperse 4 mg of BiOBr powder prepared in step (1) in 1 mL of ultrapure water, and drop 10 μL on the surface of the ITO electrode. After drying at room temperature, a BiOBr / ITO electrode was obtained.

[0041] (3) Add 10 μL of biotin-modified helper DNA (S 1, 1.0μM) and 10μL avidin-modified magnetic beads (Strep-MB, 5mg mL -1 ), after incubating at 37°C for 20min, add 10μL of malathion aptame...

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Abstract

The invention discloses a method for photoelectrochemically detecting an organophosphorus pesticide based on a BiOBr/Bi2S3 semiconductor heterojunction generated by biological induction. The method comprises the following steps: mixing malathion with avidin-modified magnetic beads, biotin-modified auxiliary DNA and a malathion aptamer, incubating, removing part of the malathion aptamer by using malathion to recover the single strand of the auxiliary DNA, triggering by using the auxiliary DNA to form G-rich dsDNA, and embedding the dsDNA into Hemin and MnTMPyP to catalyze a Na2S2O3 and H2O2 mixed solution to generate H2S. H2S reacts with BiOBr modified on an electrode to generate a BiOBr/Bi2S3 compound, so that the photocurrent is greatly increased, the change of the photocurrent and the concentration of a target object are in a linear relationship in a certain range, HCR signal amplification is designed on the basis of specific recognition of malathion and an aptamer thereof, biocatalysis is utilized to induce a BiOBr/Bi2S3 semiconductor heterojunction to amplify a signal, therefore, high-sensitivity and high-selectivity photoelectric method detection of malathion is realized.

Description

technical field [0001] The invention relates to a detection method of organophosphorus pesticides, in particular to a BiOBr / Bi 2 S 3 The invention relates to a semiconductor heterojunction photoelectrochemical detection method for organophosphorus pesticides, which belongs to the field of photoelectrochemical bioanalysis. Background technique [0002] Pesticides play a key role in modern agriculture as weapons against various pests including weeds, insects, rodents and fungi. Among them, organophosphorus pesticides (OPs) are important pesticides that are widely used as insecticides and nerve agents due to their high efficiency and broad spectrum. However, the widespread use of OPs can severely pollute water resources, soil, and agricultural products. And the residues can be transferred to the human body through the food chain. It is worth noting that OPs will cause harm to the human central nervous system (CNS) by inhibiting the activity of acetylcholinesterase (AChE). ...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N21/63
CPCG01N27/3276G01N21/63Y02P20/133
Inventor 汤娟刘丽萍熊鹏媛李晶晶曾志瑶
Owner JIANGXI NORMAL UNIVERSITY
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