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A preparation method of competitive electrochemical immunosensor based on mofs material

An immune sensor and electrode technology, applied in the direction of material electrochemical variables, scientific instruments, analytical materials, etc., can solve the problems of unfavorable electron transfer and poor electron transfer ability of MILs, and achieve short response time, improved sensitivity, and wide linear range Effect

Active Publication Date: 2020-11-24
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Different from the UiO series, although the MILs series also has a larger pore size and specific surface area, the electron transfer ability of MILs is poor, which is not conducive to electron transfer.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 The preparation steps of a UiO-66 / Au composite material based on MOFs material competitive immunosensor are as follows:

[0036] (1) Preparation of UiO-66

[0037] 1.25 g of zirconium tetrachloride, 1.23 g of terephthalic acid and 10 mL of hydrochloric acid were ultrasonically dissolved in 100 mL of DMF, then transferred to an autoclave, and reacted at 120 °C for 24 h. After the reaction, the resulting mixture was Centrifuge at 9000 r / min for 10 min, wash with DMF and methanol three times, discard the supernatant, and place the centrifuge tube in a 60 o C is dried in a vacuum oven, and finally is ground to obtain a white solid powder UiO-66;

[0038] (2) Preparation of UiO-66 / Au

[0039]Weigh 0.1 g UiO-66 and ultrasonically disperse in 10 mL methanol at room temperature to obtain a white uniform emulsion, add 0.3 mL chloroauric acid to mix, stir at room temperature for 5 h to obtain a yellow emulsion, weigh 0.07 g boron After sodium hydride was ultrasonical...

Embodiment 2

[0040] Example 2 The preparation steps of a UiO-66 / Au composite material based on MOFs material competitive immunosensor are as follows:

[0041] (1) Preparation of UiO-66

[0042] 1.5 g of zirconium tetrachloride, 2.0 g of terephthalic acid and 15 mL of hydrochloric acid were ultrasonically dissolved in 100 mL of DMF, then transferred to an autoclave, and reacted at 120 °C for 24 h. After the reaction, the obtained mixture was Centrifuge at 9000 r / min for 10 min, wash with DMF and methanol three times, discard the supernatant, and place the centrifuge tube in 60 o C is dried in a vacuum oven, and finally is ground to obtain a white solid powder UiO-66;

[0043] (2) Preparation of UiO-66 / Au

[0044] Weigh 0.15 g of UiO-66 and ultrasonically disperse it in 10 mL of methanol at room temperature to obtain a white uniform emulsion, add 0.35 mL of chloroauric acid to mix, stir at room temperature for 5 h to obtain a yellow emulsion, weigh 0.06 g of boron After sodium hydride was...

Embodiment 3

[0045] Example 3 The preparation steps of a UiO-66 / Au composite material based on MOFs material competitive immunosensor are as follows:

[0046] (1) Preparation of UiO-66

[0047] 1.5 g of zirconium tetrachloride, 1.0 g of terephthalic acid and 20 mL of hydrochloric acid were ultrasonically dissolved in 100 mL of DMF, then transferred to an autoclave, and reacted at 120 °C for 12 h. After the reaction, the obtained mixture was Centrifuge at 9000 r / min for 10 min, wash with DMF and methanol three times, discard the supernatant, and place the centrifuge tube in 60 o C is dried in a vacuum oven, and finally is ground to obtain a white solid powder UiO-66;

[0048] (2) Preparation of UiO-66 / Au

[0049] Weigh 0.2 g UiO-66 and ultrasonically disperse in 10 mL methanol at room temperature to obtain a white uniform emulsion, add 0.4 mL chloroauric acid to mix, stir at room temperature for 5 h to obtain a yellow emulsion, weigh 0.065 g boron After sodium hydride was ultrasonically ...

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Abstract

The invention relates to preparation and application of a competitive immunosensor, in particular to a competitive immunosensor which takes a composite material of UiO-66 and gold nanoparticles (UiO-66 / Au) as a sensing platform and NH2-MIL-125 as a marker, and belongs to the technical field of novel functional materials and biosensing. The UiO-66 / Au has excellent electrical conductivity and largespecific surface area, so that the electron transfer process can be effectively promoted and the roles of amplifying the current signal and increasing the sensitivity of the sensor are played; and theNH2-MIL-125 synthesized by utilizing an one-pot method has large steric hindrance to ensure that the electron transfer can be hindered and the current signal is reduced, so that the changing value ofthe current signal is increased, the content of a target can be detected by utilizing the change of the electrochemical signal by the sensor; and therefore, the stability and the sensitivity of the immunosensor are significantly increased, the detection range is expanded and the detection limit is reduced.

Description

technical field [0001] The present invention relates to the preparation and application of an electrochemical immunosensor, specifically a UiO-66 / Au sensor platform, NH 2 -MIL-125 is a competitive immunosensor of conjugated markers, and the invention belongs to the technical field of new functional materials and biosensing. Background technique [0002] Carcinoembryonic antigen (CEA) is an antigen belonging to the surface structure of tumor cells [6] , which is formed in the cytoplasm, can penetrate out of the cell through the cell membrane and enter the surrounding body fluid, so it can be detected in a variety of body fluids. As the most common tumor marker, CEA is widely used in the diagnosis and monitoring of various digestive system tumors. In organisms, all endoderm-derived malignant tumors such as colon, rectum, esophagus, stomach, liver and pancreas CEA exists in the serum of patients with cancer such as cancer, and its content is significantly higher than that of ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/30G01N33/531
CPCG01N27/30G01N27/3278G01N33/531
Inventor 曹伟李璇魏琴李丽李悦源苗俊聪
Owner UNIV OF JINAN
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