Method for detecting trivalent chromic ions by using rhodamine fluorescent probe

A technology of fluorescent probe and trivalent chromium, which is applied in the field of fluorescent probe material detection, can solve problems affecting the accuracy and sensitivity of inspection, difficulty in grasping the volume of boiled water samples, unfavorable real-time monitoring, etc. The effect of low line and good use effect

Inactive Publication Date: 2016-08-31
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The traditional detection of trivalent chromium ions requires the help of large instruments, most of which use voltammetry, atomic absorption spectrometry, surface plasmon enhanced resonance scattering, and colorimetric methods that have attracted more attention recently, although these methods can be used to detect trivalent chromium ions. Chromium, but limited by bulky instrumentation
For example, ferrous ammonium sulfate titration can be used to detect trivalent chromium ions, but the use of this method is limited due to the large amount of vanadium in the sample that has a great impact on the system
The common diphenylcarbahydrazide spectrophotometric method is widely used because of its simple equipment, precision, and low detection line. However, this method needs to be boiled and digested by heating, which is easy to cause bumping, and the volume of the boiled water sample is difficult to grasp, thus Affect the accuracy and sensitivity of inspection
The inductive coupling method plasma torch is a kind of spectral analysis method of excitation light source, which has been widely used in routine analysis, and has the advantages of fast analysis speed and high precision. real-time monitoring

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  • Method for detecting trivalent chromic ions by using rhodamine fluorescent probe
  • Method for detecting trivalent chromic ions by using rhodamine fluorescent probe
  • Method for detecting trivalent chromic ions by using rhodamine fluorescent probe

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Embodiment 1

[0042] (1) Dissolve rhodamine-based fluorescent probes in acetonitrile solvent to prepare a 10 μM fluorescent probe solution, dissolve chromium nitrate in acetonitrile solvent to prepare a 10 μM trivalent chromium ion solution, and prepare 1 mL of 10 μM fluorescent probe solution Add it to 1mL 10μM trivalent chromium ion solution, test the ultraviolet change spectrum of the fluorescent probe solution before and after adding the trivalent chromium ion solution, the results are as follows figure 1 shown.

[0043] (2) Dissolve rhodamine-based fluorescent probes in acetonitrile solvent to prepare a 10 μM fluorescent probe solution, dissolve chromium nitrate in acetonitrile solvent to prepare a 10 μM trivalent chromium ion solution, and prepare 1 mL of 10 μM fluorescent probe solution Add to 1mL 10μM trivalent chromium ion solution, test the fluorescence excitation and emission change patterns of the fluorescent probe solution before and after adding the trivalent chromium ion solu...

Embodiment 2

[0053] A method for detecting trivalent chromium ions by using a rhodamine fluorescent probe, the specific steps are:

[0054] Step 1: Prepare rhodamine-based fluorescent probe solution: weigh a certain molar amount of rhodamine-based fluorescent probe in acetonitrile solvent to prepare 100mL with a concentration of 1x10 -3 M fluorescent probe solution, then take 1mL of the above concentration to be 1x10 -3 The fluorescent probe solution of M was added to a 10mL dry volumetric flask, then fixed to volume with acetonitrile solvent and shaken to obtain 1x10 -4 M fluorescent probe solution, take 1mL of the above concentration is 1x10 -4 The fluorescent probe solution of M was added to a 10mL dry volumetric flask, then fixed to volume with acetonitrile solvent and shaken to obtain 1x10 -5 The rhodamine fluorescent probe solution of M;

[0055] Step 2: Preparation of trivalent chromium ion solution: weigh a certain molar amount of trivalent chromium compound chromium nitrate in ac...

Embodiment 3

[0060] A method for detecting trivalent chromium ions by using a rhodamine fluorescent probe, the specific steps are:

[0061] Step 1: Prepare rhodamine-based fluorescent probe solution: the rhodamine-based fluorescent probe refers to the synthesis method of 201510869087.0 "Reactive rhodamine-based fluorescent probe for detecting mercury ions and its preparation and application". The preparation method of the rhodamine-based fluorescent probe solution is as follows: a certain molar amount of rhodamine-based fluorescent probe is configured in an acetonitrile solvent so that the concentration of 100mL is 1×10 -3 M fluorescent probe solution, then take 1mL of the above concentration to be 1x10 -3 The fluorescent probe solution of M was added to a 10mL dry volumetric flask, then fixed to volume with acetonitrile solvent and shaken to obtain 1x10 -4 M fluorescent probe solution, take 1mL of the above concentration is 1x10 -4 The fluorescent probe solution of M was added to a 10mL...

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Abstract

The invention provides a method for detecting trivalent chromic ions by using a rhodamine fluorescent probe, and is characterized in that the method includes the steps: preparing trivalent chromic ion solutions with different concentrations, allowing to stand, under a condition of the excitation wavelength of 560 nm, detecting the fluorescence intensity at the emission wavelength of 582 nm, and with the concentration of chromium ions as an abscissa and the fluorescence intensity as an ordinate, mapping to obtain a linear relationship curve; adding a rhodamine fluorescent probe solution to an NaOH / NaH2PO4 buffer solution to obtain a buffer system containing the fluorescent probe, adding a to-be-tested trivalent chromic ion solution, allowing to stand, under a condition of the excitation wavelength of 560 nm, detecting the fluorescence intensity at the emission wavelength of 582 nm, and according to the linear relationship curve obtained in the step 4, calculating the concentration of the chromium ions. The fluorescent probe can selectively identify the trivalent chromic ions, is free from the interference of other common ions, and is high in fluorescence intensity.

Description

technical field [0001] The invention belongs to the field of fluorescent probe material detection methods, in particular to a method for detecting trivalent chromium ions by using a rhodamine fluorescent probe. Background technique [0002] Chromium is a common element in nature and widely distributed on the earth. The valences of common chromium compounds are mainly +3 and +6, which play an extremely important role in ensuring human health, regulating plant growth, and promoting industrial development. In terms of human health, trivalent chromium is the main element to balance human and animal diets, and the daily requirement of human and animal bodies is about 50-200μg. Also, trivalent chromium plays a vital role in the metabolism of carbohydrates, proteins, fats and nucleic acids. First, chromium can inhibit mutations and has a certain anti-cancer effect; second, chromium can regulate sugar metabolism, which can prevent diabetes to a certain extent; third, chromium can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64G01N21/78
CPCG01N21/643G01N21/78
Inventor 光善仪徐曼曼赵刚徐洪耀
Owner DONGHUA UNIV
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