Method for detecting stannous ions by using rhodamine fluorescence probe

A fluorescent probe, tin ion technology, applied in the field of fluorescent probe material detection, achieves the effects of high fluorescence intensity, good selectivity and simple operation

Inactive Publication Date: 2016-08-31
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Regrettably, there are few reports of rhodamine-based fluorescent probes that recognize stannous ions

Method used

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  • Method for detecting stannous ions by using rhodamine fluorescence probe
  • Method for detecting stannous ions by using rhodamine fluorescence probe
  • Method for detecting stannous ions by using rhodamine fluorescence probe

Examples

Experimental program
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Effect test

Embodiment 1

[0042] (1) Dissolve rhodamine-based fluorescent probes in acetonitrile solvent to prepare a 10 μM fluorescent probe solution, dissolve rhodamine-based fluorescent probes in acetonitrile solvent to prepare a 10 μM stannous ion solution, and dissolve 1.0 mL of 10 μM Fluorescent probe solution was added in 1.0mL 10μM stannous ion solution, and the UV change spectrum of fluorescent probe solution was tested before and after adding stannous ion solution, and the results were as follows: figure 1 shown.

[0043] (2) Dissolve the rhodamine-based fluorescent probe in an acetonitrile solvent to prepare a 10 μM fluorescent probe solution, dissolve the rhodamine-based fluorescent probe in an acetonitrile solvent to prepare a 10 μM stannous ion solution, and dissolve 1.0 mL of 10 μM Fluorescent probe solution was added to 1.0mL 10 μ M stannous ion solution, and the fluorescence change spectrum of fluorescent probe solution was tested before and after adding stannous ion solution, and the ...

Embodiment 2

[0050] A kind of method utilizing rhodamine fluorescent probe to detect stannous ion, concrete steps are:

[0051] Step 1: Prepare rhodamine-based fluorescent probe solution: the rhodamine-based fluorescent probe refers to the synthesis method of 201510869087.0 "Reactive rhodamine-based fluorescent probe for detecting mercury ions and its preparation and application". The preparation method of the rhodamine-based fluorescent probe solution is as follows: a certain molar amount of rhodamine-based fluorescent probe is configured in an acetonitrile solvent so that the concentration of 100mL is 1×10 -3 M fluorescent probe solution, then take 1mL of the above concentration to be 1x10 -3 The fluorescent probe solution of M was added to a 10mL dry volumetric flask, then fixed to volume with acetonitrile solvent and shaken to obtain 1x10 -4 M fluorescent probe solution, take 1mL of the above concentration is 1x10 -4 The fluorescent probe solution of M was added to a 10mL dry volumet...

Embodiment 3

[0057] Step 1: Prepare rhodamine-based fluorescent probe solution: the rhodamine-based fluorescent probe refers to the synthesis method of 201510869087.0 "Reactive rhodamine-based fluorescent probe for detecting mercury ions and its preparation and application". The preparation method of the rhodamine-based fluorescent probe solution is as follows: a certain molar amount of rhodamine-based fluorescent probe is configured in an acetonitrile solvent so that the concentration of 100mL is 1×10 -3 M fluorescent probe solution, then take 1mL of the above concentration to be 1x10 -3The fluorescent probe solution of M was added to a 10mL dry volumetric flask, then fixed to volume with acetonitrile solvent and shaken to obtain 1x10 -4 M fluorescent probe solution, take 1mL of the above concentration is 1x10 -4 The fluorescent probe solution of M was added to a 10mL dry volumetric flask, then fixed to volume with acetonitrile solvent and shaken to obtain 1x10 -5 The rhodamine fluoresc...

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Abstract

The invention provides a method for detecting stannous ions by using a rhodamine fluorescence probe. The method comprises the following steps: adding a rhodamine fluorescence probe solution to a NaOH / NaH2PO4 buffer solution to obtain multiple fluorescence probe-containing buffer systems, respectively adding different volumes of a stannous ion solution to obtain stannous ion solutions with different concentrations, standing the stannous ion solutions, detecting the fluorescence intensity at an emission wavelength of 587nm under an excitation wavelength of 560nm, and mapping through adopting the concentration of the stannous ions as abscissa and the fluorescence intensity as ordinate to obtain a linear relation curve; and adding the rhodamine fluorescence probe solution to the NaOH / NaH2PO4 buffer solution to obtain a fluorescence probe-containing buffer system, adding a stannous ion solution to be detected, standing the obtained system, detecting the fluorescence intensity at an emission wavelength of 587nm under an excitation wavelength of 560nm, and calculating the concentration of the stannous ions. The method has the advantages of simple operation, fast detection, high sensitivity and good selectivity.

Description

technical field [0001] The invention belongs to the field of fluorescent probe material detection methods, in particular to a method for detecting stannous ions by using a rhodamine fluorescent probe. Background technique [0002] Stannous ions have a long history of application in the prevention of dental caries. As a trace element necessary for human physiological activities, a large amount of evidence shows that Sn 2+ In addition to effectively inhibiting the formation of cancer cells, it can also promote the synthesis of proteins and nucleic acids, promote the growth and development of the body, and participate in the biological reactions of various enzymes and flavin enzymes, thereby enhancing the stability of the internal environment. The lack of stannous may lead to poor growth and hearing loss, but the excessive accumulation of stannous will interfere with the metabolism of zinc ions, which will affect the respiratory and digestive systems. [0003] Stannous ions ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07D491/107G01N21/64
CPCC07D491/107C09K11/06C09K2211/1029C09K2211/1059C09K2211/1088G01N21/643
Inventor 光善仪徐曼曼赵刚徐洪耀
Owner DONGHUA UNIV
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