Immunochromatographic kit for joint detection of four enteroviruses and preparation method for immunochromatographic kit

A technology of combined detection and immunochromatography, applied in measurement devices, analytical materials, instruments, etc., can solve the problems of lack of long-term immune protection, no protection of norovirus antibodies, repeated infection, etc., and achieve easy control and detection line. Low, easy-to-use effect

Active Publication Date: 2016-08-17
BEIJING MOKOBIO LIFE SCI CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Norovirus antibodies have no significant protective effect, especially no long-term immune protection, which can easily cause repeated infections

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] An immunochromatographic kit for combined detection of four enteroviruses, comprising a housing with two viewing windows, a first test strip and a second test strip are placed in the housing, and the first test paper Both the strip and the second test strip include a plastic plate, a glass cellulose membrane arranged on the plastic plate, a nitrocellulose membrane arranged on the glass cellulose membrane, and a sample pad is arranged at one end of the nitrocellulose membrane, corresponding to The other end of the test strip is provided with a water-absorbing pad, and the nitrocellulose membrane is provided with a detection line 1, a detection line 2 and a control line, and the glass cellulose membrane of the first test strip is coated with a mass ratio of 1: 1 mixture of quantum dot-labeled adenovirus antibody and quantum dot-labeled rotavirus antibody; the detection line 1 of the first test strip is coated with adenovirus antigen, and the detection line 2 is coated with...

Embodiment 2

[0077] The kit for joint detection of four enteroviruses in this example differs from the example in that the quantum dots used are cadmium sulfide quantum dots, and the modified cyclodextrin used is aminated dry cyclodextrin. In the process of virus antibody, quantum dot-labeled rotavirus antibody, quantum dot-labeled astrovirus antibody, and quantum dot-labeled norovirus antibody, in the activated quantum dot-modified cyclodextrin-latex suspension The mixing time for adding the antibody or antigen is 2 hours, and the others are the same as in Example 1.

Embodiment 3

[0079] The kits for joint detection of four enteroviruses in this example are different from those in the examples in that adenovirus antibodies, quantum dot-labeled rotavirus antibodies, quantum dot-labeled astrovirus antibodies, and quantum dot-labeled Novavirus antibodies were prepared. For example, in the process of virus antibody, the mixing time for adding antibody or antigen to the activated quantum dot-modified cyclodextrin-latex suspension is 4 hours, and the others are the same as in Example 1.

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PUM

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Abstract

The invention relates to the technical field of immunochromatographic assay, and particularly discloses an immunochromatographic kit for joint detection of four enteroviruses and a preparation method for the immunochromatographic kit. The kit comprises a first test strip and a second test strip, wherein each of the first and second test strips comprises a plastic plate, a fiberglass membrane and a cellulose nitrate membrane; a detection line 1, a detection line 2 and a control line are arranged on the cellulose nitrate membrane; the fiberglass membrane of the first test strip is coated with a quantum dot-labeled adenovirus antibody and a quantum dot-labeled rotavims antibody; the detection line 1 of the first test strip is coated with an adenovirus antigen, and the detection line 2 is coated with a rotavims antigen; the fiberglass membrane of the second test strip is coated with a quantum dot-labeled astrovirus antibody and a quantum dot-labeled norovirus antibody; the detection line 1 of the second test strip is coated with an astrovirus antigen, and the detection line 2 is coated with a norovirus antigen. The kit can be used for implementing rapid and sensitive joint detection of the enteroviruses.

Description

technical field [0001] The invention relates to the technical field of immunochromatography detection, in particular to an immunochromatography kit for combined detection of four enteroviruses and a preparation method thereof. Background technique [0002] Rotavirus (rotavims, RV) is one of the main pathogens of acute diarrhea in humans and animals all over the world. Membrane, observed under the electron microscope after negative staining, the shape of the virus is wheel-shaped, hence the name. Lives in feces for days to weeks. It is resistant to ether, acid, alkali and repeated freezing and thawing, and has a wide pH range (pH3.5-10). It is relatively stable at room temperature and can be inactivated at 55°C for 30 minutes. The genome and its encoded protein are double-stranded RNA virus, about 18550bp, composed of 11 gene segments. Each fragment contains an open reading frame, encoding 6 structural proteins (VP1, VP2, VP3, VP4, VP6, VP7) and 5 nonstructural proteins (...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/558G01N33/56983
Inventor 金鑫曾滨赵正道
Owner BEIJING MOKOBIO LIFE SCI CO LTD
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