Hydrophobic ionic liquid fixed cholesterol enzyme/Prussian blue modified electrode for detecting cholesterol

An ionic liquid and cholesterol enzyme technology, applied in the field of biosensors, can solve the problems of poor cholesterol enzyme biocompatibility, unsatisfactory detection effect, and poor electrode stability, and achieves high sensitivity, simple detection process, The effect of increasing sensitivity

Inactive Publication Date: 2014-01-29
NORTHWEST NORMAL UNIVERSITY
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  • Abstract
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Problems solved by technology

[0006] Most of the existing cholesterol sensors use conductive polymers or composites made of nanomaterials as enzyme immobilization substrates. Good, and the detection effect is not particularly ideal

Method used

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  • Hydrophobic ionic liquid fixed cholesterol enzyme/Prussian blue modified electrode for detecting cholesterol
  • Hydrophobic ionic liquid fixed cholesterol enzyme/Prussian blue modified electrode for detecting cholesterol
  • Hydrophobic ionic liquid fixed cholesterol enzyme/Prussian blue modified electrode for detecting cholesterol

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Embodiment Construction

[0024] In order to illustrate content of the present invention more clearly, the present invention will be further described below in conjunction with accompanying drawing and specific embodiment:

[0025] Instruments or medicines used in the examples of the present invention: CHI660 electrochemical analyzer (Shanghai Chenhua Instrument Co., Ltd.) for cyclic voltammetry experiments; saturated calomel reference electrode (Shanghai Ridao Scientific Instrument Co., Ltd.); quartz tube Heated automatic double pure water distiller (1810B, Shanghai Asia Pacific Technology Glass Co., Ltd.) was used to prepare double distilled water; electronic balance (Beijing Sartorius Instrument Co., Ltd.) was used to weigh medicine; ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.) company); aluminum oxide grinding powder (0.30 μm, 0.05 μm, Shanghai Chenhua Instrument Reagent Co., Ltd.) was used to treat glassy carbon electrodes; Acyl salt (Shanghai Chengjie Chemical Co., Ltd.); Potassiu...

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Abstract

The invention discloses an ionic liquid film fixed cholesterol enzyme / Prussian blue-based modified electrode. A cholesterol oxidase is fixed on an electrode through ionic liquid to form a modified layer on the surface of the electrode, and concretely, the cholesterol oxidase is dispersed into hydrophobic ionic liquid to form a cholesterol enzyme ionic liquid stock solution, then, the solution is coated on the surface of the electrode on which Prussian blue is electrodeposited, and finally, the ionic liquid film fixed cholesterol enzyme / Prussian blue-based modified electrode is obtained after vacuum drying. The modified electrode disclosed by the invention can be directly used for detecting cholesterol and has the characteristics of high sensitivity and low detection limit.

Description

[0001] technical field [0002] The invention relates to a biosensor, in particular to a Prussian blue modified electrode for detecting cholesterol based on hydrophobic ionic liquid immobilized cholesterol enzyme. Background technique [0003] Biosensor (biosensor) is a special type of sensor, which is an instrument that is sensitive to biological substances and converts its concentration into electrical signals for detection. It consists of immobilized biologically sensitive materials (enzymes, antibodies, antigens, microorganisms, cells, tissues, nucleic acids and other biologically active substances) as identification elements and appropriate physical and chemical transducers (such as oxygen electrodes, photosensitive tubes, field effect tubes, Analytical tools or systems composed of piezoelectric crystals, etc.) and signal amplification devices, sensors using immobilized biological components or organisms as sensitive elements are called biosensors. [0004] Ionic liqui...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/327
Inventor 刘秀辉南志汉裘宇郑李纯卢小泉
Owner NORTHWEST NORMAL UNIVERSITY
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