40 results about "Concentration dependence" patented technology

The invention belongs to the technical field of functional material and fluorescent nano probes, and relates to a novel hairpin DNA template used for synthesis of fluorescent nano copper clusters, and applications of the novel hairpin DNA template in biomolecule detection. The novel hairpin DNA template possesses a hairpin structure; a loop part is composed of poly T with different length, and a stem part is composed of double-stranded DNA with different length and base sequences; and in a certain buffer solution, fluorescence signals can be generated via a few minutes of reaction at room temperature under the action of copper sulfate and vitamin C. Compares with linear poly T, the novel hairpin DNA template possesses following advantages: the fluorescence intensity of the formed fluorescent nano copper clusters is higher, and possesses poly T length and sequence concentration dependence; formation of the fluorescent nano copper clusters is influenced by the stability of the novel hairpin DNA template; formation conditions are mild; reaction is quick; fluorescence intensity is higher than that of the prior art; and biomolecule detection technology without labeling or separating can be designed easily.

The invention discloses a lanthanide-MOF (Metal Organic Framework) doping-based electrostatic spinning fiber membrane serving as a fluorescent sensor and a preparation method thereof. The sensor is applied to the quantitative detection of uric acid in human urine. The detection process comprises the following steps of: (I) synthesizing Eu-PTA-MOF powder; (II) synthesizing a copolymer PMMA/PVP; (III) preparing an electrostatic spinning solution; (IV) preparing a nanofiber membrane; (V) cutting the nanofiber membrane; (VI) inserting the cut membrane into a cuvette; and (VII) recording fluorescence spectrum data. The prepared electrostatic spinning fiber membrane shows various kinds of characteristic blue fluorescence of Eu<3+> and good hydrophilicity and shows good fluorescence detection stability at the pH value of 4.5-8.0 in 30 minutes; the quenching efficiency of the uric acid on the electrostatic spinning fiber membrane fluorescence shows good concentration dependence and anti-interference performance; the detection range of the uric acid is 3-100 [mu]M and the detection limit is 1.3 [mu]M; in addition, the electrostatic spinning fiber membrane can be used repeatedly. The invention provides a simple and efficient method for the quantitative detection of the uric acid in biological fluids.

The present invention provides a preparation method of angiogenesis inhibitory factor. Dasyatis cartilage is firstly crashed, then extracted, roughly separated and purified, finally desalinated, frozen and dried, thus obtaining the dasyatis cartilage angiogenesis inhibitory factor, shortened as DCAIF-I; wherein, the purification adopts Hitrap DEAEFF ion-exchange column chromatography, Superdex 75 10/300 GL ion-exchange column chromatography and Shim-packVP-ODS HPLC ion-exchange column chromatography. The preparation method of angiogenesis inhibitory factor provided by the present invention provides a method of extracting the angiogenesis inhibitory factor from the dasyatis cartilage. Through experiment, the DCAIF-I obtained in the method can effectively inhibit the angiogenesis and has a certain concentration dependence. The preparation method is a method in which the angiogenesis inhibitory factor can be effectively and optimally distilled from the dasyatis cartilage.

A new method is presented for measuring the molecular properties of an unfractionated solution of macromolecules. Sample aliquots spanning a range of concentrations are injected sequentially into a stream of solution and flow towards the detectors. Each aliquot produces, thereby, an effective “peak” whose elements correspond to different concentrations of the diluted aliquot. The weight averaged molar mass, the mean square radius, and the second virial coefficient of the macromolecules in solution are derived from an analysis of the angular and concentration dependence of the scattering signals throughout the corresponding peaks. In contrast to earlier on-line methods, better accuracy is achieved, while using a smaller quantity of sample. A similar method for determining cross virial coefficients between two distinct species of macromolecules is also presented.

The invention relates to a preparation method and application of an Eu<3+>-MOF material. The preparation method comprises the following steps: preparing an iron-based MOF material from ferrous chloride and pyromellitic acid and then preparing the Eu<3+>-MOF material from the prepared iron-based MOF material and europium nitrate. The prepared Eu<3+>-MOF material shows strong characteristic emissionlight of Eu<3+> and has good water stability and thermal stability. Ag<+> has good concentration dependence on the enhancement efficiency of Eu<3+>-MOF fluorescence. The detection range of Ag<+> is 0-150 [mu]M, the detection limit is 0.09 [mu]M, and the response speed of the material to Ag<+> is very fast (3 min).

The invention discloses a blueberry lip balm. The blueberry lip balm comprises the following components in parts by weight: 0.025-0.1 part of blueberry anthocyanin, 0.2-0.5 part of a lipstick toner, 2-3 parts of sweet almond oil, 0.8-1.2 parts of beewax, 0.3-0.7 part of shea butter and 0.5-1 part of water. Through test, an obvious concentration dependence relation exists between a peroxide value of the lip balm and the adding quantity of the anthocyanin, and the more the adding quantity of the anthocyanin is, the stronger the antioxidant ability of the lip balm is; the adding quantity of the anthocyanin has no significant influence on the peroxide value, the hardness and the deformation quantity; by the use of the anthocyanin, the viscosity and the elasticity of the lip balm can also be reduced; moreover, the adding quantity of the anthocyanin has a certain influence on color difference. In conclusion, the anthocyanin cannot be blindly added so as to pursue the oxidation resistance of the anthocyanin. Although the anthocyanin is good, the anthocyanin is also required to be added in moderation; when the anthocyanin of 0.05-0.075 g is added in the lip balm per tube, the color effect of the lip balm is better.

The invention discloses application of amide compounds to the preparation of quorum sensing activity inhibitors. Oceanobacillus sp. XC22919 fermentation broth is utilized for separation and purification to prepare compounds 2-methyl-N-(2'-phenethyl)butyramide and 3-methyl-N-(2'-phenethyl)butyramide, which do not inhibit the growth of chromobacterium violaceum CV026 within a concentration range from 0Mu g/mL to 70Mu g/mL, but can remarkably reduce the generation of chromobacterium violaceum purpurin; moreover, as concentration is gradually increased, the effect of inhibiting the generation of purpurin is higher; within a concentration range from 0Mu g/mL to 100Mu g/mL, the compounds do not affect the growth of pseudomonas aeruginosa PAO1, but can remarkably decrease the expression of the virulence factor of pseudomonas aeruginosa, and can remarkably decrease the expressions of virulence factors such as pyocyanin, elastase, proteolytic enzyme and organism membranes, and the effect of the compounds in inhibiting quorum sensing is concentration-dependence.

Organophosphorus pesticides (OPs) are highly favored due to effects of high efficiency and wide action and are normally used as agricultural pesticides and herbicides, while a series of serious healthproblems, such as dizziness, vomiting, diarrhea and muscle tremor even death, can be caused to a human body due to OPs residue, and thus, a real-time quantitative detection method for OPs is urgentlyneeded to be designed and developed. Here, a visual colorimetric detection technology is built for the organophosphorus pesticides. According to the visual colorimetric detection technology, triazophos is selected as an organophosphorus pesticide representative model molecule, acetylthiocholine iodide (ATCI) is used as a acetylcholinesterase (AchE) catalytic substrate, the ATCI can be catalyzed and decomposed to sulfhydryl choline, and I2-induced gold nanorod etching can be prevented by reduction sulfhydryl at a tail end; when OPs exist, the activity of the AchE is suppressed, no reduction sulfhydryl is generated, I<-> can be oxidized by KIO3 to generate I2, etching reaction of the gold nanorod is finally triggered by the I2, the gold nanorod with OPs concentration dependence longitudinally absorbs peak location blue shift, and color change of a gold nanorod solution is caused. By the novel method, favorable performance actual application ability of organophosphorus pesticide detection can be verified, and the method has obvious creativity and application value.

The invention discloses application of 3,5-di-tert-butyl copper salicylate to preparing drug for treating high-metastatic breast cancer and administration dosage of 3,5-di-tert-butyl copper salicylate. Experiments prove that 3,5-di-tert-butyl copper salicylate can inhibit transfer of breast cancer cells, and breast cancer cell invasion is inhibited through concentration dependence. New medical application of 3,5-di-tert-butyl copper salicylate is developed, and a new application field is exploited.

The present invention relates to, among other things, methods and systems for recognizing and characterizing protein aggregation processes at the earliest possible time and use of such new methods and systems for (1) the identification and selection of protein formulations that minimize aggregation and extend long-term stability and (2) the identification of protein variants with the lowest tendency to aggregate.

The invention relates to a preparation method of a material for detecting the content of dopamine in a human body. The preparation method comprises the following steps: firstly, preparing a Zr-based metal organic framework of which the chemical composition is Zr6O4(OH)4(O2C-C6H2-CO2(CO2H)2).XH2O; and then synthesizing a Tb<3+>-MOF material by utilizing the Zr-based metal organic framework. The prepared Tb<3+>-MOF material shows strong characteristic emission fluorescence of Tb<3+> and good water resistance. The Tb<3+>-MOF material maintains good stability in a pH range of 3.0-10.0, and still maintains a complete crystal structure and fluorescence characteristics after being soaked in an aqueous solution environment for 48h. DA has good concentration dependence and anti-interference performance on the quenching efficiency of fluorescence of the Tb<3+>-MOF material. The detection range of DA is 0-350 [mu] M, and the detection limit is 0.06 [mu] M. In addition, the Tb<3+>-MOF material canbe repeatedly used, and portable test paper is manufactured to be applied to an actual physiological environment. The invention provides a simple and efficient method for detecting the DA in the biological fluid.

The invention belongs to the technical field of protein aggregation inhibition, and concretely relates to a probe molecule specifically bind to a pathological marker Tau protein in Alzheimer disease and capable of inhibiting Tau protein aggregation. The probe molecule specifically binds to the Tau protein and is capable of inhibiting Tau protein aggregation, and the inhibition effect presents concentration dependence. The probe molecule has the advantages that by adjusting length of intermediate ether chain, affinity to a Tau protein monomer and aggregation is realized, and the probe molecule has great influence on the inhibition capability of the Tau protein aggregation by the length of the ether chain.

The present invention relates to a Raoultella sp. pan22x and application thereof to production of a bacteriostatic agent for inhibiting purple pigment generation. The marine Raoultella sp. pan22x is preserved in China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, No 3, Yard No.1, Beichen West Road, Chaoyang District, Beijing (zip code 100101) on the date of March 11, 2016, and has an accession number CGMCC NO. 12208. The bacteriostatic agent of the invention can effectively inhibit purple pigment production of C.violaceum ATCC12472 and C. violaceum CV026 in the sample addition amount of 0-2.0mg. The antibacterial agent has quorum sensing inhibition effect showing concentration dependence; along with the increase of sample addition amount, the inhibition effect is enhanced; in concentration range of 0.1-0.3mg / mL, the inhibition rate of purple pigment production of C. violaceum ATCC 12472 reaches 54% without affecting cell growth. The Raoultella sp. pan22x fermentation productscan be used to research on the prevention of purple bacterium infections, and the development of new antimicrobial agents in the medical field.

The invention discloses a toner and a preparation method thereof and belongs to the field of skincare products. Active substance in sargassum is extracted through fermentation, and fucoxanthin is coated by a nano emulsion, so that storage stability of fucoxanthin is improved; acne bacteria exist on skin follicles and generate lipase during growing, and the lipase causes a series of inflammatory reaction including migration of white blood cells to skin corium layer and release of inflammatory factors which stimulate skin and accelerate skin cornification so as to generate and deteriorate acne;sargassum extract has inhibiting effect on activation of the lipase of the acne bacteria and has concentration dependence, thereby realizing acne-resisting and inflammation-diminishing effect. By thetoner, the problem that most existing toner contains ingredients like perfume, alcohol and heavy preservative, thereby being prone to causing burden on thin skin and skin prone to having sensitive reaction to external stimulation, unitary in nutritional ingredient, lacking in nourishing ingredient and short in moisturizing effect lasting time is solved.

The invention discloses application of artemisinin in the preparation of drugs to treat c-myc/mTOR-activated human hepatoma SMMC7721 cells. Artemisinin is a colorless needle crystal of sesquiterpene lactone with peroxyl groups, extracted from stems and leaves of the compound inflorescence plant Artemisia annua. In the invention, it is discovered for the first time that artemisinin can inhibit theproliferation of human hepatoma SMMC7721 cells, with good time and concentration dependence presented, can induce apoptosis of the human hepatoma SMMC7721 cells by inhibiting the expression of P-c-mycand P-S6 of c-myc/mTOR pathway, and therefore, can be studied and developed as a candidate drug to induce the apoptosis of human hepatoma SMMC7721 cells.

The invention provides an antitumor drug and a preparation method and application thereof. The antitumor drug has the structure as shown as the chemical compound D in the formula I, can effectively inhibit growth of tumor cells and increase weight of mice. Besides, effect of the drug in high dose is better than that of the drug in middle dose, and the effect of the drug in middle dose is better than that of the drug in low dose, in other words, the antitumor drug has concentration dependence. Further, the preparation method of the antitumor drug is simple and easy to operate and can effectively reduce production cost to realize industrialized production of the antitumor drug.

A new method is presented for characterizing the associative properties of a solution of macromolecules at high concentration. Sample aliquots spanning a range of concentrations are injected sequentially into a light scattering photometer. Equilibrium association constants and association stoichiometry are derived from an analysis of the angular and concentration dependence of the scattering signals. Thermodynamic nonideality, which becomes important at high concentrations, is dealt with in the analysis in a simplified manner which is applicable to multiple associated species.

The invention discloses an application of a zinc salicylate dihydrate in preparing a medicine for treating a negative breast cancer containing estrogen receptors and an administration dosage of the zinc salicylate dehydrate. The experiment proves that the zinc salicylate dihydrate is capable of inhibiting proliferation and migration of breast cancer cells so as to promote the apoptosis of the breast cancer cells in a concentration dependence manner. Thus, a novel medical application of the zinc salicylate dehydrate is explored, i.e., a novel application field is exploited.