6 results about "Concentration dependence" patented technology

The invention provides an intelligent veterinary drug residue detection system and method based on multi-dimensional spectral characteristics, and relates to the technical field of veterinary drug residue detection, spectral response data of a to-be-detected sample solution in a visible light band and a near-infrared band is collected, and the residue discrimination degree of veterinary drug residues under any wavelength when the concentration dependence change exists is determined; abnormal wave band combinations with quantitative response to veterinary drug residues are screened out from the visible light wave band and the near-infrared wave band respectively, the residual peak deviation of the veterinary drug residues in the region of interest of the spectrum is determined according to the abnormal wave band combinations, and trace residue marks in the multiple spectrum intensity distribution modes are determined; and performing in-situ nondestructive screening on veterinary drug residues in the to-be-detected animal-derived food sample according to the residue discrimination and the trace residue mark. According to the method, on the premise that the dual-band spectrum collaborative response is enhanced, the veterinary drug residue characteristics can be directionally distinguished and detected, so that the veterinary drug residue screening accuracy is improved.

The invention relates to a drug effect evaluation method for fusing target molecules and time-concentration dependent cell phenotypes, which comprises the following steps: S1, preparing samples including a modeling sample and a to-be-detected sample; s2, carrying out FRET imaging and cell fluorescence imaging; s3, carrying out FRET image processing and FRET efficiency calculation; s4, cell fluorescence image processing and feature extraction; s5, calculating a phenotype characterization value; s6, calculating an FRET characterization value; s7, basic drug response value calculation; and S8, comprehensive drug effect evaluation. Target molecule information and time-concentration dependent cell phenotypic response are fused, a comprehensive drug effect evaluation model is constructed, the effect of drugs on whole cells is reflected, and whether the drugs target specified molecules or not is specifically indicated.

The method according to the invention (200) serves to determine the concentration of a component of a binary mixture from a set of binary mixtures that differ from each other in at least one component; wherein a first measurand has specific concentration dependencies for the components of the set of binary mixtures; wherein at least one second measurand for the different mixtures of the set of binary mixtures each has specific dependencies on the first measurand; wherein the method comprises: determining a first measured value of the first measurand for the binary mixture (210); determining at least one second measured value of the second measurand for the binary mixture (220); identifying the concentration dependency of the first measurand valid for the mixture based on the first measured value of the first measurand and the at least one second measured value of the second measurand (230);and determining the concentration of the component based on the first measurement and the valid concentration dependence (240).;

The application discloses a dual-response fluorescent probe and a preparation method and detection application thereof, and belongs to the technical field of fluorescent probes. The probe is prepared by condensation reaction of 4-methyl-7-hydroxy-8-aldehyde coumarin and 4-morpholine aniline, and can simultaneously and specifically respond to peroxynitrite and viscosity. ‑ The probe specifically reacts with ONOO to generate a concentration-dependent fluorescence enhancement, has high selectivity, fast response and good linearity, and the fluorescence is significantly enhanced with the increase of viscosity. The probe has lysosome targeting properties, and can realize real-time imaging and dynamic monitoring of endogenous and exogenous ONOO ‑ in living cells, and presents a high-contrast fluorescent signal in a non-alcoholic fatty liver model mouse liver tissue slice, so that ONOO ‑ overproduction and viscosity increase in a pathological state can be accurately identified.

The invention discloses a rapid and sensitive SERS (Surface Enhanced Raman Scattering) detection method for GPH (Glyphosate Phosphate), which comprises the following steps: S1, preparing an SERS substrate, namely boiling AgNO3 and Na3-citrate solutions, and reacting until the solution is grey green to obtain an AgNPs solution; s2, performing a first-step derivatization reaction: adding a DMSO solution containing CS2 into 0.3-1.5 mL of a GPH solution, performing a reaction for 6-16 min, adding 10-30 [mu] L of 1 M NaOH, and performing a reaction for 3-6 min to obtain a reaction product A; s3, second-step derivatization reaction: adding the product A into an SERS (Surface Enhanced Raman Scattering) substrate, adding 120-180 [mu] L of 0.1 M HNO3, and uniformly mixing to obtain a reaction product B; and S4, performing SERS analysis: adding an aggregation inducer into the product B, and performing SERS analysis. According to the method disclosed by the invention, the amplification of the SERS signal of the final derivatization product is realized through the CS2-mediated two-step glyphosate derivatization reaction, so that the SERS signal intensity of the reaction system has GPH concentration dependence. The GPH detection time is shortened, the detection efficiency is greatly improved on the basis that high sensitivity of a traditional method is reserved, and the method is easy to operate and small in reagent consumption.

This invention discloses a method for synthesizing and applying an enhanced fluorescent probe that specifically recognizes mercury ions. The probe is based on the rhodamine structure and modified with a pyridyl isothiocyanate structure, with the molecular formula C0.05. 34 H 35 N6O2S, labeled FS-Hg, is a fluorescent probe that can monitor mercury ions in real time in N,N-dimethylformamide solution. It only causes a visible color change (from colorless to bright pink) and new spectral bands upon recognition of mercury ions. The probe also causes a sharp increase in absorbance at 568 nm and a sharp increase in fluorescence intensity at 594 nm upon mercury ion recognition. The mercury ion recognition process exhibits concentration dependence, extremely high sensitivity, good selectivity, and stable sensing characteristics. This probe can efficiently detect mercury ion concentration in aquatic products, which is of great significance for on-site detection and large-scale screening of heavy metals in food safety.