2 results about "Concentration dependence" patented technology

The method according to the invention (200) serves to determine the concentration of a component of a binary mixture from a set of binary mixtures that differ from each other in at least one component; wherein a first measurand has specific concentration dependencies for the components of the set of binary mixtures; wherein at least one second measurand for the different mixtures of the set of binary mixtures each has specific dependencies on the first measurand; wherein the method comprises: determining a first measured value of the first measurand for the binary mixture (210); determining at least one second measured value of the second measurand for the binary mixture (220); identifying the concentration dependency of the first measurand valid for the mixture based on the first measured value of the first measurand and the at least one second measured value of the second measurand (230);and determining the concentration of the component based on the first measurement and the valid concentration dependence (240).;

The application discloses a dual-response fluorescent probe and a preparation method and detection application thereof, and belongs to the technical field of fluorescent probes. The probe is prepared by condensation reaction of 4-methyl-7-hydroxy-8-aldehyde coumarin and 4-morpholine aniline, and can simultaneously and specifically respond to peroxynitrite and viscosity. ‑ The probe specifically reacts with ONOO to generate a concentration-dependent fluorescence enhancement, has high selectivity, fast response and good linearity, and the fluorescence is significantly enhanced with the increase of viscosity. The probe has lysosome targeting properties, and can realize real-time imaging and dynamic monitoring of endogenous and exogenous ONOO ‑ in living cells, and presents a high-contrast fluorescent signal in a non-alcoholic fatty liver model mouse liver tissue slice, so that ONOO ‑ overproduction and viscosity increase in a pathological state can be accurately identified.