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40 results about "Concentration dependence" patented technology
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Concentration Dependence. As more solvent diffuses into a polymer the structure opens up, making it even easier for solvent to diffuse - in other words the diffusion coefficient is concentration dependent.
Hairpin DNA template of fluorescent nano copper clusters, and applications thereof
InactiveCN106282175AFormation conditions are mildRapid responseMicrobiological testing/measurementDNA/RNA fragmentationRoom temperatureNano copper
The invention belongs to the technical field of functional material and fluorescent nano probes, and relates to a novel hairpin DNA template used for synthesis of fluorescent nano copper clusters, and applications of the novel hairpin DNA template in biomolecule detection. The novel hairpin DNA template possesses a hairpin structure; a loop part is composed of poly T with different length, and a stem part is composed of double-stranded DNA with different length and base sequences; and in a certain buffer solution, fluorescence signals can be generated via a few minutes of reaction at room temperature under the action of copper sulfate and vitamin C. Compares with linear poly T, the novel hairpin DNA template possesses following advantages: the fluorescence intensity of the formed fluorescent nano copper clusters is higher, and possesses poly T length and sequence concentration dependence; formation of the fluorescent nano copper clusters is influenced by the stability of the novel hairpin DNA template; formation conditions are mild; reaction is quick; fluorescence intensity is higher than that of the prior art; and biomolecule detection technology without labeling or separating can be designed easily.
Owner:FUDAN UNIV
Lanthanide-MOF (Metal Organic Framework) doping-based electrostatic spinning fiber membrane serving as fluorescent sensor for selectively detecting uric acid and preparation method thereof
ActiveCN109283164ASimple dilutionWide pH detection rangeMonocomponent synthetic polymer artificial filamentFluorescence/phosphorescenceFiberMetal-organic framework
The invention discloses a lanthanide-MOF (Metal Organic Framework) doping-based electrostatic spinning fiber membrane serving as a fluorescent sensor and a preparation method thereof. The sensor is applied to the quantitative detection of uric acid in human urine. The detection process comprises the following steps of: (I) synthesizing Eu-PTA-MOF powder; (II) synthesizing a copolymer PMMA / PVP; (III) preparing an electrostatic spinning solution; (IV) preparing a nanofiber membrane; (V) cutting the nanofiber membrane; (VI) inserting the cut membrane into a cuvette; and (VII) recording fluorescence spectrum data. The prepared electrostatic spinning fiber membrane shows various kinds of characteristic blue fluorescence of Eu<3+> and good hydrophilicity and shows good fluorescence detection stability at the pH value of 4.5-8.0 in 30 minutes; the quenching efficiency of the uric acid on the electrostatic spinning fiber membrane fluorescence shows good concentration dependence and anti-interference performance; the detection range of the uric acid is 3-100 [mu]M and the detection limit is 1.3 [mu]M; in addition, the electrostatic spinning fiber membrane can be used repeatedly. The invention provides a simple and efficient method for the quantitative detection of the uric acid in biological fluids.
Owner:WUHAN DGENSEE CO LTD
Method for preparing angiogenesis inhibition factor
InactiveCN101200496ASuppress generationGenerate efficientlyFish material medical ingredientsGrowth factors/regulatorsDasyatisAngiogenesis Inhibition
The present invention provides a preparation method of angiogenesis inhibitory factor. Dasyatis cartilage is firstly crashed, then extracted, roughly separated and purified, finally desalinated, frozen and dried, thus obtaining the dasyatis cartilage angiogenesis inhibitory factor, shortened as DCAIF-I; wherein, the purification adopts Hitrap DEAEFF ion-exchange column chromatography, Superdex 75 10 / 300 GL ion-exchange column chromatography and Shim-packVP-ODS HPLC ion-exchange column chromatography. The preparation method of angiogenesis inhibitory factor provided by the present invention provides a method of extracting the angiogenesis inhibitory factor from the dasyatis cartilage. Through experiment, the DCAIF-I obtained in the method can effectively inhibit the angiogenesis and has a certain concentration dependence. The preparation method is a method in which the angiogenesis inhibitory factor can be effectively and optimally distilled from the dasyatis cartilage.
Owner:ZHEJIANG OCEAN UNIV
Application of benzyl benzoate in preparation of bacterial colony induction activity inhibitors
ActiveCN107630048AStable outputDoes not inhibit growthAntibacterial agentsOrganic active ingredientsGradual increaseVirulence factor
The invention discloses an application of benzyl benzoate in preparation of bacterial colony induction activity inhibitors; in the method, an oceanobacillus sp. XC22919 fermented product is used for separation and extraction, and in a concentration range of 0-60 [mu]g / mL, bacterial growth of chromobacterium violaceum CV026 is not inhibited, but generation of chromobacterium violaceum purpurin canbe significantly decreased; and with gradual increase of the concentration, the effect of inhibiting generation of the purpurin is stronger; the effect of the compound in inhibiting colony induction is in a concentration dependence. In a concentration range of 0-100 [mu]g / mL, bacterial growth of pseudomonas aeruginosa PAO1 is not affected, but the expression of virulence factors of pseudomonas aeruginosa can be significantly reduced, the expression of pyocyanin, elastase, proteinase, biological body membranes and other virulence factors can be significantly reduced, and the effect of the compound in inhibiting colony induction is in the concentration dependence.
Owner:ZHEJIANG UNIV OF TECH
Method for determining average properties of molecules in solution by injection into a flowing solvent
ActiveUS20090222219A1Improve automationImprove accuracyIon-exchange process apparatusAnalysis using chemical indicatorsMean squareSolvent
A new method is presented for measuring the molecular properties of an unfractionated solution of macromolecules. Sample aliquots spanning a range of concentrations are injected sequentially into a stream of solution and flow towards the detectors. Each aliquot produces, thereby, an effective “peak” whose elements correspond to different concentrations of the diluted aliquot. The weight averaged molar mass, the mean square radius, and the second virial coefficient of the macromolecules in solution are derived from an analysis of the angular and concentration dependence of the scattering signals throughout the corresponding peaks. In contrast to earlier on-line methods, better accuracy is achieved, while using a smaller quantity of sample. A similar method for determining cross virial coefficients between two distinct species of macromolecules is also presented.
Owner:WYATT TECH
Preparation method and application of Eu<3+>-MOF material
InactiveCN110938215AHigh selectivityHigh sensitivityFluorescence/phosphorescenceLuminescent compositionsFluorescenceWater chlorination
The invention relates to a preparation method and application of an Eu<3+>-MOF material. The preparation method comprises the following steps: preparing an iron-based MOF material from ferrous chloride and pyromellitic acid and then preparing the Eu<3+>-MOF material from the prepared iron-based MOF material and europium nitrate. The prepared Eu<3+>-MOF material shows strong characteristic emissionlight of Eu<3+> and has good water stability and thermal stability. Ag<+> has good concentration dependence on the enhancement efficiency of Eu<3+>-MOF fluorescence. The detection range of Ag<+> is 0-150 [mu]M, the detection limit is 0.09 [mu]M, and the response speed of the material to Ag<+> is very fast (3 min).
Owner:YANTAI UNIV
Blueberry lip balm and making method thereof
ActiveCN107456398AImprove antioxidant capacityIncrease stickinessCosmetic preparationsToilet preparationsColor effectViscosity
The invention discloses a blueberry lip balm. The blueberry lip balm comprises the following components in parts by weight: 0.025-0.1 part of blueberry anthocyanin, 0.2-0.5 part of a lipstick toner, 2-3 parts of sweet almond oil, 0.8-1.2 parts of beewax, 0.3-0.7 part of shea butter and 0.5-1 part of water. Through test, an obvious concentration dependence relation exists between a peroxide value of the lip balm and the adding quantity of the anthocyanin, and the more the adding quantity of the anthocyanin is, the stronger the antioxidant ability of the lip balm is; the adding quantity of the anthocyanin has no significant influence on the peroxide value, the hardness and the deformation quantity; by the use of the anthocyanin, the viscosity and the elasticity of the lip balm can also be reduced; moreover, the adding quantity of the anthocyanin has a certain influence on color difference. In conclusion, the anthocyanin cannot be blindly added so as to pursue the oxidation resistance of the anthocyanin. Although the anthocyanin is good, the anthocyanin is also required to be added in moderation; when the anthocyanin of 0.05-0.075 g is added in the lip balm per tube, the color effect of the lip balm is better.
Owner:SHENYANG AGRI UNIV
Application of indacaterol maleate to preparation of tumor resisting medicines
InactiveCN110013481AGrowth inhibitionAntiproliferative activityOrganic active ingredientsAntineoplastic agentsIndacaterolSide effect
The invention discloses an application of indacaterol maleate to preparation of tumor resisting medicines. According to the application of the invention, the situation that the indacaterol maleate canrestrain the growth of colorectal cancer is found for the first time. The indacaterol maleate can restrain the reproductive activity of colorectal cancer cells, block the cell cycle of the colorectalcancer, cause G1-period blocking to restrain tumor growth of in vivo colorectal cancer. Besides, the effect of the indacaterol maleate for restraining the cell proliferation activity of the colorectal cancer is more significant along with the increase of the concentration of the indacaterol maleate and prolonging of medication time, and the indacaterol maleate has concentration dependence on thecycle checking effect and in vivo growth of colorectal cancer cells. Besides, the indacaterol maleate is a medicine which is approved by FDA for sale, is lower in cost than developing new medicines, and does not have any obvious toxic or side effects, and when the indacaterol maleate is used for auxiliary treatment of the colorectal cancer, a new medicine source can be provided for auxiliary treatment of cancer.
Owner:JINAN UNIVERSITY
Application of amide compounds to preparation of quorum sensing activity inhibitors
InactiveCN107473980ASimple preparation processControllable fermentation conditionsBiological material analysisMicroorganism based processesOrganismVirulence factor
The invention discloses application of amide compounds to the preparation of quorum sensing activity inhibitors. Oceanobacillus sp. XC22919 fermentation broth is utilized for separation and purification to prepare compounds 2-methyl-N-(2'-phenethyl)butyramide and 3-methyl-N-(2'-phenethyl)butyramide, which do not inhibit the growth of chromobacterium violaceum CV026 within a concentration range from 0Mu g / mL to 70Mu g / mL, but can remarkably reduce the generation of chromobacterium violaceum purpurin; moreover, as concentration is gradually increased, the effect of inhibiting the generation of purpurin is higher; within a concentration range from 0Mu g / mL to 100Mu g / mL, the compounds do not affect the growth of pseudomonas aeruginosa PAO1, but can remarkably decrease the expression of the virulence factor of pseudomonas aeruginosa, and can remarkably decrease the expressions of virulence factors such as pyocyanin, elastase, proteolytic enzyme and organism membranes, and the effect of the compounds in inhibiting quorum sensing is concentration-dependence.
Owner:ZHEJIANG UNIV OF TECH
Visual colorimetric detection method of organophosphorus pesticides
ActiveCN110632062AAchieve semi-quantitative detectionRealize visual detectionMaterial analysis by observing effect on chemical indicatorMicrobiological testing/measurementEtchingAcetylcholine Iodide
Organophosphorus pesticides (OPs) are highly favored due to effects of high efficiency and wide action and are normally used as agricultural pesticides and herbicides, while a series of serious healthproblems, such as dizziness, vomiting, diarrhea and muscle tremor even death, can be caused to a human body due to OPs residue, and thus, a real-time quantitative detection method for OPs is urgentlyneeded to be designed and developed. Here, a visual colorimetric detection technology is built for the organophosphorus pesticides. According to the visual colorimetric detection technology, triazophos is selected as an organophosphorus pesticide representative model molecule, acetylthiocholine iodide (ATCI) is used as a acetylcholinesterase (AchE) catalytic substrate, the ATCI can be catalyzed and decomposed to sulfhydryl choline, and I2-induced gold nanorod etching can be prevented by reduction sulfhydryl at a tail end; when OPs exist, the activity of the AchE is suppressed, no reduction sulfhydryl is generated, I<-> can be oxidized by KIO3 to generate I2, etching reaction of the gold nanorod is finally triggered by the I2, the gold nanorod with OPs concentration dependence longitudinally absorbs peak location blue shift, and color change of a gold nanorod solution is caused. By the novel method, favorable performance actual application ability of organophosphorus pesticide detection can be verified, and the method has obvious creativity and application value.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
Application of 3,5-di-tert-butyl copper salicylate to preparing drug for treating high-metastatic breast cancer
ActiveCN105412125AWide variety of sourcesHigh puritySalicyclic acid active ingredientsAntineoplastic agentsGynecologyTert butyl
The invention discloses application of 3,5-di-tert-butyl copper salicylate to preparing drug for treating high-metastatic breast cancer and administration dosage of 3,5-di-tert-butyl copper salicylate. Experiments prove that 3,5-di-tert-butyl copper salicylate can inhibit transfer of breast cancer cells, and breast cancer cell invasion is inhibited through concentration dependence. New medical application of 3,5-di-tert-butyl copper salicylate is developed, and a new application field is exploited.
Owner:JIANGHAN UNIVERSITY
Determination of Protein Aggregation from the Concentration Dependence of Delta G
InactiveUS20160018406A1Minimizing aggregationImprove long-term stabilityLibrary screeningBiological testingCell AggregationsProtein aggregation
The present invention relates to, among other things, methods and systems for recognizing and characterizing protein aggregation processes at the earliest possible time and use of such new methods and systems for (1) the identification and selection of protein formulations that minimize aggregation and extend long-term stability and (2) the identification of protein variants with the lowest tendency to aggregate.
Owner:UNCHAINED LABS
A kind of carbendazim detection method based on fluorescent probe
The invention provides a carbendazim detection method based on a fluorescent probe, comprising the following steps: dissolving the fluorescent probe with an organic solvent to prepare a solution A; preparing an alkali solution B; preparing a carbendazim standard solution, adding Solution A and solution B, detect the fluorescence signal intensity, draw the standard curve of the relationship between the fluorescence intensity and the concentration of the carbendazim standard solution; prepare the sample solution to be tested, add solution A and solution B to it, detect the fluorescence signal intensity, according to the obtained The content of carbendazim in the samples was obtained from the standard curve. The present invention generates a fluorescent signal through the specific reaction between the fluorescent probe and the carbendazim, and determines the content of the carbendazim in the sample according to the relationship between the fluorescent signal intensity and the concentration by detecting the intensity of the fluorescent signal, the method is simple, the cost is low, and the The detection of carbendazim content under alkaline conditions has the characteristics of high specificity, high sensitivity, accuracy and reliability, and can be used for rapid detection of carbendazim content in fruits and vegetables.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI
Method for determining average properties of molecules in solution
ActiveUS7813882B2Improve accuracyReduce effortIon-exchange process apparatusAnalysis using chemical indicatorsMean squareMolecular property
A new method is presented for measuring the molecular properties of an unfractionated solution of macromolecules. Sample aliquots spanning a range of concentrations are injected sequentially into a stream of solution and flow towards the detectors. Each aliquot produces, thereby, an effective “peak” whose elements correspond to different concentrations of the diluted aliquot. The weight averaged molar mass, the mean square radius, and the second virial coefficient of the macromolecules in solution are derived from an analysis of the angular and concentration dependence of the scattering signals throughout the corresponding peaks. In contrast to earlier on-line methods, better accuracy is achieved, while using a smaller quantity of sample. A similar method for determining cross virial coefficients between two distinct species of macromolecules is also presented.
Owner:WYATT TECH LLC
Preparation method of material for detecting content of dopamine in human body
InactiveCN111100304AImprove structural stabilityStrong fluorescenceFluorescence/phosphorescenceLuminescent compositionsHuman bodyMetal-organic framework
The invention relates to a preparation method of a material for detecting the content of dopamine in a human body. The preparation method comprises the following steps: firstly, preparing a Zr-based metal organic framework of which the chemical composition is Zr6O4(OH)4(O2C-C6H2-CO2(CO2H)2).XH2O; and then synthesizing a Tb<3+>-MOF material by utilizing the Zr-based metal organic framework. The prepared Tb<3+>-MOF material shows strong characteristic emission fluorescence of Tb<3+> and good water resistance. The Tb<3+>-MOF material maintains good stability in a pH range of 3.0-10.0, and still maintains a complete crystal structure and fluorescence characteristics after being soaked in an aqueous solution environment for 48h. DA has good concentration dependence and anti-interference performance on the quenching efficiency of fluorescence of the Tb<3+>-MOF material. The detection range of DA is 0-350 [mu] M, and the detection limit is 0.06 [mu] M. In addition, the Tb<3+>-MOF material canbe repeatedly used, and portable test paper is manufactured to be applied to an actual physiological environment. The invention provides a simple and efficient method for detecting the DA in the biological fluid.
Owner:YANTAI UNIV
Molecule specifically bind to Tau protein and capable of inhibiting Tau protein aggregation, preparation method and application thereof
The invention belongs to the technical field of protein aggregation inhibition, and concretely relates to a probe molecule specifically bind to a pathological marker Tau protein in Alzheimer disease and capable of inhibiting Tau protein aggregation. The probe molecule specifically binds to the Tau protein and is capable of inhibiting Tau protein aggregation, and the inhibition effect presents concentration dependence. The probe molecule has the advantages that by adjusting length of intermediate ether chain, affinity to a Tau protein monomer and aggregation is realized, and the probe molecule has great influence on the inhibition capability of the Tau protein aggregation by the length of the ether chain.
Owner:FUDAN UNIV
Preparation method of pH-concentration dependent tertiary amine chromophore polymer
ActiveCN110845725ANo irritating smellReduce energy consumptionLuminescent compositionsPolymer scienceDrug carrier
The invention belongs to the technical field of responsive fluorescence, and specifically discloses a preparation method of a pH-concentration dependent tertiary amine chromophore polymer. According to the preparation method, tertiary amine containing hydroxyl monomers and unsaturated acrylate double-bond monomers carry out Oxa-Michael addition solution polymerization to simply prepare a polymer,whose molecular skeleton comprises a tertiary amine structure, in one step. The preparation method does not need a step of preparing monomers, the reaction conditions are mild, the post treatment is simple, and the preparation method can be applied to large scale production. Furthermore, the prepared polymer containing a tertiary amine structure has good water solubility and bio-compatibility, ispH-concentration dependent, and has a great research value and application prospect in fields of bio-detection and drug carriers.
Owner:CHANGZHOU UNIV
Raoultella sp. pan22x and application thereof
ActiveCN105861356ASimple fermentation conditionsInhibitionAntibacterial agentsBacteriaFermentationAntibacterial agent
The present invention relates to a Raoultella sp. pan22x and application thereof to production of a bacteriostatic agent for inhibiting purple pigment generation. The marine Raoultella sp. pan22x is preserved in China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, No 3, Yard No.1, Beichen West Road, Chaoyang District, Beijing (zip code 100101) on the date of March 11, 2016, and has an accession number CGMCC NO. 12208. The bacteriostatic agent of the invention can effectively inhibit purple pigment production of C.violaceum ATCC12472 and C. violaceum CV026 in the sample addition amount of 0-2.0mg. The antibacterial agent has quorum sensing inhibition effect showing concentration dependence; along with the increase of sample addition amount, the inhibition effect is enhanced; in concentration range of 0.1-0.3mg / mL, the inhibition rate of purple pigment production of C. violaceum ATCC 12472 reaches 54% without affecting cell growth. The Raoultella sp. pan22x fermentation productscan be used to research on the prevention of purple bacterium infections, and the development of new antimicrobial agents in the medical field.
Owner:ZHEJIANG UNIV OF TECH
Toner and preparation method thereof
InactiveCN107951812ACan regulate endocrineGood moisture absorptionCosmetic preparationsToilet preparationsFucoxanthinInflammatory factors
The invention discloses a toner and a preparation method thereof and belongs to the field of skincare products. Active substance in sargassum is extracted through fermentation, and fucoxanthin is coated by a nano emulsion, so that storage stability of fucoxanthin is improved; acne bacteria exist on skin follicles and generate lipase during growing, and the lipase causes a series of inflammatory reaction including migration of white blood cells to skin corium layer and release of inflammatory factors which stimulate skin and accelerate skin cornification so as to generate and deteriorate acne;sargassum extract has inhibiting effect on activation of the lipase of the acne bacteria and has concentration dependence, thereby realizing acne-resisting and inflammation-diminishing effect. By thetoner, the problem that most existing toner contains ingredients like perfume, alcohol and heavy preservative, thereby being prone to causing burden on thin skin and skin prone to having sensitive reaction to external stimulation, unitary in nutritional ingredient, lacking in nourishing ingredient and short in moisturizing effect lasting time is solved.
Owner:丁玉琴
Application of artemisinin in preparation of drugs to treat c-myc/mTOR-activated human hepatoma SMMC7721 cells
InactiveCN109908136AProliferation inhibitoryConvenient timeOrganic active ingredientsAntineoplastic agentsMedicineApoptosis
The invention discloses application of artemisinin in the preparation of drugs to treat c-myc / mTOR-activated human hepatoma SMMC7721 cells. Artemisinin is a colorless needle crystal of sesquiterpene lactone with peroxyl groups, extracted from stems and leaves of the compound inflorescence plant Artemisia annua. In the invention, it is discovered for the first time that artemisinin can inhibit theproliferation of human hepatoma SMMC7721 cells, with good time and concentration dependence presented, can induce apoptosis of the human hepatoma SMMC7721 cells by inhibiting the expression of P-c-mycand P-S6 of c-myc / mTOR pathway, and therefore, can be studied and developed as a candidate drug to induce the apoptosis of human hepatoma SMMC7721 cells.
Owner:SOUTHERN MEDICAL UNIVERSITY
Method for characterizing reversible association of macromolecules at high concentration
ActiveUS8195405B2Investigating moving sheetsScattering properties measurementsHigh concentrationLight scattering
A new method is presented for characterizing the associative properties of a solution of macromolecules at high concentration. Sample aliquots spanning a range of concentrations are injected sequentially into a light scattering photometer. Equilibrium association constants and association stoichiometry are derived from an analysis of the angular and concentration dependence of the scattering signals. Thermodynamic nonideality, which becomes important at high concentrations, is dealt with in the analysis in a simplified manner which is applicable to multiple associated species.
Owner:WYATT TECH
Application of 3,5-di-tert-butyl copper salicylate in the preparation of drugs for the treatment of highly metastatic breast cancer
ActiveCN105412125BWide variety of sourcesHigh puritySalicyclic acid active ingredientsAntineoplastic agentsGynecologyAcinic cell carcinoma
The invention discloses the application of 3,5-di-tert-butyl copper salicylate in the preparation of medicines for treating highly metastatic breast cancer and the dosage of 3,5-di-tert-butyl copper salicylate. Experiments have proved that copper 3,5-di-tert-butyl salicylate can inhibit the metastasis of breast cancer cells, and inhibit the invasion of breast cancer cells in a concentration-dependent manner. The invention discovers a new medical application for 3,5-di-tert-butyl copper salicylate, and opens up a new application field.
Owner:JIANGHAN UNIVERSITY
Nrf2/ARE low-oxygen expression and measuring method of protective effects of Nrf2/ARE and astragalus polysaccharides
PendingCN109797129APlay an anti-oxidative stress rolePrevention and treatment of pulmonary heart diseaseMicrobiological testing/measurementVertebrate cellsAstragalus polysaccharideAntioxidative stress
The invention belongs to the technical field of Nrf2 / ARE signal channel regulating effect detection, and discloses Nrf2 / ARE low-oxygen expression and a measuring method of protective effects of Nrf2 / ARE and astragalus polysaccharides. The method includes the steps: culturing HPAECs by an adherence method, building a hypoxia model of the HPAECs, and measuring concentration of Nrf2, SOD and GSH in liquid supernatant of the HPAECs between different groups; detecting expression of endothelial cells Nrf2mRNA, SODmRNA and GSH-PXmRNA by an RT-PCR; measuring protein contents of Nrf2, SOD and GSH-PX byan immunoblotting method. Expression of the HPAECs of a body antioxidant stress signaling channel Nrf2 / ARE in low oxygen is measured, high expression of Nrf2 protein in pulmonary artery endothelial cells are calculated, the astragalus polysaccharides can play antioxidant stress roles by enhancing the high expression of the Nrf2 protein, and the effects of the astragalus polysaccharides have timeand concentration dependence.
Owner:THE FIRST HOSPITAL OF LANZHOU UNIV
Antitumor drug and preparation method and application thereof
InactiveCN109574994AIncrease weightInhibit growthOrganic chemistryAntineoplastic agentsChemical compoundHigh doses
The invention provides an antitumor drug and a preparation method and application thereof. The antitumor drug has the structure as shown as the chemical compound D in the formula I, can effectively inhibit growth of tumor cells and increase weight of mice. Besides, effect of the drug in high dose is better than that of the drug in middle dose, and the effect of the drug in middle dose is better than that of the drug in low dose, in other words, the antitumor drug has concentration dependence. Further, the preparation method of the antitumor drug is simple and easy to operate and can effectively reduce production cost to realize industrialized production of the antitumor drug.
Owner:熊磊
Application of polyketide compound pestalpolyol A as a drug for the treatment of Alzheimer's disease
InactiveCN107823194BConcentration dependentGood for paralysisNervous disorderHydroxy compound active ingredientsDiseasePositive control
The invention discloses an application of polyketone compounds pestalpolyol A as a drug for treating the alzheimer disease. A caenorhabditis elegans model CL4176 of the alzheimer disease is taken as aresearch target, a commercial medicine galanthamine of the alzheimer disease is taken as positive control, and small molecule compounds capable of effectively inhibiting caenorhabditis elegans CL4176paralysis can be effectively screened from a large quantity of natural products or artificially synthesized compounds. A paralysis experiment result proves that compared with the positive control galanthamine, the added polyketone compounds pestalpolyol A with the same concentration have a better effect on inhibiting caenorhabditis elegans paralysis, the inhibition function has concentration dependence in the concentration range of 100 mu M, and the cytotoxicity is smaller.
Owner:YUNNAN UNIV
Determination of protein aggregation from the concentration dependence of delta g
InactiveUS20190120850A1Minimizing aggregationReduce tendency to aggregateBiological testingCell AggregationsProtein aggregation
The present invention relates to, among other things, methods and systems for recognizing and characterizing protein aggregation processes at the earliest possible time and use of such new methods and systems for (1) the identification and selection of protein formulations that minimize aggregation and extend long-term stability and (2) the identification of protein variants with the lowest tendency to aggregate.
Owner:UNCHAINED LABS
Method for characterizing reversible association of macromolecules at high concentration
ActiveUS20100332146A1Easy to analyzeInvestigating moving sheetsScattering properties measurementsHigh concentrationLight scattering
A new method is presented for characterizing the associative properties of a solution of macromolecules at high concentration. Sample aliquots spanning a range of concentrations are injected sequentially into a light scattering photometer. Equilibrium association constants and association stoichiometry are derived from an analysis of the angular and concentration dependence of the scattering signals. Thermodynamic nonideality, which becomes important at high concentrations, is dealt with in the analysis in a simplified manner which is applicable to multiple associated species.
Owner:WYATT TECH
Hairpin-like DNA Templates of Fluorescent Copper Nanoclusters and Their Applications
The invention belongs to the technical field of functional material and fluorescent nano probes, and relates to a novel hairpin DNA template used for synthesis of fluorescent nano copper clusters, and applications of the novel hairpin DNA template in biomolecule detection. The novel hairpin DNA template possesses a hairpin structure; a loop part is composed of poly T with different length, and a stem part is composed of double-stranded DNA with different length and base sequences; and in a certain buffer solution, fluorescence signals can be generated via a few minutes of reaction at room temperature under the action of copper sulfate and vitamin C. Compares with linear poly T, the novel hairpin DNA template possesses following advantages: the fluorescence intensity of the formed fluorescent nano copper clusters is higher, and possesses poly T length and sequence concentration dependence; formation of the fluorescent nano copper clusters is influenced by the stability of the novel hairpin DNA template; formation conditions are mild; reaction is quick; fluorescence intensity is higher than that of the prior art; and biomolecule detection technology without labeling or separating can be designed easily.
Owner:FUDAN UNIV
A lanthanide MOF spun fiber membrane as a sensor for detecting uric acid and its preparation
ActiveCN109283164BHigh selectivityIncreased sensitivityMonocomponent synthetic polymer artificial filamentFluorescence/phosphorescenceFiberMetal-organic framework
Owner:WUHAN DGENSEE CO LTD
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