Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Nrf2/ARE low-oxygen expression and measuring method of protective effects of Nrf2/ARE and astragalus polysaccharides

A technology of astragalus polysaccharide and determination method, which is applied in the directions of biochemical equipment and methods, determination/inspection of microorganisms, biological tests, etc.

Pending Publication Date: 2019-05-24
THE FIRST HOSPITAL OF LANZHOU UNIV
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it is believed that the activation of Nrf2 is a key step for cells to resist oxidative stress, but no research has shown that it is involved in the oxidative stress response of COPD. In the pathophysiological changes of COPD with pulmonary hypertension and cor pulmonale, the hypoxic injury of HPAECs plays an important role. function, and through the regulation of the Keap1 / Nrf2 / ARE pathway, it may become an important method for the prevention and treatment of COPD complications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nrf2/ARE low-oxygen expression and measuring method of protective effects of Nrf2/ARE and astragalus polysaccharides
  • Nrf2/ARE low-oxygen expression and measuring method of protective effects of Nrf2/ARE and astragalus polysaccharides
  • Nrf2/ARE low-oxygen expression and measuring method of protective effects of Nrf2/ARE and astragalus polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0082] In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0083]The present invention aims at the expression of the body's own anti-oxidative stress signaling pathway Nrf2 / ARE in pulmonary artery endothelial cells (HPAECs) under hypoxia, and analyzes the possible mechanism of Nrf2 / ARE signaling pathway in COPD oxidative stress response and the effect of different concentrations of astragalus polysaccharide on Its regulatory role; the adherence method was used to culture HPAECs, and the Nrf2 / ARE signaling pathway was expressed in HPAECs under hypoxia, so as to clarify the mechanism of action of the own antioxidant signaling pathway in the oxidative stress of COPD patients, in order ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of Nrf2 / ARE signal channel regulating effect detection, and discloses Nrf2 / ARE low-oxygen expression and a measuring method of protective effects of Nrf2 / ARE and astragalus polysaccharides. The method includes the steps: culturing HPAECs by an adherence method, building a hypoxia model of the HPAECs, and measuring concentration of Nrf2, SOD and GSH in liquid supernatant of the HPAECs between different groups; detecting expression of endothelial cells Nrf2mRNA, SODmRNA and GSH-PXmRNA by an RT-PCR; measuring protein contents of Nrf2, SOD and GSH-PX byan immunoblotting method. Expression of the HPAECs of a body antioxidant stress signaling channel Nrf2 / ARE in low oxygen is measured, high expression of Nrf2 protein in pulmonary artery endothelial cells are calculated, the astragalus polysaccharides can play antioxidant stress roles by enhancing the high expression of the Nrf2 protein, and the effects of the astragalus polysaccharides have timeand concentration dependence.

Description

technical field [0001] The invention belongs to the technical field of Nrf2 / ARE signaling pathway regulation and detection technology, and in particular relates to a method for measuring the expression of Nrf2 / ARE in hypoxia and its protective effect with astragalus polysaccharide. Background technique [0002] At present, the commonly used existing technologies in the industry are as follows: COPD is a common and frequently-occurring disease that seriously endangers human health. According to the survey conducted by people, the prevalence of COPD in people over 40 years old is as high as 8.2%. According to the data of World Bank and World Health Organization, by 2020, COPD will rank the 5th in the economic burden of disease in the world. The core molecules of the Keapl / Nrf2-ARE signal transduction pathway include NF-E2-related factor-1 (NF-E2-related factor 2, Nrf2), Kelch-like ECH-related protein 1 (Keap1) and antioxidant response element (antioxidant response element , A...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/071C12Q1/02C12Q1/686G01N33/68G01N33/573
Inventor 张甲翠俞登虎刘静李守玺张新栋焦克平
Owner THE FIRST HOSPITAL OF LANZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com