85 results about "Concentration dependent" patented technology

A protein sensing molecule is capable of binding an analyte in a sample. The protein sensing molecule includes a first detectable quality that changes in a concentration dependent manner when the protein sensing molecule is bound to the analyte. The protein sensing molecule also includes a second detectable quality that does not undergo substantial change when the protein sensing molecule is bound to the analyte. The protein sensing molecule may be used in methods for characterizing samples and may also be used in sensors.

The biosensor comprises a modular biorecognition element and a modular flexible arm element. The biorecognition element and the flexible arm element are each labeled with a signaling element. The flexible arm contains an analog of an analyte of interest that binds with the biorecognition element, bringing the two signaling elements in close proximity, which establishes a baseline fluorescence resonance energy transfer (FRET). When an analyte of interest is provided to the biosensor, the analyte will displace the analyte analog, and with it, the signaling module of the modular flexible arm, causing a measurable change in the FRET signal in a analyte concentration dependent manner. The modularity of different portions of the biosensor allows functional flexibility. The biosensor-operates without additional development reagents, requiring only the presence of analyte or target for function.

A headspace instrument (10, 70) operates to equilibrate a vapor phase of a headspace sample in a sample loop (40, 74). The sample is equilibrated by flow through the headspace between variable volume chambers (28, 50, 86, 90). The volume of the variable volume chambers are changed in coordinated relation so that a constant total volume is always maintained. An aliquot of the equilibrated sample in the sample loop is delivered to an analytical instrument. The headspace instrument enables delivering a vapor sample from a headspace under conditions which do not disturb the thermodynamic equilibrium between the vapor phase and the non-vapor phase of the sample and avoids having the sampling process impact the results of the analysis. Alternative forms of the headspace instrument enable the conduct of concentration dependent processes and absorption and desorption experiments.

A method of treating a plastic surface with fluorine gas to decrease adsorption of hydrophobic solute molecules to the surface is provided. The method can include treating a surface with a first gas comprising fluorine gas and a second gas comprising oxygen gas, water vapor, or both oxygen gas and water vapor. Plastics treated using the method provide useful drug discovery and biochemical tools for the testing, handling, and storage of solutions containing low concentrations of hydrophobic solutes. Microfluidic devices containing treated plastic interior surfaces and methods of using such devices to make concentration-dependent measurements are also described.

Disclosed is a library consisting essentially of a plurality of antigen-binding molecules differing in sequence from each other, wherein an antigen-binding domain in each of the antigen-binding molecules comprises at least one amino acid residue that changes the antigen-binding activity of the antigen-binding molecule depending on ion concentration conditions. Also disclosed are a composition comprising a plurality of polynucleotide molecules each encoding the antigen-binding molecules, a composition comprising a plurality of vectors each comprising the polynucleotide molecules, a method for selecting the antigen-binding molecules, a method for isolating the polynucleotide molecules, a method for producing the antigen-binding molecules, and a pharmaceutical composition comprising any of the antigen-binding molecules.

The present invention relates to compositions comprising semi-crystalline beta-1-4-N-acetylglucosamine polymers (p-GlcNac) and methods utilizing such polymers modulation of vascular structure and/or function. The compositions and methods disclosed are useful for stimulating, in a p-GlcNac concentration-dependent manner, endothelin-1 release, vasoconstriction, and/or reduction in blood flow out of a breached vessel, as well as for contributing to or effecting cessation of bleeding. The methods of the present invention comprise topical administration of materials comprising semi-crystalline p-GlcNac polymers that are free of proteins, and substantially free of single amino acids as well as other organic and inorganic contaminants, and whose constituent monosaccharide sugars are attached in a beta-1-4 conformation.

Hydroxylated nebivolol metabolites increase NO release in human endothelial cell preparations in a concentration-dependent manner after acute administration. In addition, hydroxylated nebivolol metabolites (including but not limited to 4-hydroxy-6,6'difluoro) have the ability to increase NO release in human endothelial cells after chronic administration. The present invention provides hydroxylated nebivolol metabolites for use in the treatment of cardiovascular diseases. Furthermore, the present invention provides methods of treating and/or preventing vascular diseases by administering at least one hydroxylated metabolite of nebivolol capable of releasing a therapeutically effective amount of nebivolol to targets affected by vascular diseases. Nitric oxide.

Ulcer is a serious oxidative stress induced disease with complex pathologic events including upregulation of H⁺K⁺ ATPase of parietal cell (PC) membrane, damage of mucin layer around PC, PC-DNA damage etc. The polysaccharide (GRPP) fraction and antioxidant extract (GRAOX) of ginger was examined for their ability to inhibit H⁺ K⁺ ATPase. Results indicated that the inhibition of H⁺ K⁺ ATPase activity which causes acidity in the lumen of the stomach, also exhibited better H⁺K⁺ ATPase inhibition (PPI) at the IC50 of 27.2 μg (GRPP) and 16.5 μg GAE (AOX) respectively than the known antiulcer proton pump blocker Lansoprazole (19.3 μg). Further the antioxidant activity in antioxidant extract (GRAOX) by various assay systems was examined such as Reducing power, Free radical scavenging (FRS), DNA and cytoprotection systems etc. GRAOX exhibited concentration dependent reducing power ability at 5-25 g equivalent of phenol. FRS activity with IC₅₀ of 6.8 μg equivalent of phenol etc. At 0.3 μg level GRAOX offered >80% protection to DNA and against FeSO₄-Asc'orbate induced oxidation. The major active phenolic components were identified as Gallic acid/Tannic acid (46%), and Cinnamic acid (51%).

An objective of the present invention is to provide antibodies that recognize HLA class I and have significant cell death-inducing activity. To achieve the above objective, first, the present inventors immunized mice with soluble HLA-A to which a FLAG tag is attached. Four days after the final immunization, spleen cells from the mice were fused with mouse myeloma cells. Then, hybridoma screening was carried out using the cell aggregation-inducing activity and cell death-inducing activity as an index. Thus, the monoclonal antibody F17B1 that has strong cell death-inducing activity was selected. The sequences of the H chain and L chain variable regions of the mouse monoclonal antibody F17B1 were determined to humanize this antibody. Then, the humanized anti-HLA class I antibody H2-G1d_L1-k0 was assessed for cell death induction. The result showed that H2-G1d_L1-k0 suppressed the growth of the IM9 cell line in a concentration dependent manner.

This invention provides phyllosilicates and polyfunctional dispersants which can be manipulated to selectively control the viscosity of phyllosilicate slurries. The polyfunctional dispersants used in the present invention, which include at least three functional groups, increase the dispersion and exfoliation of phyllosilicates in polymers and, when used in conjunction with phyllosilicate slurries, significantly reduce the viscosity of slurries having high concentrations of phyllosilicates. The functional groups of the polyfunctional dispersants are capable of associating with multivalent metal cations and low molecular weight organic polymers, which can be manipulated to substantially increase or decrease the viscosity of the slurry in a concentration dependent manner. The polyfunctional dispersants of the present invention can also impart desirable properties on the phyllosilicate dispersions including corrosion inhibition and enhanced exfoliation of the phyllosilicate platelets.

The invention discloses a small peptide capable of resisting thrombosis and platelet aggregation, and the sequence of the small peptide is X-Arg-Y-X-Lys, wherein X refers to nonpolar amino acid and the nonpolar amino acid includes alanine, valine, leucine, isoleucine, proline or phenylalanine; while Y refers to polar amino acid and the polar amino acid is glycine, serine, threonine, cysteine, tyrosine, asparagine, glutamine, aspartic acid or glutamic acid. An in vitro platelet aggregation test and an in vivo thrombosis test show that the small peptide can restrain platelet aggregation in a concentration dependent way and simultaneously can remarkably restrain thrombosis of artery-vein bypass in bodies of rats. The small peptide and derivatives of the small peptide can be used for resisting platelet aggregation and thrombosis in treatment. The small peptide can be used for preparing medicaments resisting thrombosis and platelet aggregation.

The invention discloses a process for simultaneously preparing an antioxidative peptide and an antifreeze peptide through enzymolysis of collagens. The method comprises the following steps: extractingcollagens by taking salmon skin as a raw material, hydrolyzing the collagens into a short peptide solution by utilizing crude enzymes of extracellaluar protease obtained by fermentation of Vibrio sp.SQS2-3, performing centrifugal ultrafiltration by a 3000 Da ultrafiltration tube 4500*g for 45 minutes so as to obtain the antifreeze peptide with effects of protecting myosin and relieving decreaseof Ca<2+>-ATPase activity in multigelation, performing Sephadex LH-20 gel filtration chromatography to separate lower ultrafiltration components so as to obtain the antioxidative peptide which has effects of scavenging DPPH free radicals and hydroxyl radicals and a certain ability of inhibiting DNA oxidative damage and shows concentration-dependent antioxidant activity in oxygen radical absorptioncapacity detection. The LC-MS technology shows that the antioxidant component contains 19 polypeptides.

A disclosed preparation method of transgenetic zebrafish used for detecting environment pollutants comprises: (1) cloning the promoter regulatory sequence of Mdr1 protein from zebrafish genome; (2) fusing the DNA fragment obtained by cloning into the upstream of GFP coding sequence for constructing an expression vector; (3) performing zebrafish embryo microinjection on the expression vector; (4) culturing the embryo subjected to injection to sexual maturity, and enabling cultured zebrafish and wide zebrafish to copulate and oviposit; and (5) screening positive F1 generation under a fluorescence microscope, and performing inbreeding to obtain F2 generation and further to obtain a transgenic line with stable inheritance. Because the expression level of fluorescin can be induced in a concentration dependent way by multiple environment pollutants such as heavy metals, polycyclic aromatic hydrocarbons and the like, the transgenic zebrafish prepared by employing the preparation method can be used to detecting the existence and the concentration of the above chemical pollutants in environment; and the preparation method has the advantages of being economic, rapid, convenient and accurate.

The invention discloses a use of psoralen chalcone and its analogues. The use refers to the use of psoralen chalcone or/and its analogues as active ingredients for the preparation of activating retinoic acid-related orphan nuclear receptors. Body-alpha composition. The research results of the present invention show that psoralen chalcone and its analogs can directly activate the activity of RORα in a dose-dependent manner, and activate the expression of RORα protein and RORα mRNA in a concentration-dependent manner, and can effectively regulate its downstream biological clock genes The expression of BMAL1 indicates that psoralen chalcone and its analogues are good positive activators of RORα, which can be used to prevent or treat related diseases mediated by RORα, and have broad application prospects.

The invention belongs to the technical field of preparing compounds, and relates to a compound separated and extracted from marine penicillium and application thereof. The compound is used for preventing and treating in-vitra and in-vivo pseudomonas aeruginosa infection of people or animals, and the chemical formula of the compound which is obtained by fermenting the marine penicillium and has the effect of inhibiting quorum sensing of pseudomonas aeruginosa is 4,6-dimethyl-7-[(1R,2E,4aS,7R,8R,8aR)-1,2.4a,5,6,7,8,8a-octahydro-7-hydroxyl-2-(3-hydroxyl-2-oxygen-propylene)-3,6,8-trimethyl-1-naphthyl]-,(2E,4E,6E)-heptantriene acid, and the molecular formula of the compound is C25H34O5; the compound does not inhibit the growth of the wild type pseudomonas aeruginosa; the quorum-sensing inhibition effect of the compound is concentration-dependent, and is gradually strengthened with the increase of the concentration; virulence factors controlled by the quorum sensing of the pseudomonas aeruginosa can be inhibited; and the compound also has the advantages of readily available raw materials, simple and easily controlled preparation process, obvious sensing inhibition effect and prominent social benefits.

The invention relates to peach blossom extract and an application thereof and particularly relates to an application of peach blossom extract in inhibiting hyaluronidase activity. The peach blossom extract is a solid product which is light yellow or rose red to dark red and is prepared through carrying out ultrasonic extraction on peach blossom through water or a hydrophilic solvent and an aqueoussolution and carrying out reduced pressure concentration distillation drying. The peach blossom extract can be processed into various preparations. In order to verify the effect of the peach blossomextract on the activity of hyaluronidase, the hyaluronidase and peach blossom extract form a reaction system, the absorption value at 400nm of the reaction system is determined, and according to the absorption values of the sample and control, the inhibition ratio of the peach blossom extract to the hyaluronidase is calculated. The result shows that the peach blossom extract has the significant inhibitory effect on the activity of hyaluronidase and the inhibitory effect is increased along with increasing of the application dose. The peach blossom extract has a good concentration-dependent effect and has a semi-suppressed dose (IC50) to the hyaluronidase activity of 0.25 mg/mL.

Labeled annexin is formed by reacting annexin or a modified annexin with a phosphatidylserine group or an analogue of phosphatidylserine. This phosphatidylserine annexin conjugate is then reacted with an appropriate label to form a labeled annexin and the annexin is separated from the phosphatidylserine group. The reaction between the phosphatidylserine group and the annexin is calcium ion concentration dependent. Therefore, the reaction can be promoted by having a high calcium ion concentration and the separation of the phosphatidyl group from the annexin group is facilitated by reducing the calcium ion concentration preferably by addition of an appropriate calcium chelating agent.