Process for preparing antioxidative peptide and antifreeze peptide through enzymatic hydrolysis of salmon collagens

A technology of antioxidant peptides and collagen, applied in the biological field, can solve problems such as safety concerns of livestock collagen products, and achieve the effects of low cost, protective activity, and simple method

Inactive Publication Date: 2018-06-05
CENT SOUTH UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

Traditionally commercially used collagen mostly comes from animal skin and bones, but due to diseases such as

Method used

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  • Process for preparing antioxidative peptide and antifreeze peptide through enzymatic hydrolysis of salmon collagens
  • Process for preparing antioxidative peptide and antifreeze peptide through enzymatic hydrolysis of salmon collagens
  • Process for preparing antioxidative peptide and antifreeze peptide through enzymatic hydrolysis of salmon collagens

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Embodiment 1

[0033] a use Vibrio sp. SQS2-3 extracellular protease enzymatically hydrolyzes salmon skin collagen to prepare antifreeze peptides and antioxidant peptides, the steps are as follows:

[0034]1. Bacteria Vibrio The sp. SQS2-3 strain was inoculated in the liquid seed medium, cultured in a shaker at 17°C for 2-3 days with a rotation speed of 200 rpm, and then inoculated into the fermentation medium at an inoculum volume of 2% (v / v) In 17 ° C horizontal shaker for 5 days, the rotation speed is 180 rpm, and the fermentation broth is obtained;

[0035] The above-mentioned seed medium components are as follows, all in parts by weight: 0.5 part of peptone, 0.1 part of yeast powder, Fe 2 (PO 4 ) 3 0.001 parts, artificial seawater 100 parts, pH 7.8;

[0036] The components of the above-mentioned fermentation medium are as follows, all in parts by weight: 1 part of corn flour, 0.5 part of bran, 1 part of soybean meal, Na 2 HPO 4 0.05 parts, KH 2 PO 4 0.02 parts, CaCl 2 0....

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Abstract

The invention discloses a process for simultaneously preparing an antioxidative peptide and an antifreeze peptide through enzymolysis of collagens. The method comprises the following steps: extractingcollagens by taking salmon skin as a raw material, hydrolyzing the collagens into a short peptide solution by utilizing crude enzymes of extracellaluar protease obtained by fermentation of Vibrio sp.SQS2-3, performing centrifugal ultrafiltration by a 3000 Da ultrafiltration tube 4500*g for 45 minutes so as to obtain the antifreeze peptide with effects of protecting myosin and relieving decreaseof Ca<2+>-ATPase activity in multigelation, performing Sephadex LH-20 gel filtration chromatography to separate lower ultrafiltration components so as to obtain the antioxidative peptide which has effects of scavenging DPPH free radicals and hydroxyl radicals and a certain ability of inhibiting DNA oxidative damage and shows concentration-dependent antioxidant activity in oxygen radical absorptioncapacity detection. The LC-MS technology shows that the antioxidant component contains 19 polypeptides.

Description

technical field [0001] The invention relates to a method for simultaneously preparing antioxidant peptides and antifreeze peptides by using microbial protease to enzymolyze salmon skin collagen, and belongs to the technical field of biotechnology. Background technique [0002] my country has a vast area of ​​ocean and fresh water, rich in aquatic resources, accounting for 36% of the world's total aquatic products. With the development of fishery, the development of aquatic product processing industry has also been promoted. According to statistics, about 20 million tons of protein-rich fish by-products, such as fish bones, fish skin, fish fins and fish scales, are produced during processing every year, and most of them are directly buried and discarded or used to make animal feed , Fishmeal and other low-value products not only cause a serious waste of resources, but also directly lead to environmental pollution. Therefore, it has become an important means to alleviate res...

Claims

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Application Information

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IPC IPC(8): C12P21/06C12N9/52C07K14/78C07K1/34C07K1/16
CPCC07K14/78C12N9/52C12P21/06
Inventor 何海伦吴日帮杨兴昊刘丹武翠玲张姜黄嘉丰廖斌强
Owner CENT SOUTH UNIV
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