30 results about "DNA oxidation" patented technology

The methods of the invention can be used to protect against lymphocyte DNA damage and free-radical associated disorders in a subject. The methods of the present invention can be used to increase the antioxidant capacity in both the aqueous and lipid compartments, decrease DNA oxidation, decrease lipid peroxidation, and increase antioxidant nutrient levels in the circulation. The protective effect of the physiologic dose of the mixed carotenoid supplement is rapid, consistent and cumulative.

The invention relates to an assessment method on the protective action of Chinese medicinal herb to the DNA oxidative damage, which comprises the steps that: DNA is fixed on the surface of a magnetic bead microsphere by covalent bonds; Chinese medicinal herb solution is added; the fixed DNA infects toxicity in vitro; magnetic bead separation is carried out to oxidize the components but reserve the oxidation-damaged DNA; 8-OH-dG antibodies are added to carry out immunoreaction with the 8-OH-dG generated by oxidative damage; enzyme-labeled second antibodies are added to react with the obtained product; and a luminous substrate is added, the high-sensitivity chemiluminescence method is adopted to detect the generated 8-OH-dG by DNA damage; the detection result is analyzed according to the detection result of the control group so as to judge whether the Chinese medicinal herb has the function of inhibiting the DNA oxidative damage to generate 8-OH-dG. The invention can detect and assess the protective action of Chinese medicinal herb to the DNA oxidative damage, and can be used for screening and developing high-efficiency and safe Chinese herbal preparations to protect the organism DNA from suffering oxidative damage.

The invention discloses a method for high performance liquid chromatography (HPLC)-mass spectrometry (MS) detection of DNA oxidation and DNA methylation, and the method comprises the following steps: hydrolyzing DNA, measuring the content of 5-methyl cytosine (5-mdC) and 8-hydroxy deoxyguanosine (8-OHdG) in the DNA hydrolysis product by HPLC-ESI (electrospray ionization)-MS / MS; wherein the high performance liquid chromatography is performed as follows: a mobile phase A is formic acid / methanol with the volume ratio of 0.01-0.3, a mobile phase B is a formic acid water solution with the volume ratio of 0.01 to 0.3%, and under the conditions of the flow velocity of 0.2-0.4ml / min and the column temperature of 15 to 40 DEG C, separation is performed by a liquid chromatography column; ESI (electrospray ionization)-MS is performed as follows: an electrospray ion source is ionized, and mass spectrometric analysis is performed in the positive electrode mode by a multiple reaction monitoring system. The method for high performance liquid chromatography (HPLC)-mass spectrometry (MS) detection of DNA oxidation and DNA methylation can simultaneously detect the content of 8-OHdG and 5-mdC in a DNA sample.

The invention discloses a process for simultaneously preparing an antioxidative peptide and an antifreeze peptide through enzymolysis of collagens. The method comprises the following steps: extractingcollagens by taking salmon skin as a raw material, hydrolyzing the collagens into a short peptide solution by utilizing crude enzymes of extracellaluar protease obtained by fermentation of Vibrio sp.SQS2-3, performing centrifugal ultrafiltration by a 3000 Da ultrafiltration tube 4500*g for 45 minutes so as to obtain the antifreeze peptide with effects of protecting myosin and relieving decreaseof Ca<2+>-ATPase activity in multigelation, performing Sephadex LH-20 gel filtration chromatography to separate lower ultrafiltration components so as to obtain the antioxidative peptide which has effects of scavenging DPPH free radicals and hydroxyl radicals and a certain ability of inhibiting DNA oxidative damage and shows concentration-dependent antioxidant activity in oxygen radical absorptioncapacity detection. The LC-MS technology shows that the antioxidant component contains 19 polypeptides.

The invention discloses an experimental method of effects of DNA oxidative damage and bone cell apoptosis on avascular necrosis, the method comprises the following steps: experimental grouping and establishment of an animal model, preparation of specimens, histomorphology observation under a light microscope, observation by a transmission electron microscope, bone cell apoptosis situation detection by TUNEL (terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling) method, bone marrow hematopoietic cell DNA oxidative damage detection by monoclonal antibody N45.1 immunohistochemistry method, and statistical analysis. According to the method, experiment animals are respectively killed in the second week and the fourth week after the last time of administration, and five animals are killed for each time in each group; bilateral femoral heads are taken for conventional fixed decalcification for stand-by testing, the number of vacant bone lacunas is counted by HE (hematoxylin-eosin) staining, the bone cell morphological change is observed by the electron microscope, the bone cell apoptosis situation is detected by the TUNEL method, the bone marrow hematopoietic cell DNA oxidative damage is detected by the immunohistochemistry method, and the relationship between SANFH ((steroid-induced avascular necrosis of femoral head)) and the bone marrow hematopoietic cell DNA oxidative damage and the bone cell apoptosis is explored, and provides new ideas for the further study of pathogenesis of the steroid induced avascular necrosis of femoral head.

The invention belongs to a cell DNA detection technology and in particular relates to a method for detecting 8-hydroxy-2'-deoxyguanosine (8-OHdG) and 2'-deoxyguanosin (dG) in cell DNA. The method can be used for quantitatively detecting the change of the content of 8-OHdG and dG in cell DNA, caused by exposure of harmful ingredients in cigarette smoke, so that the purpose of evaluating DNA oxidation damage caused by induction of the harmful ingredients can be achieved. According to the method, deoxyribonuclease I and alkaline phosphatase are used for hydrolyzing DNA in a cell, and deferoxamine mesylate is added into enzymatic hydrolysate, so that the oxidation of dG in the enzymolysis process is avoided; enzymatic hydrolysate is detected by HPLC-MS/MS; the cell DNA oxidation damage degree is evaluated according to the value of 8-OHdG/10<6>dG. By virtue of the method, the interference of high-concentration metal ions and artificial dG oxidation is removed, and thus the result is relatively accurate; the method is low in detection limit, high in sensitivity, good in repeatability and high in recovery rate.

The invention belongs to the field of pharmacy and relates to a new application of a traditional Chinese medicine motherwort extract in pharmacy, in particular to the application of the purified motherwort extract in preparing nerve protection medicine for treating cerebral injury, especially the application of the purified motherwort extract in preparing brain protection medicine for treating artery obstruction in the brain. By an animal experiment of the purified motherwort extract, a result indicates that the brain stem volume is obviously reduced after the purified motherwort extract is applied, the obstacle grading of the nerve function is decreased by being compared with a contrast group, the total antioxidant concentration in plasma is increased, the DNA oxidation injury is reduced, the expression levels of apoptosis and apoptosis promoting protein are reduced, and the content of apoptosis resistance protein is also increased. The purified motherwort extract can be used for preparing the nerve protection medicine for treating the cerebral injury and can effectively treat cerebral apoplexy.

The invention discloses aesculetin with anti-oxidative and anti-aging activity as well as a preparation method and an application of aesculetin. The preparation method comprises preparation steps as follows: (1), cortex fraxini residues are dried, sterilized for 30 min under ultraviolet irradiation and taken out; (2), distilled water which is 8 times the sterilized cortex fraxini residues in parts by weight is added to the sterilized cortex fraxini residues for fermentation, after fermentation, a sample is taken out, dried, smashed and screened by a 30-mesh sieve, and fermented cortex fraxini residue powder is obtained; (3), an organic extracting agent is added to the fermented cortex fraxini residue powder for reflux extraction, extracting solutions are combined, a combined solution is filtered, and the organic extracting agent is subjected to reduced-pressure recovering and concentrated into thick paste; (4), distilled water is added to the thick paste for dilution, heating dissolution is performed, an obtained solution is filtered while still hot, and a filtrate is separated, purified and decolorized and then is subjected to reduced-pressure concentration and freeze drying finally. Aesculetin has a function of eliminating oxygen free radicals, has a protecting effect on cell DNA oxidative damage caused by lipid peroxide and can effectively delay skin cell senescence.

The invention discloses an oxidative damage DNA detection method, and relates to the technical field of DNA oxidative damage detection. The method comprises (a) obtaining of DNA containing 3 ', 5'-phosphate group nucleotide voi, to be more specific, adding a DNA repair enzyme for recognition and cutting of oxidized DNA bases; (b) introduction of hydroxyl into 3 ', 5' site, to be more specific, adding alkaline phosphatase for dephosphorylation to obtain the DNA containing 3 ', 5'-hydroxyl group nucleotide voids; (c) introduction of a fluorescent marker, to be more specific, adding DNA polymerase I and the fluorescent marker to obtain fluorescence-labeled DNA; (d) formation of a PFP-DNA complex, to be more specific, adding PFP to obtain the PFP-DNA complex; and (e) oxidative damage DNA detection, to be more specific, detecting damage DNA by FRET. The method alleviates the disadvantages of tedious steps, low sensitivity and low selectivity of DNA damage detection in the prior art. The method has the advantages of high sensitivity, strong specificity and high accuracy.

The invention provides a photosensitive dye osmium complex and a preparation method thereof, and a kit and a method for performing quick canceration early stage deoxyribonucleic acid (DNA) oxidative damage detection. The photosensitive dye osmium complex has the structural general formula of LL'L''Os-Y4, wherein Y is a halide ion, ClO<4->, BF<4->, PF<6-> or OTs<->; L and L' are independently selected from 2,2'-dipyridyl and a derivative thereof, phenanthroline and a derivative thereof and DPPZ; and L'' is a precursor with 2,2'-dipyridyl and 4,4'-dipyridyl. The kit at least comprises a main body compound 8-element cucurbit urils (a component A), the photosensitive dye osmium complex (a component B) and pure water or phosphate and borate buffering solution (a component D). By adopting the kit, even under the condition of no instrumental equipment, a user can individually and quickly detect 8-oxoG under visible light with eyes only by one drop of blood.

The invention provides a bee pollen preparation. The bee pollen preparation is obtained by mixing three kinds of broken wall bee pollen together and extracting an active ingredient from bee pollen through ethanol. The active ingredient comprises 17.92+/-1.13mg/g of p-cumaric acid, 69.83+/-0.95mg/g of quercetin, 8.72+/-0.64mg/g of isorhamnetin, 0.96+/-0.07mg/g of naringenin, 24.34+/-0.93mg/g of kaempferol, and 21.75+/-1.02mg/g of galangin. The bee pollen preparation has a high oxidizing ability of buckwheat bee pollen, meanwhile has a high complexing power of oilseed rape bee pollen and broomcorn bee pollen, and can be used for preparing drugs or healthy food for preventing DNA oxidative damage. As certified by animal pharmacodynamics experiments, the bee pollen has the effects of enhancingserum oxidation resistance and preventing DNA oxidative damage, and can be used for preparing drugs or healthy food for preventing DNA oxidative damage.

The invention provides an application of a reagent capable of detecting peripheral blood mitochondria DNA oxidative damage in preparation of a reagent for evaluating in-vivo oxidation stress, and a method for evaluating in-vivo oxidation stress based on detection of peripheral blood mitochondria DNA oxidative damage.

The invention relates to the technical field of bio-medicine engineering and discloses an interfering RNA (Ribonucleic Acid) for inhibiting expression of an echinococcus granulosus protoscolex DNA (Deoxyribonucleic Acid) oxidative damage repairing gene and application of the interfering RNA. The interfering RNA for inhibiting expression of the echinococcus granulosus protoscolex DNA oxidative damage repairing gene has target sequences of forward CCGUGUUCGUAAGCAGCUUTT and backward AAGCUGCUUACGAACACGGTT. The invention discloses the target sequences of the interfering RNA for inhibiting expression of the echinococcus granulosus protoscolex DNA oxidative damage repairing gene for a first time, and also discloses application of the interfering RNA in preparing cystic echinococcosis medicines for the first time. By inhibiting expression of the EgRPS9 gene by using the interfering RNA, growth of echinococcus granulosus protoscolex is inhibited and killed by affecting the repairing function of DNA oxidation damage of the echinococcus granulosus protoscolex, so that cystic echinococcosis can be effectively treated.

The invention discloses a signal labeled molecule for specific detection of a DNA oxidative damage product 8-hydroxydeoxyguanosine and a labeling method. The signal labeled molecule at least contains two functional groups: one functional group can be connected to 8-hydroxydeoxyguanosine through a covalent bond; and the other functional group can generate a detection signal. The signal labeled molecule specifically reacts with 8-hydroxydeoxyguanosine in the presence of a certain concentration of an oxidizing agent so as to form covalent labeling. According to the signal generated by the labeled molecule, a corresponding detection method can be adopted. By measuring a signal such as an optical signal, an electric signal, an electrochemiluminescence signal or a photo-electrochemical signal and the like which is generated by the labeled molecule, quantitative detection of 8-hydroxydeoxyguanosine is detected. The labeled molecule finishes bonding and signal labeling of 8-hydroxydeoxyguanosine through a one-step reaction, and reaction conditions are mild. The signal labeled molecule has good specificity and high labeling rate, and is very suitable for quick and quantitative detection of 8-hydroxydeoxyguanosine.

The present invention discloses a kind of Chinese medicine composition with antioxidant and immunoregulating effects, and the Chinese medicine composition is the concentrated solution of water extracted solution of Chinese medicinal materials clove and raspberry in the same weight. The present invention proves the antioxidant effect of the Chinese medicine composition by means of hydroxy radical eliminating experiment, DNA oxidizing damage inhibiting experiment and rat abdominal cavity macrophage respiration burst suppression experiment, and verifies the antioxidant and immunoregulating effects of the Chinese medicine composition through oxidation stress mouse model experiment. The Chinese medicine composition with antioxidant and immunoregulating effects may be used as medicine for preventing and treating senile dementia.

The invention relates to application of the polypeptide HDP-1 of Haliotis discus hannai to preparation of drugs, health products or dietary supplements used for preventing and treating tumors and diseases related to oxidation. HDP-1 has strong free radical removing capability and can inhibit free radical-mediated DNA oxidation damage. HDP-1 can also inhibit the migration of HT1080 cells and the expression of MMP-2 and MMP-9, and the inhibitory intensity of HDP-1 is dose-dependent; that is, HDP-1 has antioxidant and antitumor activities.

The invention discloses a Chinese medicament preparation for protecting DNA oxidative damages, a preparation method and application thereof. The Chinese medicament preparation is prepared from the following raw materials: rose myrtle seed, ginkgo, glossy ganoderma and fructus cannabis. The preparation method of the Chinese medicament preparation comprises the following steps: soaking the rose myrtle seed, the ginkgo, the glossy ganoderma and the fructus cannabis in water respectively; decocting the glossy ganoderma first, and then adding the other raw materials for decoction; filtering the decoction, and concentrating the filtrate; and filtering the decoction again to obtain a Chinese medicament crude extract for standby. The Chinese medicament preparation is favorable for improving the anti-DAN oxidation defense function of an organism in an oxidative stress state, has inoxidability, and can be prepared into anti-ageing medicaments and anti-cancer health products. The crude extract ofthe Chinese medicament preparation also serves as an active component which is combined with other acceptable adjuvant or carriers to form a novel anti-ageing health product with the anti-DAN oxidation function.

The invention relates to application of a shippocampus extract 2H5M in preparation of a drug, a health product or a dietary supplement for preventing and treating neurodegenerative diseases. The chemical name of the shippocampus extract 2H5M is 2'-hydroxyl-5'-methoxyacetophenone. The shippocampus extract 2H5M is from hippocampus kuda bleeler, does not have cytotoxicity to mouse microglia, can inhibit generation of NO and ROS in the mouse microglia, and can lower DNA oxidative damage.

Disclosed are a DNA oxidative damage repairing device and an application method thereof. The device is composed of an electrons emitting assembly, an electrons emitting electrode, a control system, a power source, a support and the like. The device emits electrons to a space in a certain range, negatively charged nano particles with certain density are formed in the space, the space is sterilized, and various hazardous substances in suspension space are eliminated. The negatively charged nano particles entering a body through a respiratory system release electrons carried by the particles, electrons lost due to oxidative damage are replenished to all parts of the body, and dormant state genes can also be repaired and activated. The two-way regulations function can play a role in broad spectrum medical treatment, so that diseases that cannot be cured through drugs easily are present are cured. The device has an obvious curative effect on diseases caused by immune function deficiencies, diseases of a respiratory system, various wounds, diseases caused by drug addiction treatment and the like. The method is combined with correct and effective using of suppressing drugs and accurate using of traditional Chinese medicine, and accordingly a novel hopeful therapeutic scheme of treating AIDS, cancers and various genetic diseases before histone codes are deciphered can be provided.