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Biological method for detecting genetic toxicity of polluted water body

A genotoxicity and biological technology, applied in the field of quantitative evaluation of genotoxicity effects in polluted water, can solve the problems of complex genotoxicity monitoring methods in water bodies, and achieve the effects of short experimental period, good reproducibility and simple operation

Inactive Publication Date: 2015-09-23
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, our country has not yet been included in the routine monitoring of water sources. The main reason is that the monitoring methods of genotoxicity in water are relatively complicated

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Evaluation of the genotoxic effects of drainage from the secondary sedimentation tank of a certain urban sewage treatment plant

[0036] The first step, domestication test: select healthy carp with a body length between 4-5cm, and domesticate them for 7 days in tap water that has been aerated for 24 hours to dechlorinate and treated with activated carbon adsorption. 5%, if it is higher than 5%, a new batch of carp will be domesticated again.

[0037] The second step, exposure test: put 10 carp in 3 glass beakers with 10L treated tap water samples and 10L treated tap water samples respectively for exposure test, exposure time for 10 days.

[0038] The third step, 8-OHdG detection: take the liver of carp and place it in a beaker, and then fully homogenize it with a homogenizer. The homogenate was transferred to a centrifuge tube, centrifuged at 5000rpm for 10 minutes, and the supernatant was taken. The 8-OHdG concentration in the supernatant was detected by a...

Embodiment 2

[0041] Example 2: Evaluation of the genotoxic effect of wastewater from a pharmaceutical factory

[0042] The first step, domestication test: select healthy zebrafish with a body length of 2-3cm, put them into artificially prepared dilution water and domesticate them for 12 days, and ensure that the mortality rate of zebrafish is lower than 5% during the domestication period. Then change a batch of zebrafish to re-domesticate.

[0043] The second step, exposure test: 15 zebrafish were placed in 3 glass beakers containing drainage water samples from a certain pharmaceutical factory and 1 glass beaker containing 8L of artificially prepared dilution water for the exposure test, and the exposure time was 7 days.

[0044] The third step, 8-OHdG detection: take the whole fish of zebrafish and place it in a beaker, and then fully homogenize it with a homogenizer. The homogenate was transferred to a centrifuge tube, centrifuged at 5000rpm for 10 minutes, and the supernatant was taken...

Embodiment 3

[0047] Example 3: Evaluation of the genotoxic effects of surface water in a certain river

[0048] The first step, domestication test: choose healthy medaka fish with a body length of 2-3cm, put 15 medaka fish in glass beakers with artificially prepared dilution water for exposure test, and the exposure time is 10 days.

[0049] The second step, exposure test: 15 medaka fishes were placed in 3 glass beakers with 10L of surface water samples of a certain river and 1 glass beaker with 10L of artificially prepared dilution water, and the exposure time was 10 days. .

[0050] The third step, 8-OHdG detection: take the whole fish or liver of medaka fish and place it in a beaker, and then fully homogenize it with a homogenizer. The homogenate was transferred to a centrifuge tube, centrifuged at 1000rpm for 5 minutes, and the supernatant was taken. The 8-OHdG concentration in the supernatant was detected by an enzyme-linked immunosorbent immunoassay (ELISA) kit, and the 8-OHdG conce...

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Abstract

The invention relates to water quality monitoring methods in the field of environmental protection, in particular to a testing method for quantitatively evaluating the genetic toxicity effect of a polluted water body by using a DNA (deoxyribonucleic acid) oxidative damage product-8-hydroxy deoxyguanosine (8-OHdG) as a marker. The method is based on DNA oxidative damage, is a most common biological mechanism for genetic toxicity of organisms caused by exogenous pollutants, takes 8-OHdG as the marker for detecting DNA oxidative damage degree of the polluted water body to the interior of a fish body, and takes 4-nitroquinoline-1-oxide (4NQO) or benzo[a]pyrene as a positive reference for quantitatively evaluating the genetic toxicity effect of the polluted water body. The method can comprehensively evaluate the genetic toxicity effect of the polluted water body on the organisms, has the advantages of simplicity for operation, short experimental cycle, sensitivity for response, good repeatability and the like, is very suitable for quantitatively evaluating the genetic toxicity of various polluted water bodies, and has a wide application prospect in the water quality monitoring work.

Description

technical field [0001] The invention relates to a water quality monitoring method in the field of environmental protection, in particular to a test method for quantitatively evaluating the genotoxic effect of polluted water bodies by using 8-hydroxydeoxyguanosine (8-OHdG), a DNA oxidation damage product, as a marker. Background technique [0002] Since the 20th century, with the rapid development of the chemical industry, a large number of known or newly synthesized toxic and harmful chemicals have been continuously discharged into the water environment, causing serious pollution and seriously affecting the health of humans and environmental organisms. Among these many pollutants, quite a few have genotoxic effects on the human body and environmental organisms, and can cause tumors and even cancer diseases in the body. Such pollutants can not only damage the genetic material of organisms and induce cancer, but can even be transmitted vertically to offspring through reproduct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/18G01N30/00G01N33/50G01N33/53
CPCY02A20/20
Inventor 谢显传孙捷黄玉薛银刚李爱民张幼宽
Owner NANJING UNIV
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