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A composition (RCUD) for protecting and/or repairing DNA from oxidative damages and a method thereof

A technology of oxidative damage and composition, applied in the direction of anhydride/acid/halide active ingredients, drug combinations, pharmaceutical formulations, etc., can solve problems such as not mentioned

Inactive Publication Date: 2006-05-10
印度科学工业研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the role of urine or its distillates as a protector of cells from DNA damage and chromosomal variations is not mentioned in the literature and scriptures

Method used

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  • A composition (RCUD) for protecting and/or repairing DNA from oxidative damages and a method thereof
  • A composition (RCUD) for protecting and/or repairing DNA from oxidative damages and a method thereof
  • A composition (RCUD) for protecting and/or repairing DNA from oxidative damages and a method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0047] In the following steps, Kamdhenu Ark was demonstrated to actinomycin D, H 2 o 2 and MnO 2 Protection against DNA damage caused. In this effort, applicants tested actinomycin D, H 2 o 2 and MnO 2 DNA damage against polymorphonuclear leukocytes and protection by modified Kamdhenu Ark (redistilled cow urine distillate) obtained as described above. These experiments are described in the Examples below.

[0048] Protection against DNA damage was observed whether polymorphonuclear leukocytes were pretreated with redistilled cow urine distillate or polymorphonuclear leukocytes were treated with redistilled cow urine distillate when DNA damaging chemicals were added .

[0049] In fact, redistilled cow urine distillate also had a statistically significant reduction in DNA damage caused by the aforementioned DNA damaging agents.

[0050] The embodiment provided below is only a detailed description of the invention of the application, and should not be construed as a

[0...

Embodiment -I

[0053] The protective effect of redistilled cow urine distillate on the DNA damage of polymorphonuclear leukocytes induced by the DNA damaging chemical agent actinomycin-D was elaborated (Table-2).

[0054] Table 2: DNA damage induced by actinomycin D and

[0055] Protective effect of redistilled cow urine distillate (modified K-Ark)

[0056] Experiment Details

Group I

Group II

Group III

Group IV

Group V

average

SD

P value

control

(0.1%DMSO)

70

75

69

66

69

70

2.92

-

Kamdhenu

Ark (improved)

70

70

68

74

72

71

2.28

NS

Actinomycin D

(positive control)

40

38

44

28

39

38

5.30

* P＜0.001

Kamdhenu

Ark (60μl)

+ Actinomycin D

(preprocessing)

43

...

Embodiment -2

[0062] Elaborate on the effect of double-distilled cow urine distillate on the DNA damage chemical reagent hydrogen peroxide (H 2 o 2 ) Protective effect on DNA damage caused by polymorphonuclear leukocytes (Table-3).

[0063] Table 3: Hydrogen peroxide (H 2 o 2 ) DNA damage caused by and

[0064] Protective effect of redistilled cow urine distillate

[0065]

Experiment Details

Group

I

Group

II

Group

III

Group

IV

Group

V

average

value

SD

P value

control

(0.1%DMSO)

80

71

70

71

87

76

2.92

-

Kamdhenu

Ark (unmodified)

70

70

68

74

72

71

2-28

NS

hydrogen peroxide

(H 2 o 2 )

(positive control) ...

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PUM

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Abstract

A composition useful for protecting and / or repairing DNA from oxidative damages said composition comprising redistilled cow's urine distillate (RCUD) having components benzoic acid, and hexanoic acid, with ammonia content of the composition ranging between 5-15mg / L, and optionally along with anti-oxidants; and a method of protecting and / or repairing DNA from oxidative damages using composition of claim 1, said method comprising steps of estimating the amount of folded DNA in a sample, mixing the said composition to the said DNA either before or after the exposure of the DNA to the oxidatively DNA-damaging agent, and determining percentage folded DNA in the mixture showing protection and / or repair of DNA from oxidative damages.

Description

technical field [0001] A composition useful for protecting and / or repairing DNA from oxidative damage, said composition comprising redistilled cow urine distillate (RCUD), wherein RCUD contains benzoic and caproic acid components, the ammonia content of the composition in the range of 5-15 mg / L, and optionally containing antioxidant; and a method of protecting and / or repairing DNA from oxidative damage using the composition of claim 1, said method comprising the steps of: estimating folded DNA in a sample The composition is mixed into the DNA before or after exposure of the DNA to the DNA oxidative damage agent, and the percentage of folded DNA in the mixture is determined, which represents protection against DNA oxidative damage and / or repair. Background technique [0002] In the Vedas, cow products are compared with divine wine (Rig Veda 10.15pp.47). In Ayurveda, cow urine is one of the ingredients of Panchagavya. According to Susrut, cow urine has several medicinal pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/22A61K31/19A61K31/192A61K33/02A61K45/06
CPCA61K31/19A61K31/192A61K33/02A61K35/22A61K45/06A61P39/06A61K2300/00
Inventor 查卡巴提·他潘萨拉瓦纳·德维·斯万赛克里什那莫提·坎南都塔·迪潘威塔辛·里施·那瑞恩曼辛卡·苏尼尔·巴克里施那道尔·苏伊什·哈里鲍
Owner 印度科学工业研究所
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