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Bee pollen preparation and preparation method and application to preventing DNA oxidative damage

A technology of oxidative damage and bee pollen, applied in the application, pharmaceutical formula, function of food ingredients, etc., can solve the problems of DNA oxidative damage prevention function and low content of active ingredients, etc., to prevent DNA oxidative damage and prevent senile dementia. , the effect of enhancing antioxidant capacity

Active Publication Date: 2018-05-29
陕西康泰莱生物医药工程有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The patent "Schisandra bee pollen effective parts and its application in the prevention of liver damage" proposed by Cao Wei et al. limited the extraction method and components of the active parts of Schisandra bee pollen, and did a functional evaluation experiment to protect the liver, but the active ingredients The content is not high, and it is not involved in the prevention of DNA oxidative damage

Method used

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  • Bee pollen preparation and preparation method and application to preventing DNA oxidative damage
  • Bee pollen preparation and preparation method and application to preventing DNA oxidative damage
  • Bee pollen preparation and preparation method and application to preventing DNA oxidative damage

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Take 500g of broken wall rape bee pollen, 300g of broken wall buckwheat bee pollen, and 200g of broken wall sorghum bee pollen, mix them, add 10L of 60% ethanol; extract twice by heat reflux at 60°C, and extract after 2 hours each time liquid, combined the 2 extracts and filtered to obtain the filtrate. The filtrate was concentrated under reduced pressure at 45°C to an extract state.

[0049] The above extract was dissolved in 10 times the weight of methanol solution with a concentration of 70%, extracted 2 times with equal volumes of ethyl acetate, each extraction was 20min, after merging the ethyl acetate extracts obtained by the 2 extractions, and It was concentrated under reduced pressure to an extract.

[0050] Dissolve the extract in hot water at 70°C, adsorb it with XAD-2 resin, rinse with distilled water of 2 times the volume of the column bed, and then elute with 80% methanol solution of 2 times the volume of the column bed, and concentrate the eluent to Past...

Embodiment 2

[0054] Take 500g of broken wall rape bee pollen, 300g of broken wall buckwheat bee pollen, and 200g of broken wall sorghum bee pollen, mix them, add 12L of 70% ethanol; extract 3 times by heat reflux at 70°C, and extract after 2 hours each time liquid, combined three extracts and filtered to obtain the filtrate. The filtrate was concentrated under reduced pressure at 50°C to an extract state.

[0055] Dissolve the above extract in 10 times the weight of methanol solution with a concentration of 80%, extract 3 times with equal volume of ethyl acetate for 30 min each time, combine the ethyl acetate extracts obtained by 3 times of extraction, and extract it Concentrate under reduced pressure to extract.

[0056] Dissolve the extract in hot water at 80°C, adsorb it with XAD-2 resin, rinse with distilled water of 2 times the volume of the column bed, and then elute with 90% methanol solution of 3 times the volume of the column bed, and concentrate the eluent to Paste; the resulti...

Embodiment 3

[0060] Take 500g of broken wall rapeseed bee pollen, 300g of broken wall buckwheat bee pollen, and 200g of broken wall sorghum bee pollen, mix them, add 15L of 80% ethanol; extract 4 times by heat reflux at 80°C, and extract 3 hours each time to get The extracts were combined for 4 times and filtered to obtain a filtrate. The filtrate was concentrated under reduced pressure at 55°C to an extract state.

[0061] Dissolve the above-mentioned extract in 10 times the weight of methanol solution with a concentration of 85%, extract 4 times with equal volume of ethyl acetate, extract 30 min each time, combine the ethyl acetate extracts obtained by 4 times of extraction, and extract it Concentrate under reduced pressure to extract.

[0062] The extract was dissolved in hot water at 85°C, adsorbed with XAD-2 resin, washed with distilled water of 2 times the volume of the column bed, and then eluted with 100% methanol solution of 4 times the volume of the column bed, and the eluate wa...

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Abstract

The invention provides a bee pollen preparation. The bee pollen preparation is obtained by mixing three kinds of broken wall bee pollen together and extracting an active ingredient from bee pollen through ethanol. The active ingredient comprises 17.92+ / -1.13mg / g of p-cumaric acid, 69.83+ / -0.95mg / g of quercetin, 8.72+ / -0.64mg / g of isorhamnetin, 0.96+ / -0.07mg / g of naringenin, 24.34+ / -0.93mg / g of kaempferol, and 21.75+ / -1.02mg / g of galangin. The bee pollen preparation has a high oxidizing ability of buckwheat bee pollen, meanwhile has a high complexing power of oilseed rape bee pollen and broomcorn bee pollen, and can be used for preparing drugs or healthy food for preventing DNA oxidative damage. As certified by animal pharmacodynamics experiments, the bee pollen has the effects of enhancingserum oxidation resistance and preventing DNA oxidative damage, and can be used for preparing drugs or healthy food for preventing DNA oxidative damage.

Description

【Technical field】 [0001] The invention relates to a preparation method of a bee pollen preparation and its application in preventing DNA oxidative damage, and belongs to the technical field of medicine and functional food. 【Background technique】 [0002] Oxidative stress refers to the massive production of active oxygen free radicals in cells, or the weakening of scavenging ability, so that free radicals accumulate in cells, so the body produces oxidative stress. Oxidative stress can lead to damage such as DNA strand breaks and site mutations, accelerate cell aging and apoptosis, and cause many diseases, such as Alzheimer's disease, Parkinson's disease, diabetes, hypertension, stroke, etc., are more likely Cause malignant diseases such as cancer and tumors. [0003] At present, there are many researches on the anti-oxidation of bee pollen, but there are few related product developments. Although Shao Suying's patent "A Bee Pollen for Improving Immunity and Its Preparation ...

Claims

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Application Information

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IPC IPC(8): A61K36/899A23L33/105A61P25/28A61P3/10A61P9/12A61P9/10A61P25/16
CPCA23V2002/00A61K36/31A61K36/70A61K36/899A61K2236/333A61K2236/39A61K2236/51A61K2236/55A23L33/105A61K2300/00A23V2200/322A23V2200/328A23V2200/326A23V2250/21168A23V2250/2132A23V2250/21
Inventor 程妮曹炜王卫东
Owner 陕西康泰莱生物医药工程有限公司
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