Natural plant composition with sunscreen and anti-wrinkle effects

Abstract

The invention discloses a natural plant composition with sunscreen and anti-wrinkle effects. The natural plant composition comprises a scutellaria baicalensis root extract, an ormosia henryi bark extract and glucosylrutin. The combination of the scutellaria baicalensis root extract, the ormosia henryi bark extract and the glucosylrutin has a synergistic effect and can jointly resist ultraviolet radiation, and at least one of hinokiol, ferulic acid, phloretin and ellagic acid is added, so that the ultraviolet absorption effect of the natural plant composition can be further improved, and the natural plant composition can be used for preventing and treating ultraviolet radiation. The elasticity of the skin is improved, and wrinkles are reduced.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a natural plant composition with sunscreen and anti-wrinkle effects. Background Art

[0002] In recent years, people's awareness of sun protection has increased, and the demand for sun protection has further expanded. At the same time, people's requirements for the quality of sunscreen cosmetics are also constantly increasing. According to the sun protection mechanism, the sunscreens currently used are mainly divided into chemical sunscreens and physical sunscreens. Chemical sunscreens can absorb ultraviolet rays and convert them into other forms of energy for release, thereby reducing the ultraviolet energy irradiated to the skin, but they are prone to cause skin irritation, allergies, inflammation and other side effects. Physical sunscreens protect the skin by reflecting or scattering ultraviolet rays, but they are easy to clog pores and affect sweat gland secretion. As people's understanding of the toxic and side effects of existing sunscreens deepens, consumers' demands for returning to natural cosmetics are becoming stronger. In addition, under long-term ultraviolet irradiation, the collagen and elastic fibers in the skin are severely damaged, and the large number of free radicals (DPPH) generated will also lead to long-term inflammatory reactions. The superposition of the two phases causes the skin to lose elasticity, sag, turn yellow and have obvious wrinkles. Therefore, while avoiding damage caused by ultraviolet radiation, it is also necessary to replenish skin vitality and fight wrinkles. Summary of the invention

[0003] The purpose of the present invention is to provide a natural plant composition with sunscreen and anti-wrinkle effects.

[0004] To achieve the above object, the present invention adopts the following technical solution: One of the purposes of the present invention is to protect a natural plant composition with sunscreen and anti-wrinkle effects, which comprises scutellaria root extract, palmyra palm bark extract and glucosyl rutin.

[0005] Furthermore, in the natural plant composition, the mass ratio of the scutellaria root extract, the palm tree bark extract and the glucosyl rutin is (1-5):(5-15):(26-40).

[0006] Furthermore, the natural plant composition may also include at least one of hinokitiol, ferulic acid, phloretin and ellagic acid, and the content thereof accounts for 2%-20% of the total mass of the natural plant composition. Preferably, hinokitiol is used in combination with scutellaria root extract, glucosylrutin and palm tree bark extract.

[0007] The scutellaria root extract, the palm bark extract and the glucosylrutin all have good ultraviolet absorption capacity, and the combination of the three has a synergistic effect and can jointly resist ultraviolet radiation. Further adding cypress phenol, ferulic acid, phloretin and / or ellagic acid can significantly improve the sunscreen performance (SPF value and PA value) of the natural plant composition, remove the oxidative free radicals caused by ultraviolet rays in cells, and improve the collagen damage caused by ultraviolet radiation to the skin. In addition, the raw materials used are all natural plant ingredients and have little irritation to the skin.

[0008] The second purpose of the present invention is to protect the product with sunscreen and anti-wrinkle function prepared by using the natural plant composition with sunscreen and anti-wrinkle effect.

[0009] Furthermore, the product with sunscreen and anti-wrinkle functions includes skin care products or cosmetics, which can specifically be any one of essence, lotion, cream, facial mask, and liquid foundation.

[0010] Furthermore, the amount of the natural plant composition in the product is 0.15wt%~10wt%.

[0011] Furthermore, the product with sunscreen and anti-wrinkle functions also contains emulsifiers, moisturizers, film formers and other auxiliary materials that can be used in skin care products or cosmetics.

[0012] The composition of the present invention can play a significant benefit role in both water-in-oil and oil-in-water formulations.

[0013] Compared with the prior art, the present invention has the following beneficial effects: (1) The main components of the scutellaria root extract, the palm bark extract and the glucosylrutin used in the present invention are plant polyphenols, flavonoids and their derivatives, which have good antioxidant activity, can effectively remove DPPH caused by ultraviolet rays, and prevent skin oxidative damage.

[0014] (2) Pterostilbene is the main active ingredient of the bark extract of the rosewood tree. It has excellent antioxidant and UV absorption properties. It can improve the damage caused by UV radiation to skin collagen and elastin, increase skin elasticity, and reduce wrinkles. When used in combination with Scutellaria baicalensis root extract and glucosylrutin, the three ingredients have a synergistic effect and can significantly improve the UV absorption effect compared to a single ingredient.

[0015] (3) The cypress phenol, phloretin, ferulic acid, and ellagic acid added in the present invention can produce a synergistic effect with the scutellaria root extract, the palm bark extract, and the glucosylrutin, thereby enhancing the antioxidant activity and ultraviolet absorption capacity of the composition, and can relieve skin discomfort after sun exposure, greatly improving the sunscreen synergistic ability of the composition, thereby reducing the dosage of traditional sunscreens in the product, and avoiding the irritation and unsafe problems caused by the use of high-dose sunscreens. Therefore, the sunscreen spray, sunscreen cream, sunscreen gel, and other sunscreen products prepared with the plant composition as the main ingredient have good sunscreen function and good skin feel, and are non-irritating to the skin, will not cause skin allergies, and have no toxic side effects, and are highly safe to use.

[0016] (4) The cypress phenol, phloretin, ferulic acid and ellagic acid added in the present invention can effectively reduce the activity of elastase and elastic tissue damage, promote collagen production and replenishment, and reduce skin sagging. When used in combination with scutellaria root extract, palm bark extract and glucosylrutin, the anti-wrinkle and firming related indicators can be increased by more than 200%, and the damage of glycation products to skin cell activity can be inhibited to a certain extent. DETAILED DESCRIPTION

[0017] In order to make the contents of the present invention easier to understand, the technical solution of the present invention is further described below in conjunction with specific implementation methods, but the present invention is not limited thereto.

[0018] The present invention will be further described below in conjunction with embodiments: Unless otherwise specified, the reagents, methods and equipment used in the present invention are all conventional reagents, methods and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0019] The corresponding plant compositions were prepared according to the formula in Table 1. Then, the prepared plant compositions were used to prepare the corresponding products 1-7 and comparative products 1-2 with sunscreen functions, wherein product 1 used the plant composition of Example 1, comparative product 1 used the plant composition of Comparative Example 1, and the others were analogous.

[0020] Table 1 Sample formula

[0021] As an optional embodiment, the specific preparation method of the sunscreen product is as follows: (1) Add emollient, film former and emulsifier to the oil phase pot, stir and heat to 79-82°C, keep warm for later use; (2) Add water, humectant and thickener to the water phase pot, stir and heat to 79-82°C, stir and dissolve, and keep warm for later use; (3) The material in the oil phase pot is pumped into the water phase pot, homogenized for 2 to 8 minutes, stirred and cooled to 45 to 48°C, preservatives and natural plant composition are added, stirred for 5 to 15 minutes and then discharged.

[0022] The amounts of the raw materials in the sunscreen product are calculated by mass percentage as follows: 2% plant composition, 0.5-30% emollient, 0.1-5% film former, 3-12% humectant, 0.1-0.5% thickener, 0.5-2% preservative, 1-5% emulsifier, and the balance is water.

[0023] There are also products 8, 9 and comparative products 3 and 4. Among them, product 8 is the product 2 formula with 5% titanium dioxide sunscreen added, product 9 is the product 2 formula with 3% ethylhexyl triazone, 1% bis-ethylhexyloxyphenol methoxyphenyl triazine and 1% diethylamino hydroxybenzoyl hexyl benzoate added; comparative product 3 is the product 8 formula with the plant composition omitted, comparative product 4 is the product 9 formula with the plant composition omitted, and the remaining ingredients and processes are the same as those of product 2.

[0024] 1. Sunscreen performance test The SPF and PA values ​​of products 1-9 and comparative products 1-4 were tested using an ultraviolet transmittance analyzer UV-2000s. The sunscreen performance was compared based on the average SPF and PA values ​​of the samples. The higher the SPF or PA value, the better the sunscreen performance.

[0025] The specific operation is to fix the PMMA plate on the sample plate, and then use a spotter to 2 The sample is gently placed on a 5×5cm 2 The sample was placed on a PMMA plate of different sizes and evenly coated with a finger with a latex cot; the coated sample was placed in a dark environment at a temperature of (25±5)℃ and a relative humidity of (50±10)% to dry naturally for 20 minutes, and then 9 points were selected on the PMMA plate for testing using a UV-2000s analyzer to obtain the SPF, PA and standard deviation of a test. All data were measured in parallel 3 times and the average value was taken. The results are shown in Table 2.

[0026] Table 2 Sunscreen performance test results

[0027] From the results in Table 2, it can be seen that the sunscreen products 1-3 with added plant compositions have relatively stable sunscreen capabilities, with SPF values ​​all above 23 and PA values ​​all above 11. In contrast, the SPF values ​​and PA values ​​of comparative products 1 and 2 are lower, which proves that the combination of the components of the natural plant composition in the present invention has a certain synergistic effect, and this synergistic effect is closely related to the dosage thereof.

[0028] At the same time, the sun protection ability of products 4-7 with added cypress phenol, phloretin, ferulic acid or ellagic acid is significantly better than that of products 1-3 without addition, indicating that the use of cypress phenol, phloretin, ferulic acid or ellagic acid can produce a synergistic effect with Scutellaria baicalensis root extract, Pterocarpus ovata bark extract and glucosylrutin, enhancing the ultraviolet absorption ability of the natural plant composition. Among them, product 4 prepared with Scutellaria baicalensis root extract, Pterocarpus ovata bark extract, glucosylrutin and cypress phenol in a ratio of 3:10:33:1 has the best sun protection effect.

[0029] In addition, the SPF value of product 2 is 28.56, and the PA value is 14.34. The SPF values ​​of products 8 and 9 are greater than 50, and the PA values ​​are greater than 20. The SPF values ​​of comparison products 3 and 4 are 16.73 and 16.22, and the PA values ​​are 5.51 and 4.54, respectively. This shows that the natural plant composition of the present invention can be used in combination with traditional sunscreens to coordinately improve the sun protection ability of the products.

[0030] 2. Anti-wrinkle performance test The anti-wrinkle performance of the plant compositions of Examples 2, 4-7 was verified. Before the experiment, 0.2 mg of the plant composition was dissolved in 1.8 mg of dimethyl sulfoxide (DMSO) to prepare a 10 wt % stock solution, which was diluted to a 0.15 wt % sample test solution when used.

[0031] 1) DPPH free radical scavenging performance Prepare DPPH solution with anhydrous ethanol to obtain a DPPH solution of appropriate concentration. Take 2 mL of sample test solution and mix it with 2 mL of DPPH solution. After reacting at room temperature and in the dark for 30 minutes, use a UV spectrophotometer to detect the absorbance at a wavelength of 517 nm (repeat the measurement 3 times and take the average value), and calculate the DPPH removal rate of the sample according to the following formula. The results are shown in Table 3: DPPH free radical scavenging rate (%) = {1-(AC) / B} × 100%, Among them, A is the absorbance value of 2 mL sample test solution + 2 mL DPPH solution, B is the absorbance value of 2 mL DPPH solution + 2 mL DMSO, and C is the absorbance value of 2 mL anhydrous ethanol + 2 mL sample test solution.

[0032] Table 3 Test results of DPPH free radical scavenging performance

[0033] As can be seen from Table 3, the DPPH free radical scavenging rate of each plant composition in the examples is relatively high, indicating that the natural plant composition of the present invention has good antioxidant capacity, and the addition of cypress phenol, phloretin, ferulic acid or ellagic acid can further enhance the free radical scavenging capacity of the composition, among which Example 4 with the addition of cypress phenol has the best effect.

[0034] 2) Inhibitory properties of inflammatory factor α tumor necrosis factor (TNFα) after UV irradiation Add 100 μL of mouse mononuclear macrophage leukemia cell culture medium and 100 μL of sample test solution to a 96-well plate, mix well and incubate in a 25 °C incubator for 20 min. 2 The cells were irradiated with UVB for 2 h. After the irradiation, the culture medium was discarded after incubation for 24 h. Total RNA was extracted and reverse transcribed using a reverse transcription kit in a common PCR amplification instrument to synthesize cDNA. The absorbance was detected at a wavelength of 490 nm, and the inhibition rate of the inflammatory factor α tumor necrosis factor (TNFα) in the sample was calculated according to the following formula. The results are shown in Table 4: TNFα inhibition rate (%) = (control group - experimental group) / control group × 100%, In the control group, an equal amount of DMSO was used to replace the sample diluent.

[0035] Table 4 Test results of TNFα inhibitory performance after ultraviolet irradiation

[0036] It can be seen from Table 4 that each plant composition in the examples has a good inhibitory effect on the inflammatory factor TNFα after ultraviolet irradiation, indicating that the natural plant composition of the present invention has excellent post-sun inflammation inhibition function, and the addition of cypress phenol, phloretin, ferulic acid or ellagic acid helps to improve the post-sun inflammation inhibition function of the composition, among which Example 4 with the addition of cypress phenol has the best effect.

[0037] 3) Inhibition of elastase activity According to the formula in Table 5, phosphate buffer solution, DMSO, sample diluent, substrate solution (N-succinyl-L-alanyl-L-alanyl-L-alanine-p-nitroaniline dissolved in DMSO) and elastase were added to a 96-well plate, and the plate was shaken for 5 minutes to ensure that the reaction solution was fully mixed. The reaction was then placed in a 37°C constant temperature incubator for 30 minutes. The absorbance of each well was measured at a wavelength of 405 nm using an enzyme reader, and the elastase inhibition rate of the sample was calculated according to the following formula. The results are shown in Table 6: Elastase inhibition rate (%) = {1-(T2-T1) / (C2-C1)}×100%, Wherein, T1 is the absorbance value of the control sample, T2 is the absorbance value of the sample, C1 is the absorbance value of the blank control, and C2 is the absorbance value of the blank.

[0038] Table 5 Reaction solution formula

[0039] Table 6 Test results of elastase activity inhibition performance

[0040] It can be seen from Table 6 that each plant composition in the examples has a high elastase inhibition rate, indicating that the natural plant composition of the present invention has a good effect in slowing down the degradation of elastin, and the addition of cypress phenol, phloretin, ferulic acid or ellagic acid can improve the effect of the composition in slowing down the degradation of elastin, among which Example 4 with the addition of cypress phenol has the best effect.

[0041] 4) Collagen secretion experiment The sample was tested according to the instructions of the human type I collagen enzyme-linked immunosorbent assay kit. The specific operation is to add the sample dilution dropwise to the human skin fibroblasts for reaction for 24 hours, take the supernatant after centrifugation and immediately add it to the enzyme-labeled wells pre-coated with antibodies, then add the recognition antigen labeled with horseradish peroxidase (HRP), incubate at 37°C for 1 hour, measure the absorbance at a wavelength of 450nm, and draw a standard curve. Then, use the standard curve to calculate the type I collagen content of the cells after sample stimulation, and calculate the type I collagen enhancement rate of the sample according to the following formula. The results are shown in Table 7: Type I collagen enhancement rate (%) = (experimental group - control group) / control group × 100%, In the control group, an equal amount of DMSO was used to replace the sample diluent.

[0042] Table 7 Results of type Ⅰ collagen secretion experiment

[0043] As can be seen from Table 7, the type I collagen enhancement rate of each plant composition in the examples is relatively high, indicating that the natural plant composition of the present invention has a good effect on enhancing the secretion of collagen I, and the addition of cypress phenol, phloretin, ferulic acid or ellagic acid can improve the effect of the composition in promoting the secretion of collagen I, among which Example 5 with the addition of ferulic acid has the best effect, and Example 4 with the addition of cypress phenol has the second best effect.

[0044] 5) Glycosylation end products AGEs inhibition experiment According to the formula in Table 8, AGEs reaction solution (2.5g bovine serum albumin + 0.9g glucose was added to 50mL phosphate buffer solution and reacted in a 50℃ incubator for more than 100h), sample diluent, phosphate buffer solution and deionized water were added to a 96-well plate, mixed and reacted at 50℃ for 3h, and the fluorescence value of each well was measured by an ELISA instrument at a wavelength of λex / λem=360nm / 446nm, using a blue filter to avoid light, and the AGEs inhibition rate of the glycosylated protein end products of the sample was calculated according to the following formula. The results are shown in Table 9: AGEs inhibition rate (%) = {1-(T1-T2) / (C1-C2)}×100, Wherein, T1 is the absorbance value of the sample, T2 is the absorbance value of the control sample, C1 is the absorbance value of the blank, and C2 is the absorbance value of the blank control.

[0045] Table 8 Reaction solution formula

[0046] Table 9 Inhibition test results of glycosylation protein end products AGEs

[0047] As can be seen from Table 9, the inhibition rates of the plant compositions in the examples on the end products of glycation proteins, AGEs, are relatively high, indicating that the natural plant compositions of the present invention have a certain effect of inhibiting the damage of glycation products to skin cells, and the addition of phytosan, phloretin, ferulic acid or ellagic acid can enhance the inhibitory effect of the composition on the end products of glycation proteins, AGEs. Among them, Example 7 with the addition of ellagic acid has the best effect, and Example 4 with the addition of phytosan has the second best effect.

[0048] From the above, it can be seen that the combination of cypress phenol with scutellaria root extract, tamarisk bark extract and glucosylrutin can significantly improve the sunscreen performance of the composition, and shows the best effect in scavenging DPPH free radicals, inhibiting inflammatory factors and elastase activity, and also performs well in the performance tests of promoting collagen production and inhibiting glycation end products. Therefore, cypress phenol can be used as the preferred combination active ingredient with scutellaria root extract, tamarisk bark extract and glucosylrutin.

[0049] 3. Security Testing Multiple skin irritation tests were conducted on sunscreen products 1-9 for 14 days to test their safety. The test methods in Chapter 6 of the "Technical Specifications for Safety of Cosmetics 2015 Edition" were used. The test results are shown in Table 10.

[0050] Table 10 Results of multiple skin irritation tests

[0051] As can be seen from Table 10, the sunscreen products prepared using the plant composition alone or in combination with traditional sunscreen agents have no skin irritation, indicating that the plant composition is relatively safe to use.

[0052] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A natural plant composition with sunscreen and anti-wrinkle effects, characterized in that: The natural plant composition comprises scutellaria baicalensis root extract, palmyra palm bark extract and glucosyl rutin.

2. The natural plant composition with sunscreen and anti-wrinkle effects according to claim 1, characterized in that: The mass ratio of the scutellaria root extract, the palm tree bark extract and the glucosylrutin used in the natural plant composition is (1-5):(5-15):(26-40).

3. The natural plant composition with sunscreen and anti-wrinkle effects according to claim 1, characterized in that: The natural plant composition also includes at least one of cypress phenol, ferulic acid, phloretin and ellagic acid, and the content thereof accounts for 2%-20% of the total mass of the natural plant composition.

4. A product with sunscreen and anti-wrinkle functions prepared using the natural plant composition according to claim 1 or 3.

5. The product with sunscreen and anti-wrinkle functions according to claim 4, characterized in that: The amount of the natural plant composition in the product is 0.15wt%~10wt%.

6. The product with sunscreen and anti-wrinkle functions according to claim 4, characterized in that: The products include skin care products or cosmetics.

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