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Application of 8-hydroxydeoxyguanosine as urine marker

A hydroxydeoxyguanosine and marker technology, which is applied in the field of urine markers for early screening of colorectal cancer, can solve the problems of high missed detection rate of fecal occult blood, visual and psychological burden of testers, etc. Effects that are easy to acquire, easy to collect

Inactive Publication Date: 2017-02-01
ZHEJIANG UNIV
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Problems solved by technology

However, the missed detection rate of fecal occult blood is very high, and not all patients with bleeding are colorectal cancers, and the appearance of false positives will increase the pain of the individual under examination for colonoscopy and other examinations
In addition, feces can be visually and psychologically taxing for testers

Method used

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  • Application of 8-hydroxydeoxyguanosine as urine marker
  • Application of 8-hydroxydeoxyguanosine as urine marker
  • Application of 8-hydroxydeoxyguanosine as urine marker

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Embodiment 1

[0032] In the present invention, the body fluid sample provided for determination of 8-hydroxydeoxyguanosine is urine.

[0033] A. The pretreatment of the urine sample before the measurement is described in step A. First, the urine is centrifuged at 13000r / min for 15min at 4 degrees Celsius and then 0.5ml of the supernatant is taken, diluted with water to 1ml. Add 10 pmol of isotopic internal standard.

[0034] B. What is described in step B is to carry out solid phase extraction pretreatment to urine. The solid phase extraction is to add 1.0ml methanol to the HLB Oasis column for activation, then add 1.0ml water for equilibrium, and then add urine sample. It was then washed with 1.0 ml of 5% methanol, and finally eluted with 1 ml of 50% methanol. The eluate was collected and dried with a vacuum centrifuge, then reconstituted with 0.2ml of water for testing.

[0035] C. In step C, the urine to be tested after the pretreatment is separated by ultra-high performance liquid ch...

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Abstract

The invention provides an application of 8-hydroxydeoxyguanosine as a urine marker. The molecular formula of the 8-hydroxydeoxyguanosine is C10H13O5N5. In detection, collection of a sample is non-invasive, and the sample is easy to obtain and is large in acquisition quantity. Compared with FOBT test, the method is more acceptable for detectors and can reduce mental burden of work staff, and is more acceptable for common people. By means of optimized off-line solid-phase extraction technology with ultrahigh performance liquid chromatography-tandem mass spectrometry, the concentration of a DNA oxidative damage marker, 8-hydroxydeoxyguanosine, in urine of a to-be-tested subject can be accurately quantified, so that disease risk analysis to the subject can be carried out through an established colorectal cancer incidence prediction model. The urine marker, when being used for screening the colorectal cancer, is non-invasive, quick and high-flux. The detection method not only is suitable for early stage detection of the colorectal cancer but also is suitable for detection of metastasis or non-metastasis of the colorectal cancer. The application has excellent prospect.

Description

technical field [0001] The invention belongs to the technical field of detection, relates to malignant tumor detection technology, in particular to a urine marker for early screening of colorectal cancer and its application, which is a new non-invasive screening method for in vitro detection of colorectal cancer Early detection and detection. Background technique [0002] Colorectal cancer is a common malignant tumor and one of the fastest-rising tumors in recent years. The worldwide incidence rate is the third in men and the second in women. With the development of economy, the improvement of living standard and the change of lifestyle, its incidence rate will also show a rising trend. Although the diagnosis and treatment of colorectal cancer have made great progress in recent years, the overall curative effect of colorectal cancer has not improved significantly, which is mainly because more than half of the patients who visit the hospital are in the advanced stage. The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 郭成丁培丽李晓芬王蓉余捷凯张苏展郑树
Owner ZHEJIANG UNIV
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