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Application of shippocampus extract 2H5M in preparation of drug for preventing and treating neurodegenerative diseases and health product of drug

A neurodegenerative, 2H5M technology, applied in nervous system diseases, neuromuscular system diseases, antipyretics, etc.

Pending Publication Date: 2018-11-16
深圳市万骐海洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are no studies on the effects and mechanisms of hippocampal extract 2H5M in inhibiting the excessive activation of microglia and using it to treat neurodegenerative diseases

Method used

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  • Application of shippocampus extract 2H5M in preparation of drug for preventing and treating neurodegenerative diseases and health product of drug
  • Application of shippocampus extract 2H5M in preparation of drug for preventing and treating neurodegenerative diseases and health product of drug
  • Application of shippocampus extract 2H5M in preparation of drug for preventing and treating neurodegenerative diseases and health product of drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Preparation of hippocampus extract 2H5M

[0037] The compounds used in this experiment were isolated from Hippocampus kudaBleeler in the sea area of ​​Zhoushan, Zhejiang Province, and the identification was completed by Professor Deng Mingdeng, a zoologist at Changchun University of Traditional Chinese Medicine in China. The extraction and identification of compounds such as figure 1 shown. The structural formula of 2H5M is as follows:

[0038]

Embodiment 2

[0039] Example 2: Recovery, passage and cryopreservation of mouse microglioma cells (BV-2)

[0040] 2.1 Cell Recovery

[0041] Take out a cryopreservation tube of frozen BV-2 cells from the liquid nitrogen tank, immediately put it into a water bath at 37°C and shake it constantly. Transfer 1 mL of cryopreservation solution to a cell culture bottle filled with 20 mL of complete medium in an ultra-clean workbench, and place it in a cell culture incubator for culture. After 4 hours, the culture flask was taken out, the cell morphology was observed and the medium was changed, and the culture was continued.

[0042] 2.2 Cell passage

[0043] Take out the cell culture bottle from the incubator and put it into the ultra-clean bench, pour off the culture medium in the culture bottle, add 1mL PBS to wash the cell surface, repeat twice; then add 1mL trypsin to digest, shake the culture bottle gently Infiltrate to the cell surface. Observe the digestion status under a microscope. Whe...

Embodiment 3

[0046] Embodiment 3: MTT colorimetric experiment

[0047] 3.1 Experimental principle

[0048] MTT colorimetry is a method used to detect cell viability and growth. The principle is: succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT (3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide) to Formazan forms water-insoluble blue-violet crystals and deposits in cells, but dead cells do not have this function. Dimethyl sulfoxide (DMSO) can dissolve formazan in the cells and generate a purple solution, and use a microplate reader to measure the absorbance at OD540nm wavelength. The amount of MTT formed is directly proportional to the total number of living cells.

[0049] 3.2 Experimental steps

[0050] 100 μL of BV-2 cell suspension (obtained from Example 2) was added to each well of a 96-well plate, and cultured in an incubator. After 24 hours, the old culture medium was discarded, and culture medium containing sample concentrations of 1...

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Abstract

The invention relates to application of a shippocampus extract 2H5M in preparation of a drug, a health product or a dietary supplement for preventing and treating neurodegenerative diseases. The chemical name of the shippocampus extract 2H5M is 2'-hydroxyl-5'-methoxyacetophenone. The shippocampus extract 2H5M is from hippocampus kuda bleeler, does not have cytotoxicity to mouse microglia, can inhibit generation of NO and ROS in the mouse microglia, and can lower DNA oxidative damage.

Description

technical field [0001] The invention relates to the use of hippocampus extract 2H5M in the preparation of medicines, health products or dietary supplements for preventing and treating neurodegenerative diseases. Background technique [0002] Neuroinflammation is a key defense mechanism against infectious agents and neuronal damage in the central nervous system, however, excessive neuroinflammation leads to neuronal damage, which eventually leads to neurodegenerative diseases (such as Parkinson, Alzheimer, amyotrophic lateral cable hardening). Although the hypothesis of inflammation as the pathogenesis of neurodegenerative diseases is still controversial, there is a consensus that the persistence of local inflammation can lead to neuronal damage and accelerate the development of neurodegenerative diseases. Microglia are macrophage-like cells resident in the central nervous system, which play a role in regulating nerve homeostasis and injury repair. However, under inflammato...

Claims

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Application Information

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IPC IPC(8): A61K31/12A61P25/28A61P25/16A61P21/00A61P25/02A61P29/00A61P39/06A23L33/10
CPCA61P21/00A61P25/02A61P25/16A61P25/28A61P29/00A61P39/06A23L33/10A61K31/12A23V2002/00A23V2200/322A23V2200/30A23V2250/2042
Inventor 千忠吉张嫄嫄崔玉华刘梓韬
Owner 深圳市万骐海洋生物科技有限公司
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