8 results about "Methyl cytosine" patented technology

The present disclosure is concerned with modified proteins, methods, compositions, and kits for mapping of methylation status of nucleic acids, including 5-methylcytosine. In some embodiments, the proteins are altered cytidine deaminases, such as altered APOBEC3A proteins. In some embodiments, the proteins selectively act on cytosines and have significantly reduced activity on modified cytosines, such as 5-methylcytosine.

The present disclosure is concerned with modified proteins, methods, compositions, and kits for mapping of methylation status of nucleic acids, including 5-methylcytosine and 5-hydroxymethyl cytosine (5hmC). The proteins selectively act on certain modified cytosines of target nucleic acids and include one or more substitution mutations that enhance the selectivity of the proteins for certain modified cytosines, enhance the stability of the proteins, or enhance both selectivity and stability. Also provided are compositions and kits that include one or more of the proteins and methods for using one or more of the proteins.

The application provides a nucleic acid aptamer-drug conjugate, a preparation method thereof and application thereof in preparing an anti-tumor drug, and belongs to the technical field of biological medicines. The nucleic acid aptamer-drug conjugate comprises a nucleotide sequence, an HG9 nucleic acid aptamer as shown in SEQ ID NO:1 and an extended chain at the end of the HG9 nucleic acid aptamer; the extended chain is loaded with 5-hydroxymethylcytosine and / or 5-formylcytosine. The application further provides a preparation method of the nucleic acid aptamer-drug conjugate. By means of PCR amplification technology, an epigenetic modification nucleoside with cytotoxicity is introduced into a nucleic acid aptamer with specific targeting capability, the nucleic acid aptamer-drug conjugate constructed has the binding capability to target cells, and can release the modified nucleoside in the cells, cause DNA damage and significantly inhibit the activity of the target cells. The method is simple in operation and high in efficiency, and the obtained conjugate has potential value in drug development.

The present invention provides methylcytosine selective deaminases, compositions, kits, and methods of using the thereof, including sequencing methods involving: (a) contacting a DNA substrate comprising cytosine (C) and at least one methylcytosine nucleobase selected from 5-methylcytosine (5mC) and 5-hydroxymethylcytosine, or comprising C and both 5mC and 5hmC, with a methylcytosine selective deaminase to produce a deamination product, wherein the methylcytosine selective deaminase (i) is capable of deaminating 5mC to thymidine (T) and / or deaminating 5hmC to hydroxymethyluridine (hmU), and (ii) preferentially deaminating at least one methylcytosine nucleobase relative to cytosine (C); and (b) sequencing the deamination product, or amplifying the deamination product to produce an amplification product, and sequencing the amplification product to produce a sequence read in each case, wherein the position of C and the position of at least one methylcytosine nucleobase in the DNA substrate are determined based on the sequence read.

Provided herein are methods of inhibiting a respiratory virus (i.e., a virus associated with a respiratory condition, such as influenza A virus, influenza B virus, RSV, etc.). Aspects of the methods include contacting a sample comprising viral RNA (vRNA) having a target motif with an effective amount of an agent that specifically binds the target motif to inhibit the respiratory virus. Also provided are methods of treating or preventing a respiratory virus infection in a subject. Also provided are compounds and pharmaceutical compositions comprising an oligonucleotide sequence complementary to a target vRNA region, which can be used in the subject methods. The provided oligonucleotides can comprise 5-methylcytosine.

This disclosure provides compositions for use in the preparation of target nucleic acid molecules, comprising one or more 5-formylcytosine bases or adducts of 5-formylcytosine. Also disclosed herein is a method for efficiently synthesizing nucleic acid molecules comprising one or more 5-formylcytosine bases from target nucleic acid molecules comprising one or more 5-hydroxymethylcytosine bases. This disclosure also provides a method for detecting epigenetic modifications in target nucleic acid molecules, such as epigenetic modifications characterized by methylation of cytosine at position 5 (e.g., 5-methylcytosine; 5-hydroxymethylcytosine).

Provided herein are methods and systems related to obtaining and processing sequence reads. The methods may comprise obtaining cell-free DNA sequences from a subject and obtaining sequencing reads from the cell-free DNA sequences that identify cell-free DNA sequences, 5-methylcytosines, and 5-hydroxymethylcytosines. The methods may involve applying a trained classifier to the sequencing reads.

The present disclosure is concerned with modified proteins, methods, compositions, and kits for mapping of methylation status of nucleic acids, including 5-methylcytosine and 5-hydroxymethyl cytosine. In some embodiments, the modified proteins have been altered to increase protein stability. In some embodiment, the proteins selectively act on certain modified cytosines of target nucleic acids and include one or more substitution mutations that enhance the selectivity of the proteins for certain modified cytosines, optionally enhance the stability of the proteins, or optionally enhance both selectivity and stability. Also provided are compositions and kits that include one or more of the proteins and methods for using one or more of the proteins.