42results about How to "Immunocompetent" patented technology

A method for preparing a goat milk beverage containing ACE (Angiotensin Converting Enzyme) inhibitory peptide comprises the following steps of: cooling, centrifuging and fatting reconstituted goat milk, thereby obtaining skimmed milk; preparing casein from the skimmed milk by an isoelectric precipitation method, and obtaining polypeptide powder through enzymolysis; and mixing polypeptide powder with reconstituted goat milk, concentrated fruit juice, polypeptide powder, cane sugar, citric acid, beta-cyclodextrin, purified water and the like to obtain the goat milk beverage containing ACE inhibitory peptide. The goat milk beverage containing ACE inhibitory peptide prepared by the method provided by the invention contains the ACE inhibitory peptide and thereby has obvious effect of reducing blood pressure; if the hyperpietic drinks the goat milk beverage for a long time, the blood pressure of the hyperpietic can be reduced; and the goat milk beverage is mild in properties without side effect on people of normal blood pressure; and the goat milk beverage containing ACE inhibitory peptide has various effects of immunological activity, and prompting digestive absorption and losing weight, and the like.

The invention relates to the technical field of extraction of active ingredients of edible mushrooms, and particularly relates to a method for simultaneously extracting polysaccharide and polyphenol from needle mushrooms. The method comprises the steps: preparing an extraction solution, extracting an alkaline solution by assistance of ultrasonic wave, performing enzymolysis on residue, extracting polyphenol from ethyl acetate and extracting polysaccharide after ultrafiltration and desalination. According to the method, operations such as auxiliary extraction, enzymolysis and membrane filtration are organically combined, and the polysaccharide and the polyphenol are simultaneously extracted, so that the production process is simplified, the safety and reliability are achieved, the yield is high, the physiological and pharmacological activities of the polysaccharide and the polyphenol in a needle mushroom sporocarp are well reserved, and a larger economic value is achieved.

The invention relates to a temperature-sensitive hydrogel having bioactivity and a preparation method of the temperature-sensitive hydrogel. The preparation method comprises the following steps: 1) preparing HO-PEG-COOH; 2) preparing Ac-PEG-COOH; 3) preparing Ac-PEG-Ac; 4) preparing Ac-PEG-g-IKVAV; 5) preparing a PNIPAM-b-PEG-g-IKVAV precursor; and 6) preparing the temperature-sensitive hydrogel having the bioactivity, namely dissolving the PNIPAM-b-PEG-g-IKVAV precursor into DMF, and then adding PNIPAM, a micromolecular cross-linking agent, Ac-PEG-Ac and AIBN, sealing and vacuumizing and then reacting at 70 DEG C for 12 hours. The preparation method is simple in process; the obtained hydrogel is quick in response to temperature change response to temperature change, has bioactivity and is capable of promoting the adhesion and proliferation of nerve cells; as a result, a support materials is provided for central nervous restoration.

The invention provides a method for preparing nano-silver composite particles from emblica polysaccharide and belongs to the technical field of nanometer materials. The method comprises the steps of preparation of emblica polysaccharide and preparation of nano-silver. According to the prepared composite particles, the diameter, measured through bacteria inhibition experiments, of inhibition zones is 27.9+/-0.01 mm; a theoretical value and an experimental value are basically equal. This shows that the process for preparing AgNPs through a response surface optimization method is green and reliable.

An agaricus bisporus polysaccharide with immunocompetence is a heteropolysaccharide consists of ribose, rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 2.08:4.61:2.45:22.25:36.45:89.22:1.55. The agaricus bisporus polysaccharide is prepared by extracting agaricus bisporus in a water bath; precipitating the extract with ethanol; deproteinizing the precipitate to obtain coarse polysaccharide; processing the coarse polysaccharide by ion exchange column chromatography with an anion exchange packing; eluting with distilled water; carrying out freeze drying; carrying out gel chromatography; eluting with distilled water; carrying out freeze drying to obtain the final product. The agaricus bisporus polysaccharide has immunological enhancement effect, and can serve as adjuvant drug for treating immunodeficiency diseases, such as cancer, or serve as functional health products and foods for enhancing immunity.

The invention provides a method for testing non-ethylating CpG content, which comprises adopting specific methylaseSssI to modify CpG dinucleotide aracytidine into 5-methylcytosine, then using nucleicacidase P1 and bacterial alkaline phosphatase to hydrolyze DNA into single deoxynucleoside, using a reversed-phase hplc method to test quantity of the 5-methylcytosinein DNA hydrolytic samples which are both modifying and un-modifying, and testing the CpG through differences of the 5-methylcytosine detectable amount between the modifying DNA hydrolytic sample and the un-modifying DNA hydrolytic samples.

The invention discloses a preparing method for low-irritability infant nutritive rice flour. The preparing method includes the steps that organic rice serves as a main raw material, and smashing, screening, pulp mixing, stirring and ultrasonic treatment are conducted; a pH value of pulp is adjusted, trypsin and neutral protease are added, centrifugation is conducted, supernatant is filtered with an ultrafiltration membrane, a solution being smaller than 10 kDa and centrifugal solid are mixed and stirred, the pH value of the pulp is adjusted again, and mesophilic alpha-amylase is added for enzymolysis; the mixture is subjected to roller drying to obtain rice slices, cured grain powder, fruit powder, whole milk powder, soy isolate protein, concentrated whey protein, maltodextrin, fructo-oligosaccharide, galactooligosaccharide, probiotics, mineral substances and multivitamins are added, and are ground and mixed evenly, and sterilization and filling are conducted. The low-irritability infant nutritive rice flour prepared with the method is used for infants of 6 months or above to eat, the problems that an ingredient is single, functional ingredients are insufficient, polypeptide is bitter, the taste is poor, and digestion is difficult are solved, the immunity can be improved, sensitization is reduced, and the rice flour has wide application in the infant food field.

The invention particularly discloses a liquid feed additive and a preparation method thereof. Every 1000g of the liquid feed additive is prepared by mixing the following components: 0.5 to 2.0 g of vitamin B1, 0.8 to 2.5 g of vitamin B2, 0.3 to 1.5 g of vitamin B6, 15 to 30 g of vitamin C, 0.05 to 0.1 g of folic acid, 3 to 6 g of nicotinamide, 10 to 15 g of taurine, 40 to 60 g of tween-80 and thebalance of plant leaf fermentation liquor. According to the invention, the defects of low application and utilization rate, incapability of effectively utilizing nutritional value, poor functionalityand the like of plant leaves serving as a feed additive are overcome, and an effective way is found for efficient utilization of plant leaves in the field of animal feeds and development of utilization of waste resources. The product provided by the invention can be applied to livestock and poultry compound feed total mixed ration according to an addition amount of 1%, and can also be blended intowater according to an addition amount of 0.5% to be used as a livestock and poultry drinking water agent.

The invention provides a feed rich in probiotics and active peptide and an application of the feed and belongs to the field of feed production. The feed rich in the probiotics and the active peptide is fermentation product of a mixed solution of a mixed material formed by corn gluten meal and bran and water under the common fermentation action of bacillus licheniformis and lactobacillus, wherein the mass concentration of the active peptide in the feed is 94-100mg/g; the cell concentration of the probiotics in the feed is (0.5-2.0)*10<9>CFU/g; and bacillus licheniformis is firstly inoculated into a feed liquid for separate fermentation before fermentation and lactobacillus is inoculated into the feed liquid for common fermentation. According to the feed, the palatability of the feed can be significantly improved and the food intake of animals is improved, so that the production performance of the animals is improved. Meanwhile, the immune performance of the animals can be improved by the feed.

The invention discloses a beta-glucan gene vector, and a preparation method and application thereof. The preparation method comprises the following steps: (1) dissolving beta-glucan in a phosphate buffer solution, and heating at 130-145 DEG C for 15-60 minutes or dissolving in a dimethylsulfoxide or strongly alkaline solution to prepare a denatured single-chain polysaccharide solution; (2) bonding poly(dA)n with target nucleotide through a covalent bond, and dissolving in a buffer solution with the pH value of 7-9; and (3) adding the denatured single-chain polysaccharide solution in the step (1) into the poly(dA)n-target nucleotide/buffer solution, evenly mixing, and standing for 12-48 hours. The gene vector does not have cytotoxicity, has the advantages of favorable stability, higher transfection efficiency and higher immunocompetence, can avoid the inhibiting action of the serum due to the negative charge, can be widely used for CpGDNA transfection, can promote the release of inflammation regulatory factors, and can be used for immunotherapy.

The invention discloses an anti-tumor composition containing immune cells. The composition contains a dendritic cell culture, a T lymphocyte culture, a rosemary herb extract, a daffodil flower extractand a blueberry extract. The anti-tumor combination inhibits tumor growth through the mode that dendritic cells (DC) and T cells are in contact to stimulate an immune response. The dendritic cells (DC cells) express MHC type-I and type-II molecules at a high level, and when the DC cells expressing tumor-related peptide antigens and MHC type-I molecules activate CD4 and CD8 T cells, the T cells can exert effects to kill tumor cells. The composition contains the rosemary herb extract, the daffodil flower extract and the blueberry extract which all have an anti-tumor effect. The anti-tumor composition can protect cells against damage caused by external environmental factors while inhibiting tumors, and the tumors are prevented and resisted.

The invention discloses an anti-inflammatory probiotic solid beverage for children, and belongs to the technical field of nutritious food. The anti-inflammatory probiotic solid beverage is prepared from lactobacillus acidophilus, lactobacillus rhamnosus, bifidobacterium bifidum, bifidobacterium adolescentis, bifidobacterium breve, lactobacillus plantarum, oligomeric isomaltose, galactooligosaccharide, ginseng, licorice roots, dandelions, smoked plums, shiitake mushrooms, egg yolk globulin powder, pea peptide powder, yeast beta-glucan, acerola cherry powder, xylitol and skim milk powder; the ginseng, the licorice roots, the dandelions, the smoked plums and the shiitake mushrooms are extracted by water, and are made into the solid beverage after being dried and mixed with other raw materials. The anti-inflammatory probiotic solid beverage has the advantages that the immunity of human bodies is improved greatly, adverse reactions brought by inflammatory factors to the bodies are eliminated, and the resistivity of the bodies is improved; the anti-inflammatory probiotic solid beverage can diminish inflammation, detoxify the bodies and regulate gastrointestinal functions, thereby being especially suitable for children to drink.

The invention provides a recombinant plasmid, a recombinant baculovirus prepared by from the recombinant plasmid and application of the virus in preparation of tertian malaria transmission blocking vaccines. The recombinant vector is constructed by inserting a section of recombinant sequence into the pFastBacDual plasmid. The recombinant sequence is formed by: according to CMV, Pph, SP and TM known sequences, adding Xba I enzyme site and Xho I enzyme site between SP and TM sequences, adding KpnI enzyme site in front of CMV-F, and adding HindIII enzyme site after TM-R. The recombinant baculovirus is obtained by: inserting a Pvs25 gene into the recombinant plasmid, conducting homologous recombination with the genome of a shuttle vector Bacmid through transposition, then transfecting a bombyx mori cell, and performing packaging in the bombyx mori cell. The vaccine production method has the characteristics of good safety, low production cost, and easy mass production operation.

The invention discloses recombinant escherichia coli capable of synthesizing a bordetella pertussis oligosaccharide antigen, and belongs to the field of genetic engineering and synthetic biology. An O-antigen gene cluster wbbL-galF, a core sugar gene cluster rfaD-waaQ, an intestinal common antigen gene cluster rfe-rffM, a claic acid gene cluster wcaM-wza and a transcription binding factor metJ gene on an escherichia coli MG1655 genome are knocked out, core sugar of a pertussis CS strain, trisaccharide of the pertussis CS strain and a gene cluster for controlling the length of a trisaccharide unit are over-expressed, and a recombinant bacterium MDCO020/pWpBpD5 is obtained. Under the same fermentation condition, the synthetic cell dry weight of the MDCO020/pWpBpD5 strain is 1.63 times that of the CS strain; and the yield of the oligosaccharide antigen of the MDCO020/pWpBpD5 strain is two times that of the CS strain.

The invention discloses a hericium erinaceus polysaccharide chelated zinc microcapsule and a preparation method thereof. The method includes utilizing a hericium erinaceus polysaccharide as a raw material, chelating the hericium erinaceus polysaccharide with zinc ions to form a polysaccharide zinc chelate, performing homogenization emulsification and spray drying with carrageenan: chitosan: beta-cyclodextrin as wall materials and performing microencapsulation treatment to obtain the hericium erinaceus polysaccharide chelated zinc microcapsule. The microcapsule overcomes shortcomings of poor antioxidant activity of the traditional hericium erinaceus polysaccharide and low zinc content in the hericium erinaceus polysaccharide chelated zinc (Zn-HEP), can not only improve the dispersibility and the emulsifying dispersibility, but also protect the easily oxidized components in the polysaccharide, enhance stability and antioxidant activity and prolong the shelf life. The prepared hericium erinaceus polysaccharide chelated zinc microcapsule has obvious anti-oxidation activity, and enhances immune function and anti-cancer effect. The preparation method is simple and convenient, capable ofachieving industrial continuous production, and has wide practical value.

The invention relates to bean residue dietary fiber active peptide face-beautifying low-alcohol-content rice wine and a preparation method thereof. The rice wine is characterized by being prepared with, by weight, 200-300 parts of fresh bean residue, 30-35 parts of rice bran protein, 100-250 parts of rice wine, 5-7 parts of kudzu vine root extract, 3-5 parts of licorice extract, 5-7 parts of rose apple seed extract and 10-13 parts of perilla oil. Through the steps of drying, smashing, pickling, fermenting and enzymolyzing bean residue, beany flavor of the rice wine is reduced, and the rice wine is better in taste and more exquisite in quality and is fully utilized. A new drinking concept of seeking stimulus while pursing nutrition and health during drinking of people is met, a new nutrition absorbing concept of direct absorption is provided for people, improving of immunity is facilitated, and the rice wine has functions of resisting oxidation, lowering blood pressure and blood fat and protecting liver and is incomparable for any non-peptide wine.

The invention discloses a Maitake proteoglycan sugar-reducing compound food. The product is prepared by compounding Maitake proteoglycan dry powder, agaricus blazei water extract, coprinus comatus water extract and bitter melon freezing dry powder. The product has effect for non-insulin-dependent diabetes mellitus, can lead the patient with diabetes mellitus to eliminate the side effects for taking chemical sugar-reducing drugs for long period, improves the life quality of the patient with diabetes mellitus and is suitable for eating for long period.

The invention discloses a lactobacillus rhamnosus R7970 as well as a product and application thereof. The lactobacillus rhamnosus R7970 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.22244. The lactobacillus rhamnosus R7970 is a lactobacillus rhamnosus R7970. Compared with an original strain M9, the lactobacillus rhamnosus R7970 provided by the invention has the advantages that the bacterial colony is obviously increased, the surface is smooth, the edge is neat, the capsular can be obviously observed after the capsular staining, the surface roughness is obviously increased, the smooth degree is obviously reduced, the boundary between the cell wall and the outer capsular is clear, and more capsular polysaccharide is generated; the capsular polysaccharide has higher resistance to the environment, the survival rate of the capsular polysaccharide in intestinal tracts is improved, and the capsular polysaccharide has immunocompetence; in addition, the viscosity of the fermentation liquor can be remarkably increased, and the method is extremely suitable for preparing high-viscosity fermentation products.

The invention provides a method for brewing white fungus-jujube wine. The method comprises the following steps: picking up, cleaning and soaking white fungus, adding water for pulping, grinding via a colloid mill, burdening, inoculating brewer yeast, fermenting, filtering, post fermenting, ageing, clarifying, filtering, conditioning, filling, sterilizing and obtaining a finished product. The health care wine combines the dual nutrition and functions of white fungus and jujube, contains sufficient soluble polysaccharide, vitamins, minerals and the like, prevents calcium loss, prevents osteoporosis and anemia and the like, and also has the functions of softening blood vessels, lowering blood pressure, improving human immunity, reinforcing disease resistance and the like, the health care wine is sufficient in nutrition, comprehensive in healthcare function and is sour, sweet and tasty, thereby being suitable for people of all ages, and meanwhile the brewing method is simple and easy to operate.

The invention provides postoperative rehabilitation formula powder. The postoperative rehabilitation formula powder is prepared from the flowing raw materials in percentage by mass: 30%-40% of fructose syrup dry powder, 10%-20% of soybean hydrolyzed peptide, 10%-20% of concentrated whey protein powder F700, 5%-15% of medium chain triglyceride, 5%-15% of vegetable fat powder, 1%-10% of marine fisholigopeptide, 3%-8% of resistance dextrin, 1%-3% of edible flavor, 0.02%-0.3% of food colouring, 3%-5% of nutrient supplements and 0.03%-0.07% of citric acid. Through the formula design, the postoperative rehabilitation formula powder has reasonable composition setting and balanced nutritional ratio, is beneficial to digestion and absorption of postoperative crowd, improves immunity, accelerates wound healing, maintains nitrogen balance in the body, shortens hospitalization time and accelerates recovery.

The invention discloses a monopterus albus estrogen receptor alpha gene, an encoding protein and an enzyme-linked immunosorbent assay method. The enzyme-linked immunosorbent assay method comprises the following steps: obtaining an ER alpha sequence in monopterus albus gonad by adopting an RT-PCR method, selecting cDNA sequence corresponding to an amino acid sequence with stronger antigenicity as a template to clone the sequence of which length is 945bp, cloning the sequence into a pMAL-c2x expression vector, obtaining purer recombinant protein with immunocompetence through IPTG induction and Amylose-sepharose columella purification, and obtaining ER alpha expression protein capable of being used as immunogen by adopting FactorXa protein enzyme digestion after further purification. A mice can obtain antiserums with higher titer after being immunized, and an immunohistochemical research is preliminarily conducted, shows that the antiserums can be peculiarly bond with ER alpha protein in the monopterus albus gonad, shows positive reaction on oocyte membrane, and lays a foundation for further researching functions of estrogen, ER alpha of the estrogen and the like in gender development of the monopterus albus. According to the invention, an ELISA assay kit is further prepared by taking the antibody as the basis.

The invention discloses a broiler chicken feed additive. The feed additive is prepared by mixing powder of Qianzhong wild buckwheat root tuber growing in an area with the altitude of 800 meters or above and a Chinese herbal medicine mixture. The mass ratio of the Qianzhong wild buckwheat root tuber powder to the Chinese herbal medicine mixture is (4-5): 6, and the Chinese herbal medicine mixture comprises the following components in parts by weight: 2 parts of polygonum aviculare, 4 parts of sanguisorba officinalis and 2 parts of lindera aggregata. The broiler chicken feed additive disclosed by the invention has the comprehensive effects of clearing away heat and toxic materials, relieving cough and reducing phlegm, replenishing qi to invigorate the spleen, resisting bacteria and killing insects, arresting convulsion and promoting growth and improving immunity, and can well promote growth of broiler chickens, increase the weight gain of the broiler chickens, reduce the feed conversion ratio, improve the production performance and enhance the organism immunity of the broiler chickens.