Preparation method and application of a water-soluble chitosan/γ-polyglutamic acid nanocomposite loaded with tanshinone Ⅱa

A technology of water-soluble chitosan and nano-composite, which is applied in the field of pharmaceutical and pharmacy research, can solve the problems of large administration volume, large amount of auxiliary materials, low drug loading amount, etc., and achieves improved curative effect, simple preparation method, and improved in vivo improvement. The effect of absorption properties

Active Publication Date: 2021-04-16
NORTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these preparations still generally have the problem of low drug loading, which leads to a large volume of administration and a large amount of excipients introduced into the body, which has the risk of causing potential toxicity

Method used

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  • Preparation method and application of a water-soluble chitosan/γ-polyglutamic acid nanocomposite loaded with tanshinone Ⅱa
  • Preparation method and application of a water-soluble chitosan/γ-polyglutamic acid nanocomposite loaded with tanshinone Ⅱa
  • Preparation method and application of a water-soluble chitosan/γ-polyglutamic acid nanocomposite loaded with tanshinone Ⅱa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Precisely weigh γ-PGA dissolved in ultrapure water to make 0.125 mg / ml γ-PGA aqueous solution, weigh 60mg CS and dissolve it in 10ml ultrapure water, weigh 12mg Tanshinone II A Dissolve in 12ml of absolute ethanol, Tanshinone Ⅱ A Add 12ml of γ-PGA solution with a concentration of 0.125 mg / ml to the ethanol solution, place it on a magnetic stirrer and stir for 10min at a speed of 500rpm / min, then slowly add the mixed solution dropwise to the above CS solution, and continue magnetically stirring for 1h , The speed is 1000rpm / min. After the reaction is completed, the suspension is rotary evaporated until the organic solvent is evaporated to obtain CS-γ-PGA-Tanshinone II A clathrate solution. Jiang CS-γ-PGA-Tanshinone Ⅱ A CS-γ-PGA-tanshinone Ⅱ obtained by freeze-drying the clathrate solution A Clathrate powder.

Embodiment 2

[0033] Precisely weigh γ-PGA dissolved in ultrapure water to make 0.25mg / ml γ-PGA aqueous solution, weigh 40mg CS and dissolve in 10ml ultrapure water, weigh 5mg Tanshinone II A Dissolve in 12ml of absolute ethanol, Tanshinone Ⅱ AAdd 8 ml of γ-PGA solution with a concentration of 0.25 mg / ml to the ethanol solution, place it on a magnetic stirrer and stir for 7 minutes at a speed of 300 rpm / min, then slowly add the mixed solution dropwise to the above CS solution, and continue magnetic stirring for 1.5 h, the speed is 2000rpm / min. After the reaction is completed, the suspension is rotary evaporated until the organic solvent is evaporated to obtain CS-γ-PGA-Tanshinone II A clathrate solution. Jiang CS-γ-PGA-Tanshinone Ⅱ A CS-γ-PGA-tanshinone Ⅱ obtained by freeze-drying the clathrate solution A Clathrate powder.

Embodiment 3

[0035] Precisely weigh γ-PGA dissolved in ultrapure water to make 0.4mg / ml γ-PGA aqueous solution, weigh 80mg CS and dissolve in 10ml ultrapure water, weigh 16mg Tanshinone II A Dissolve in 10ml of absolute ethanol, Tanshinone Ⅱ A Add 10ml of γ-PGA solution with a concentration of 1.6 mg / ml to the ethanol solution, place it on a magnetic stirrer and stir for 20min at a speed of 400rpm / min, then slowly add the mixed solution dropwise to the above CS solution, and continue magnetic stirring for 1h , The speed is 3000rpm / min. After the reaction is completed, the suspension is rotary evaporated until the organic solvent is evaporated to obtain CS-γ-PGA-Tanshinone II A clathrate solution. Jiang CS-γ-PGA-Tanshinone Ⅱ A CS-γ-PGA-tanshinone Ⅱ obtained by freeze-drying the clathrate solution A Clathrate powder.

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Abstract

A preparation method and application of a water-soluble chitosan / γ-polyglutamic acid nanocomposite loaded with tanshinone ⅡA, in proportion, respectively adding γ-polyglutamic acid and water-soluble chitosan to distilled water to dissolve, Dissolve tanshinone ⅡA in absolute ethanol, then add the ethanol solution of tanshinone ⅡA into the aqueous solution of γ-polyglutamic acid and mix well, add the mixed solution dropwise into the aqueous solution of chitosan, stir while adding, continue to magnetically Stir for 1 hour at 1000 revolutions per minute. Then the organic solvent was removed by rotary evaporation, frozen at -20°C, and then freeze-dried to obtain a solid powder. The preparation method of the present invention is simple and easy, and the prepared water-soluble chitosan / γ-polyglutamic acid nanocomposite of tanshinone IIA can greatly increase the drug release performance of tanshinone IIA in vivo and in vitro, thereby increasing the bioavailability of tanshinone IIA .

Description

technical field [0001] The invention belongs to the field of pharmaceutical pharmacy research, and in particular relates to a preparation method and application of a water-soluble chitosan / γ-polyglutamic acid nanocomposite loaded with tanshinone IIA. Background technique [0002] According to the statistics of the World Health Organization, the morbidity and mortality of cardiovascular and cerebrovascular diseases have leapt to the top of all kinds of diseases. According to statistics from the Ministry of Health, the morbidity and mortality of cardiovascular and cerebrovascular diseases in my country rank first among various diseases, and the prevalence and mortality of cardiovascular diseases are still on the rise. It is estimated that the number of patients with cardiovascular diseases in my country is 290 million. So far, there is no effective drug for treatment. The main reason is that the cells that make up the blood brain barrier (BBB) ​​are extremely tightly connected...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/19A61K47/36A61K47/34A61K31/58A61P9/00A61P9/10
CPCA61K9/19A61K31/58A61K47/34A61K47/36A61P9/00A61P9/10
Inventor 于洁吴妮郑晓晖张亚军
Owner NORTHWEST UNIV
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