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Preparation method of rice enzymolysis selenium polypeptides with immune activity

A technology of immune activity and rice, which is applied in the field of preparation of rice enzymatically hydrolyzed selenium polypeptides to achieve high utilization value and avoid waste

Active Publication Date: 2014-01-08
湖南瑶珍粮油有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are not many research data related to the biological activity of rice protein, but the existing analysis studies are sufficient to confirm that rice protein hydrolyzate has the characteristics of anti-oxidation, anti-virus and immune regulation.

Method used

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  • Preparation method of rice enzymolysis selenium polypeptides with immune activity
  • Preparation method of rice enzymolysis selenium polypeptides with immune activity
  • Preparation method of rice enzymolysis selenium polypeptides with immune activity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Raw material pretreatment: grind the same batch of selenium-enriched broken rice with a pulverizer, and then pass through a 100-mesh sieve.

[0028] (2) Degreasing: use n-hexane, the ratio of solid to liquid is 1:5, degrease twice on a magnetic stirrer, 3 hours each time, centrifuge, and air-dry the precipitate overnight in a fume hood.

[0029] (3) Alkaline extraction: Alkaline extraction of defatted selenium-enriched rice flour twice with NaOH concentration of 0.07mol / L, solid-liquid ratio of 1:15, temperature of 40°C, time of 3h, centrifugation, and supernatant combined.

[0030] (4) Acid precipitation: put the obtained protein hydrolyzate on a magnetic stirrer, adjust to the isoelectric point (4.5) with dilute hydrochloric acid solution while stirring, wait for the precipitation to separate out, centrifuge again, and wash with water twice.

[0031] (5) Freeze-drying: put the protein obtained above into a petri dish and put it in the refrigerator, pre-freeze over...

Embodiment 2

[0035] (1) Raw material pretreatment: grind the same batch of selenium-enriched broken rice with a pulverizer, and then pass through a 100-mesh sieve.

[0036] (2) Degreasing: use n-hexane, the ratio of solid to liquid is 1:6, degrease twice on a magnetic stirrer, 4 hours each time, centrifuge, and air-dry the precipitate overnight in a fume hood.

[0037] (3) Alkaline extraction: Alkaline extraction of defatted selenium-enriched rice flour twice with NaOH concentration of 0.07mol / L, solid-liquid ratio of 1:10, temperature of 40°C, time of 4h, centrifugation, and supernatant combined.

[0038] (4) Acid precipitation: put the obtained protein hydrolyzate on a magnetic stirrer, adjust to the isoelectric point (4.5) with dilute hydrochloric acid solution while stirring, wait for the precipitation to separate out, centrifuge again, and wash with water twice.

[0039] (5) Freeze-drying: put the protein obtained above into a petri dish and put it in the refrigerator, pre-freeze over...

Embodiment 3

[0043] (1) Raw material pretreatment: grind the same batch of selenium-enriched broken rice with a pulverizer, and then pass through a 100-mesh sieve.

[0044] (2) Degreasing: use n-hexane, the ratio of solid to liquid is 1:5, degrease twice on a magnetic stirrer, 3 hours each time, centrifuge, and air-dry the precipitate overnight in a fume hood.

[0045] (3) Alkaline extraction: Alkaline extraction of defatted selenium-enriched rice flour twice with NaOH concentration of 0.07mol / L, solid-liquid ratio of 1:20, temperature of 40°C, time of 4h, centrifugation, and supernatant combined.

[0046] (4) Acid precipitation: put the obtained protein hydrolyzate on a magnetic stirrer, adjust to the isoelectric point (4.5) with dilute hydrochloric acid solution while stirring, wait for the precipitation to separate out, centrifuge again, and wash with water twice.

[0047] (5) Freeze-drying: put the above-mentioned obtained protein into a petri dish and put it in the refrigerator, pre-f...

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Abstract

The invention relates to the processing field of a selenium-rich rice by-product, in particular to a preparation method of rice enzymolysis selenium polypeptides with immune activity. According to the method, selenium-rich rice processing by-product broken rice is used to prepare degreased selenium-rich rice powder by grinding, sieving and degreasing, a supernatant is collected after alkaline extraction and centrifugation by using a sodium hydroxide solution, a dilute hydrochloric acid solution is used for acid precipitation, and after centrifugation, a precipitate is taken for washing and then is frozen-dried. The obtained rice proteins, after enzymolysis by using trypsase, are frozen-dried to obtain the rice enzymolysis polypeptides with the selenium content of 0.3-0.4mg / kg. In a cell immune function experiment, the rice enzymolysis selenium polypeptides have good capacity for promotion of cell proliferation and neutral red phagocytosis of macrophage. The rice enzymolysis selenium polypeptides are natural in nutrition, rich in trace element selenium, and suitable to use as auxiliary food materials, nutrient supplements or health food for enhancing immune functions of bodies, and the preparation method is simple and reliable.

Description

1. Technical field [0001] The invention relates to the field of processing selenium-enriched rice by-products, in particular to a method for preparing a rice enzymatically hydrolyzed selenium polypeptide with immune activity, which belongs to the field of functional food processing. 2. Background technology [0002] Selenium is an essential micronutrient element for humans and animals, and has become the focus of attention at home and abroad. In recent years, some scholars have found that selenium has a certain inhibitory effect on tumor growth, and selenium deficiency is closely related to the occurrence of certain diseases. Studies have shown that selenium is also the active center of some selenium-containing enzymes involved in cellular anti-oxidation, such as glutathione peroxidase and oxidoreductase. Most of the selenium in rice exists in the form of protein selenium binding. Some scholars have found that selenium in selenium-enriched rice mainly exists in the form of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 方勇胡秋辉罗佩竹徐卫东高德泉陈曦陈悦徐迅李友良邱伟芬杨文建马宁鞠兴荣
Owner 湖南瑶珍粮油有限公司
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