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Preparation method and application of agaricus bisporus polysaccharide with immunocompetence

A Agaricus bisporus, immune activity technology, applied in the field of Agaricus bisporus polysaccharide and its preparation, can solve the problems of the source of activity, the relationship between activity and structure, but the progress is slow

Inactive Publication Date: 2014-02-19
QINGDAO AGRI UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0003] The health benefits of Agaricus bisporus have been confirmed by a long history of consumption, but the relationship between its source of activity, activity and structure is progressing slowly

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Preparation of Agaricus bisporus polysaccharide:

[0019] (a) Prepare 1000g of Agaricus bisporus, dry and crush at 45°C, add 3000g of distilled water, extract in a water bath at 75°C, filter, and concentrate in vacuo;

[0020] (b) Precipitate with 80% ethanol, centrifuge (4500r, 15min) and discard the supernatant;

[0021] (e) Segva deproteinization 6 times;

[0022] (f) select ion-exchange filler (D201, DEAE-52, CM52) to carry out ion-exchange column chromatography to obtain ABPI;

[0023] (g) Select gel column packing (Sephadex G-200, Bio-Gel-p-4, sepharose 4B, sephacryl S100, sephacryl S300) to perform gel column chromatography to obtain 1000 mg of polysaccharide ABPla polysaccharide structure analysis method:

[0024] (1) Determination of the molecular weight range of Agaricus bisporus polysaccharide ABPla by high pressure gel exclusion chromatography (HPGPC);

[0025] (2) The glycosidic bond structure is analyzed by periodate oxidation method;

[0026] (3) ...

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Abstract

An agaricus bisporus polysaccharide with immunocompetence is a heteropolysaccharide consists of ribose, rhamnose, arabinose, xylose, mannose, glucose, and galactose at a molar ratio of 2.08:4.61:2.45:22.25:36.45:89.22:1.55. The agaricus bisporus polysaccharide is prepared by extracting agaricus bisporus in a water bath; precipitating the extract with ethanol; deproteinizing the precipitate to obtain coarse polysaccharide; processing the coarse polysaccharide by ion exchange column chromatography with an anion exchange packing; eluting with distilled water; carrying out freeze drying; carrying out gel chromatography; eluting with distilled water; carrying out freeze drying to obtain the final product. The agaricus bisporus polysaccharide has immunological enhancement effect, and can serve as adjuvant drug for treating immunodeficiency diseases, such as cancer, or serve as functional health products and foods for enhancing immunity.

Description

technical field [0001] The present invention relates to an immune active polysaccharide, in particular to a type of immune active Agaricus bisporus polysaccharide and its preparation method and application. technical background [0002] Agaricus bisporus is flat in nature and sweet in taste, can invigorate the spleen and qi, and can enhance the body's ability to resist diseases. It has a long history of consumption. It is the edible fungus with the most extensive cultivation, the highest output and the largest consumption in the world at present. It is also the product that earns the most foreign exchange from the export of edible fungi in my country. According to statistics from the China Edible Fungi Association, the output of Agaricus bisporus in my country reached 20 million tons in 2009, mainly exported as processed products such as canned mushrooms, dried mushroom slices, and salted mushrooms. A polysaccharide (PS-K) can be extracted from mushrooms, which has anti-vi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L1/09A61P35/00A61P37/04A23L29/30
Inventor 不公告发明人
Owner QINGDAO AGRI UNIV
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