Feed rich in probiotics and active peptide and application of feed

A technology of active peptides and probiotics, applied in the application, animal feed, animal feed and other directions, can solve the problems of antibiotic accumulation, harm to human health, accumulation, etc., to achieve the effect of simple operation, short enzyme production cycle, and convenient large-scale production

Pending Publication Date: 2017-08-04
QIQIHAR UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in order to make the farmed poultry and livestock grow healthily and improve the disease resistance of the farmed livestock, certain antibiotics will be added to the solid-state fermented feed. It will cause antibiotic resistan

Method used

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  • Feed rich in probiotics and active peptide and application of feed
  • Feed rich in probiotics and active peptide and application of feed
  • Feed rich in probiotics and active peptide and application of feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The Bacillus licheniformis strain with the preservation number CGMCC No. 13109 and the Lactobacillus strain with the preservation number CGMCC No. 13110 were inoculated on the slant medium, and cultured in an incubator at 37° C. for 24 hours. Activate two generations. The formula of the Bacillus licheniformis slant medium is as follows: 1% peptone, 1% sodium chloride, 0.5% yeast extract and 2.5% agar, and the pH value is 7.0. Lactobacillus medium formula is as follows: 1% casein peptone, 1% beef extract, 0.5% yeast extract, 0.25% glucose, 0.5% sodium acetate, 0.2% diammonium citrate, 0.1% Tween 80, 0.2% dihydrogen phosphate Potassium, 0.2% anhydrous magnesium sulfate, 0.005% manganese sulfate, 2% calcium carbonate, 2.5% agar, the pH value is 6.4.

[0048] Inoculate the activated Bacillus licheniformis and Lactobacillus into the liquid seed medium according to the inoculation amount of 5%, and cultivate them on a shaking table with a rotation speed of 180r / min at 37°C f...

Embodiment 2

[0061] Mix corn gluten powder and bran in a mass ratio of 7:3 to form a mixture, the mass volume ratio of the mixture to water is 1g:1.3mL, and insert the Bacillus licheniformis and Lactobacillus prepared in Example 1 Seed solution, the inoculum of Bacillus licheniformis is 5×10 8 CFU / g feed liquid, the inoculation amount of Lactobacillus is 1×10 9 CFU / g feed solution, natural pH, temperature 37°C, first add Bacillus licheniformis for fermentation, then add Lactobacillus after 48h interval, and co-ferment for 48h to obtain the fermentation product.

[0062] (1) The content of soluble peptides in the fermentation product was detected by the Folin-phenol method.

[0063] (2) The oxidation resistance adopts pyrogallol autoxidation method.

[0064] Pyrogallol is stable in acidic environment, but autoxidation reaction occurs in weak alkaline environment,

[0065] Glycinol only accepts one electron to generate superoxide anion radical (O 2 +O 2 - ),and

[0066] In its autoxid...

Embodiment 3

[0083] Mix corn gluten powder and bran at a mass ratio of 3:2 to form a mixture, the mass volume ratio of the mixture to water is 1g:1.5mL, and insert the Bacillus licheniformis and Lactobacillus prepared in Example 1 Seed solution, the inoculum of Bacillus licheniformis was 1×10 9 The inoculation amount of CFU / g feed liquid and lactic acid bacteria is 1×10 10 CFU / g feed liquid, natural pH, temperature 35°C, first add Bacillus licheniformis, then add Lactobacillus after an interval of 36 hours, and co-ferment for 64 hours to obtain a fermentation product.

[0084] (1) The content of soluble peptides in the fermentation product was detected by the Folin-phenol method. The test result showed that the soluble peptide content was 94.02mg / g.

[0085] (2) According to the method described in Example 2, the oxidation resistance of the fermentation product was detected. The test results showed that the antioxidant activity of the fermentation product was 95U / mL.

[0086] (3) Detect ...

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Abstract

The invention provides a feed rich in probiotics and active peptide and an application of the feed and belongs to the field of feed production. The feed rich in the probiotics and the active peptide is fermentation product of a mixed solution of a mixed material formed by corn gluten meal and bran and water under the common fermentation action of bacillus licheniformis and lactobacillus, wherein the mass concentration of the active peptide in the feed is 94-100mg/g; the cell concentration of the probiotics in the feed is (0.5-2.0)*10<9>CFU/g; and bacillus licheniformis is firstly inoculated into a feed liquid for separate fermentation before fermentation and lactobacillus is inoculated into the feed liquid for common fermentation. According to the feed, the palatability of the feed can be significantly improved and the food intake of animals is improved, so that the production performance of the animals is improved. Meanwhile, the immune performance of the animals can be improved by the feed.

Description

technical field [0001] The invention belongs to the technical field of feed, and in particular relates to a feed rich in probiotics and active peptides and an application thereof. Background technique [0002] With the trend of environmental protection, energy saving and green production becoming more and more prominent, the use of industrial and agricultural by-products for microbial fermentation to produce protein feed is more in line with the requirements of modern people. There are many feed materials currently prepared, such as beer and liquor distillers grains, residues left over from soy sauce production, starch residues, apple residues, sugar beet residues, and cottonseed cakes, paulownia cakes, sesame cakes, etc. from oil processing; or they can also be used Slaughtering plants waste leftovers, livestock and poultry manure, and waste generated during deep processing of seafood. [0003] In the prior art, both enzymatic method and microbial fermentation can be effec...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K20/147A23K10/37
CPCY02P60/87
Inventor 刘晓兰王燕江成英郑喜群任健
Owner QIQIHAR UNIVERSITY
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