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Method for preparing nano-silver composite particles from emblica polysaccharide

A technology of composite particles and emblical polysaccharides, which is applied in the field of nanomaterials, can solve the problems of reducing the surface activity of nanoparticles, environmental pollution, and affecting the performance of nanoparticles, so as to achieve the effect of improving the utilization value and green process

Active Publication Date: 2017-11-03
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

These organic and inorganic reducing agents may cause environmental pollution or have biological toxicity
In addition, in the synthesis process, in order to avoid the agglomeration of silver nanoparticles, it is often necessary to add a dispersant to reduce the surface activity of the nanoparticles, which also brings about environmental pollution and affects the performance of the synthesized nanoparticles.

Method used

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  • Method for preparing nano-silver composite particles from emblica polysaccharide
  • Method for preparing nano-silver composite particles from emblica polysaccharide
  • Method for preparing nano-silver composite particles from emblica polysaccharide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Preparation of emblical polysaccharide and nano silver

[0031] The first step: preparation of emblical polysaccharide: samples of emblica (powdered ganache) were collected from Lantian Village, Zishan Town, Hui'an County, Quanzhou City, Fujian Province. The preparation of emblica polysaccharide (Emblica Polysaccharide, EPS) first selects fresh and disease-free emblica fruit, washes, air-dries, removes the core, freeze-dries, passes through a 40-mesh sieve to obtain emblica powder, extracts in hot water for 5 hours and centrifuges Take the supernatant to obtain the emblica extract, concentrate by rotary evaporation, dehydrate with ethanol, and dry repeatedly with ether to obtain the crude polysaccharide of emblica, and finally obtain the crude polysaccharide of emblica through freeze-thaw classification, Sevage method for protein removal, dialysis, rotary evaporation, concentration and freeze-drying to obtain emblica polysaccharides.

[0032] The second st...

Embodiment 2

[0033] The carrying out of embodiment 2 antibacterial test

[0034] The first step: plate method detection antibacterial test: this test selects AgNO 3 The three factors of polysaccharide volume ratio, reaction time and NaCl volume were used for single-factor antibacterial test, and each factor took five levels respectively. Staphylococcus aureus was used as the test strain, and the plate method was used to carry out antibacterial test to detect the antibacterial ability of AgNPs. The AgNPs powders prepared under different conditions were prepared into 10 μg / mL AgNPs solutions with double distilled water, and ultrasonicated for 30 min to disperse the solutions evenly before use. In the bacteriostasis test, the activated bacterial liquid was added to the LB solid medium at a ratio of 1%. After the medium plate was solidified, a 6mm circular filter paper was placed in the center of the LB medium, and the filter paper Spot 15 μL of prepared different AgNPs solutions on the top ...

Embodiment 3

[0038] Embodiment 3 response surface test

[0039] Step 1: According to the single factor test results and the Box-Benhnken center combination test design principle, continue to optimize the conditions for preparing AgNPs from emblical polysaccharide within the test range. Wherein with the size (Y) of the inhibition zone as the response value, the best three-factor combination obtained according to Example 2 is respectively AgNO 3 The volume ratio to polysaccharide (A), NaCl volume (B), and time (C) are three factors, and the response surface optimization experiment is carried out with 3 factors and 3 levels, and each experiment is repeated three times. There are 17 test points in the response surface analysis test, including 5 zero points and 12 analysis point tests, which are used to estimate the test error. The test factors and levels are shown in Table 1.

[0040] Table 1 Response surface analysis factors and levels

[0041]

[0042]

[0043] The second step: data...

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Abstract

The invention provides a method for preparing nano-silver composite particles from emblica polysaccharide and belongs to the technical field of nanometer materials. The method comprises the steps of preparation of emblica polysaccharide and preparation of nano-silver. According to the prepared composite particles, the diameter, measured through bacteria inhibition experiments, of inhibition zones is 27.9+ / -0.01 mm; a theoretical value and an experimental value are basically equal. This shows that the process for preparing AgNPs through a response surface optimization method is green and reliable.

Description

technical field [0001] The invention relates to the technical field of nanomaterials, in particular to a method for preparing nano-silver composite particles from emblica polysaccharide. Background technique [0002] Due to the small particle size, large specific surface area, high surface energy, and large proportion of surface atoms, nanomaterials exhibit three major effects: surface effect, small size effect, and macroscopic quantum tunneling effect, so the particles have good antibacterial properties. active. Therefore, the study of silver nanoparticles has attracted more and more attention and favor of scientific researchers, especially the silver nanoparticles in them have been proved to have obvious inhibitory effect on bacteria, viruses and other prokaryotes. Chemical synthesis is the most commonly used method for synthesizing nano silver. This method is widely used because of its simple conditions, low cost, large yield, controllable nanoparticles and easy operatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24B82Y40/00
CPCB22F9/24B22F2999/00B82Y40/00B22F2202/11
Inventor 李永裕吴少华廖正萍刘靖媛王芳李亮王文婷
Owner FUJIAN AGRI & FORESTRY UNIV
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