38 results about "Bisulfite sequencing" patented technology

Systems and methods that analyze blood-based cancer diagnostic tests using multiple classes of molecules are described. The system uses machine learning (ML) to analyze multiple analytes, for example cell-free DNA, cell-free microRNA, and circulating proteins, from a biological sample. The system can use multiple assays, e.g., whole-genome sequencing, whole-genome bisulfite sequencing or EM-seq, small-RNA sequencing, and quantitative immunoassay. This can increase the sensitivity and specificity of diagnostics by exploiting independent information between signals. During operation, the system receives a biological sample, and separates a plurality of molecule classes from the sample. For a plurality of assays, the system identifies feature sets to input to a machine learning model. The system performs an assay on each molecule class and forms a feature vector from the measured values. The system inputs the feature vector into the machine learning model and obtains an output classification of whether the sample has a specified property.

The invention relates to a kit for morbidity-related tumor suppressor gene epigenetic mutation detection of gastrointestinal neoplasms and application thereof. The kit mainly comprises 25 pairs of gastrointestinal neoplasm morbidity-related tumor suppressor gene promotor region CpG-island amplification and sequencing primers of 9 kinds and a polymerase chain reaction (PCR) amplification reagent. Through a PCR amplification product obtained through the kit, a gene CpG-island methylation state can be analyzed through the methods of methylation specific PCR (MSP), combined bisulphite-restrictionanalysis (COBRA) or bisulfite sequencing PCR (BSP) and the like. The kit has the efficient effect of detecting CpG-island methylation and is used for the morbidity-related tumor suppressor gene epigenetic mutation detection of the gastrointestinal neoplasms.

Provided is a high throughput methylation detection method, particularly a combined sequence capture and bisulfite sequencing method. The method accurately and effectively analyzes the methylation status of the target area in several samples simultaneously, lowers the difficulty of probe design, enhances operation and application feasibility, and enables high throughput methylation detection of high accuracy on interested target sequences and areas in a complete genome. The method is targeted and conserves energy and time.

The invention relates to a cancer screening method which comprises the following steps that: (1) providing a tested body; (2) testing the CpG sequence methylation state of at least one target gene in genome DNA of the tested body, and the target gene comprises PTPRR, ZNF582, PDE8B and DBC1; (3) judging whether the tested body has cancer or lesions before cancer according to that whether the methylation state of the at least one target gene exists; and the methylation state detection method is methylation-specific PCR, MSP, quantitative methylation-specific PCR, QMSP, bisulfite sequencing (BS), microarrays, massspectrometer, denaturing high-performance liquidchromatography (DHPLC) and the like.

A cancer screening method comprises the following steps: (1) a tested body is provided; (2) the methylation state of a CpG sequence of at least one target gene of genome DNA of the tested body is detected, wherein the target gene comprises SOX1, PAX1, LMX1A, NKX6-1, WT1 and ONECUT1; (3), whether the tested body has a cancer or a precancerous lesion is judged according to the methylation state of the target gene. A methylation state detection method adopts MSP (methylation-specific PCR), QMSP (quantitative methylation-specific PCR), BS (bisulfite sequencing), microarrays, mass spectrometer, DHPLC (denaturing high-performance liquid chromatography) and the like.

The invention relates to a library construction method, a sequencing method and a methylation detection method for sequencing 5-methylcytosine (5-methylcytosine, 5mC) modified bisulfite on RNA molecules. By designing and using a three-base random hexamer primer containing only ACT, the efficiency of reverse transcription and PCR amplification of RNA fragments treated with bisulfite and the detection efficiency of 5mC sites are significantly improved, which is beneficial to the detection of 5mC sites high-throughput sequencing.

The invention discloses a molecular marker of DNA methylation of large yellow croaker, a screening method of the molecular marker and the application of the molecular marker in breeding of large yellow croaker; the molecular marker of DNA methylation of large yellow croaker includes molecular marker fgf11, molecular marker fgfrs2, molecular marker egflp7 and molecular marker pdgfrα. The method for screening molecular markers of large yellow croaker DNA methylation provided by the present invention, on the basis of the genetic information of the large yellow croaker genome, combines high-throughput RNA-Seq, genome resequencing and bisulfite sequencing technologies to carry out large-scale Study on the epigenetic differential characteristics of populations with significant differences in growth traits of yellow croaker, and screen the differential methylation characteristics of genes on the whole genome level; the molecular markers of DNA methylation of large yellow croaker provided by the present invention can be used in large yellow croaker breeding The application in assisted breeding research can effectively improve the breeding rate of fine varieties of large yellow croaker.