Simplified method and kit for constructing library for apparent bisulfite sequencing

Abstract

According to the present invention, the simplified apparent bisulfite sequencing library construction method and library construction kit are applicable to low-quality samples such as paraffin-embedded samples.

Description

technical field [0001] The invention belongs to the field of gene detection, and in particular relates to a method for constructing a simplified apparent bisulfite sequencing library applicable to low-quality samples such as paraffin-embedded samples. Background technique [0002] DNA methylation is the most common epigenetic modification of genomic DNA. A large number of studies have shown that DNA methylation plays a vital role in maintaining normal cell function, transmitting genomic imprinting, embryonic development, and human tumorigenesis. effect. With the birth of high-throughput sequencing, research on DNA methylation has become more and more in-depth. As the gold standard for DNA methylation detection, whole-genome bisulfite sequencing WGBS or BS-Seq (Whole genome bisulfate sequencing) [1] , which can analyze the DNA methylation of the whole genome, and has the detection sensitivity at the single base level. However, it is precisely because BS-Seq can detect the m...

AI Technical Summary

Problems solved by technology

[0015] (1) For paraffin-embedded samples or other low-quality samples, the data waste is serious and the data utilization rate is low;

[0016] (2) RRBS library construction generally requires the amount of gDNA in the initial sample to be 2-5 μg, which makes it difficult for precious (generally speaking, a small amount) clinical samples to meet the requirements for the initial amount of library construction

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