Molecular Markers of DNA Methylation in Large Yellow Croaker and Its Application in Breeding

A technology of molecular markers and large yellow croaker, which is applied in the direction of recombinant DNA technology, DNA/RNA fragments, climate change adaptation, etc., can solve the problems of insufficient molecular regulation mechanism and genetic basis related to growth traits of large yellow croaker, and achieve the promotion of large yellow croaker species. quality, improve quality, and improve the effect of breeding rate

Active Publication Date: 2022-05-10
NINGDE NORMAL UNIV
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  • Abstract
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Problems solved by technology

However, the research on the molecular regulation mechanism and genetic basis of the growth traits of large yellow croaker is not enough, and the transcriptome and epigenetic regulation mechanism at the overall level of the growth axis have not been reported yet.

Method used

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  • Molecular Markers of DNA Methylation in Large Yellow Croaker and Its Application in Breeding
  • Molecular Markers of DNA Methylation in Large Yellow Croaker and Its Application in Breeding
  • Molecular Markers of DNA Methylation in Large Yellow Croaker and Its Application in Breeding

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Embodiment 1

[0063] The method for screening the molecular markers of large yellow croaker DNA methylation of the present invention, comprises the following steps:

[0064] Step 1. The experimental materials come from the "Fufa No. 1" large yellow croaker strain of Ningde Fufa Aquatic Products Co., Ltd., and the large yellow croaker was cultivated in accordance with the technical specifications for large yellow croaker seed breeding of Ningde Fufa Aquatic Products Co., Ltd.; A total of 314 F4 seedlings of the new line about 13 months old were randomly selected from the same net cage as the model. Biological measurements and marks were made for each fish, and the muscles the size of rice grains were cut and stored in absolute ethanol. The group of 40 high-value individuals with the largest body weight was marked as the very large individual group (L group), and the group of 40 low-value individuals with the smallest body weight was marked as the very small individual group (S group).

[006...

Embodiment 2

[0158] The application of molecular markers of large yellow croaker DNA methylation in large yellow croaker breeding includes the following steps:

[0159] Step a, extract DNA from the muscle tissue of large yellow croaker (Shanghai Jierui company DNA extraction kit), after sulfite conversion, use (SEQ ID No.5-SEQ ID No.12) shown in table 1 Primers for PCR amplification;

[0160] Step b, detect PCR amplification product, with reference to the molecular marker fgf11 shown in table 1 (SEQ ID No.1-SEQ ID No.4), molecular marker fgfrs2, molecular marker egflp7 and molecular marker pdgfrα, select fgf11 gene, fgfrs2 Individuals with low average methylation frequency of gene, egflp7 gene and pdgfrα gene were used as breeding parents.

[0161] In summary, the method for screening molecular markers of large yellow croaker DNA methylation provided by the present invention uses WGBS to detect the DNA methylation characteristics of large yellow croaker, and screens the methylation profiles...

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Abstract

The invention discloses a molecular marker of DNA methylation of large yellow croaker, a screening method of the molecular marker and the application of the molecular marker in breeding of large yellow croaker; the molecular marker of DNA methylation of large yellow croaker includes molecular marker fgf11, molecular marker fgfrs2, molecular marker egflp7 and molecular marker pdgfrα. The method for screening molecular markers of large yellow croaker DNA methylation provided by the present invention, on the basis of the genetic information of the large yellow croaker genome, combines high-throughput RNA-Seq, genome resequencing and bisulfite sequencing technologies to carry out large-scale Study on the epigenetic differential characteristics of populations with significant differences in growth traits of yellow croaker, and screen the differential methylation characteristics of genes on the whole genome level; the molecular markers of DNA methylation of large yellow croaker provided by the present invention can be used in large yellow croaker breeding The application in assisted breeding research can effectively improve the breeding rate of fine varieties of large yellow croaker.

Description

technical field [0001] The invention belongs to the technical field of molecular markers, in particular to a molecular marker for DNA methylation of large yellow croaker and its application in breeding. Background technique [0002] Large yellow croaker (Larimichthyscrocea) is a unique seawater economically farmed fish in my country. Its aquaculture and nursery production ranks first in the country's seawater fish and occupies an important position in my country's seawater aquaculture industry. At present, the large yellow croaker industry has entered the stage of transformation and upgrading, and the industry has increasingly strong expectations for excellent varieties (strains) with fast growth, strong stress resistance and high quality. Therefore, large yellow croaker breeding has become an important link in the development of large yellow croaker industry. Molecular breeding technology is a modern biotechnology breeding method newly developed to overcome the problems of...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/6858C12N15/11
CPCC12Q1/6888C12Q1/6858C12Q2600/154C12Q2600/124C12Q2523/125C12Q2531/113Y02A40/81
Inventor 韩坤煌王艺磊张子平刘伟阮少江黄伟卿李菲艳陈欣欣
Owner NINGDE NORMAL UNIV
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