Method and application of ion beam mutagenesis to obtain Dendrobium candidum with high polysaccharide and high yield

Abstract

The invention discloses a method for obtaining high-polysaccharide and high-yield dendrobium officinale kimura ex migo through ion beam mutagenesis and application. The method comprises the followingsteps: selecting full and insect-pest-free seeds and disinfecting; carrying out ion beam mutagenesis treatment on the disinfected dendrobium officinale kimura ex migo seeds; carrying out tissue culture seedling cultivation on the mutagenic seeds; cultivating the obtained tissue culture seedling, selecting plants obtained by seeds which are not subjected to the mutagenesis treatment as a contrast,and screening to obtain high-yield plants with character mutation; independently collecting seeds of the plants with the character mutation and continually carrying out subculture; screening to obtainthe dendrobium officinale kimura ex migo with stable characters, so as to obtain a product. According to the method for obtaining the high-polysaccharide and high-yield dendrobium officinale kimura ex migo through the ion beam mutagenesis, provided by the invention, non-directional mutation can be generated after an ion beam is injected to carry out the mutagenesis; in a lot of mutagenesis screening experiments, screening can be carried out according to a pre-set screening target, so that excellent dendrobium officinale kimura ex migo germplasm with needed characters is screened an obtained;the polysaccharide content of the bred dendrobium officinale kimura ex migo is improved by 13.35 percent on the basis of improving the yield.

Description

technical field [0001] The invention relates to the technical field of dendrobium candidum breeding, in particular to a method and application for obtaining dendrobium candidum with high polysaccharide and high yield through ion beam mutagenesis. Background technique [0002] Dendrobium officinale Kimura et Migo, also known as black knot grass, is a traditional Chinese medicinal material with a long history of food and medicine. As early as in "Shen Nong's Materia Medica", there is a record of "mainly in the injury, eliminates numbness, lowers qi, nourishes the five viscera due to fatigue and thinness, strengthens yin, and thickens the stomach after long-term use". [0003] Dendrobium officinale grows in a special environment, and it is extremely difficult to reproduce itself. Coupled with its high economic value, people have long-term unlimited over-harvesting and irrational development and utilization, resulting in fewer and fewer wild resources. It has been listed as a ra...

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Problems solved by technology

[0005] However, so far, there have been no relevant reports on the ion beam mutagenesis of Dendrobium officinale seeds

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