A kind of anther culture detoxification method of edible lily

A technology for eating lilies and lily flowers, applied in the biological field, can solve the problems of low cultivation efficiency, failure to meet production requirements, and low efficiency of lily anther induction

Inactive Publication Date: 2017-10-03
西诺(北京)花卉种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, the existing edible lily anther culture efficiency is low, far from the production requirements, mainly because the lily anther induction efficiency is too low

Method used

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  • A kind of anther culture detoxification method of edible lily
  • A kind of anther culture detoxification method of edible lily

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] In July 2012, the anthers of edible lily varieties Longya Lilium and Chuan Lily were selected for flower cultivation to induce detoxification. The detailed steps are as follows:

[0060] (1) Preparation of edible lily anther induction medium

[0061] Formulated per liter of distilled water from:

[0062] Macroelements: KNO 3 2600mg / L, NH 4 NO 3 1700mg / L, KH 2 PO 4 460mg / L, MgSO 4 ∙7H 2 O 370mg / L, CaCl 2 ∙2H 2 O 840mg / L;

[0063] Trace elements: MnSO 4 ∙4H 2 O 22.5mg / L, ZnSO 4 ∙7H 2 O 8.5mg / L, H 3 BO 3 6.25mg / L, KI0.825mg / L, CuSO 4 ∙5H 2 O 0.025mg / L, CoCl∙6H 2 O 0.025mg / L, NaMoO 4 ∙2H 2 O 0.25mg / L;

[0064] Iron salt: Na 2 -EDTA∙2H 2 O 37.5mg / L, FeSO 4 ∙7H 2 O 27.5mg / L;

[0065] Organic Ingredients: Inositol 100mg / L, Vitamin B1 0.1mg / L, Vitamin B6 0.5mg / L, Niacin 0.5mg / L, Glycine 2.0mg / L, Serine 0.8mg / L;

[0066] Hormone: 2,4-D 3.4mg / L, KT 2.2mg / L;

[0067] Coagulant: vegetable gel 6.25g / L;

[0068] Active additives: coconut milk 15g / L, m...

Embodiment 2

[0084] In 2013, the lily cultivars Longya Lilium and Chuanlily were detoxified by using the two conventional lily detoxification methods of shoot apical meristem culture combined with heat treatment and shoot apex culture combined with ribavirin chemical treatment, and obtained Longya Lily respectively. Lily and Chuanlily virus-free seedlings, the frozen strains of Agroya lily and Chuanlily virus-free seedlings obtained by adopting the technical route of the present invention in Example 1 were used to detect three main viruses infecting lily: Cucumber Mosaic Virus (CMV) , Lily Asymptomatic Virus (LSV) and Lily Mottled Virus (LMoV), compare the difference in the detoxification effect of the technical route of the present invention and the conventional detoxification method on common lily viruses. The identification method adopts multiple RT-PCR technology (refer to "RT-PCR detection and detoxification technology research of three main viruses of lily" by Chen Jin et al.). The e...

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Abstract

The invention provides an anther culture detoxification method of edible lily. The anther culture detoxification method includes: (1), preparing an edible lily anther inducing culture medium; (2), selecting materials for inoculating anther; (3), disinfecting and inoculating the anther; (4), performing inducing culture on the anther; (5), performing embryonic callus differentiation and subculture; (6), hardening and transplanting edible lily bulbs; (7), removing edible lily haploid plants. Edible lily pollen plants are obtained through an anther culture path, and haploid abnormal plants in an anther cultural population are removed to obtain edible lily diploid plants; further lily virus detection of detoxification plants finds that the detoxification method almost realizes 100% detoxification, production experiments further find that both yield and quality of edible lily detoxification plants are improved greatly.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an anther culture and detoxification method for edible lilies that can efficiently remove lily virus through an anther culture approach. Background technique [0002] Lily is a monocotyledonous plant subclass Liliaceae Liliaceae ( Liliaceae ) Lilium ( Lilium ), which can be divided into medicinal lily, edible lily and ornamental lily (ornamental lily) according to the use. According to the "Pharmacopoeia of the People's Republic of China" (2005 edition one), Liliaceae Liliaceae Lily Lily ( Lilium lancifolium . Thunb.), Lily ( Lilium brownii F.E. Brownii var. viridulum Baker.), Lilium fineleaf ( Lilium pumilum DC.) for medicinal lily. The main varieties of edible lily in my country are Gansu Lanzhou lily, Hunan Shaoyang lily, and Jiangsu Yixing lily. The quality of Lanzhou lily is the best. There are three major varieties of lily for ornamental use in production and cultivat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 华小平钱振权
Owner 西诺(北京)花卉种业有限公司
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