Anther culture detoxification method of edible lily

A technology for edible lily and lily flower, applied in the biological field, can solve the problems of failing to meet production requirements, low cultivation efficiency, low efficiency of lily anther induction and the like

Inactive Publication Date: 2016-07-27
西诺(北京)花卉种业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] However, the existing edible lily anther culture efficiency is low, far from the

Method used

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  • Anther culture detoxification method of edible lily
  • Anther culture detoxification method of edible lily

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] In July 2012, the anthers of edible lily varieties Longya Lilium and Chuan Lily were selected for flower cultivation to induce detoxification. The detailed steps are as follows:

[0060] (1) Preparation of edible lily anther induction medium

[0061] Formulated per liter of distilled water from:

[0062] Macroelements: KNO 3 2600mg / L, NH 4 NO 3 1700mg / L, KH 2 PO 4 460mg / L, MgSO 4 ?7H 2 O370mg / L, CaCl 2 ?2H 2 O840mg / L;

[0063] Trace elements: MnSO 4 ?4H 2 O22.5mg / L, ZnSO 4 ?7H 2 O8.5mg / L, H 3 BO 3 6.25mg / L, KI0.825mg / L, CuSO 4 ?5H 2 O0.025mg / L, CoCl? 6H 2 O0.025mg / L, NaMoO 4 ?2H 2 O0.25mg / L;

[0064] Iron salt: Na 2 -EDTA?2H 2 O37.5mg / L, FeSO 4 ?7H 2 O27.5mg / L;

[0065] Organic ingredients: inositol 100mg / L, vitamin B10.1mg / L, vitamin B60.5mg / L, niacin 0.5mg / L, glycine 2.0mg / L, serine 0.8mg / L;

[0066] Hormone: 2,4-D3.4mg / L, KT2.2mg / L;

[0067] Coagulant: plant gel 6.25g / L;

[0068] Active additives: coconut milk 15g / L, mannitol 22g / L, hydro...

Embodiment 2

[0084] In 2013, the lily cultivars Longya Lilium and Chuanlily were detoxified by using the two conventional lily detoxification methods of shoot apical meristem culture combined with heat treatment and shoot apex culture combined with ribavirin chemical treatment, and obtained Longya Lily respectively. Lily and Chuanlily virus-free seedlings, the frozen strains of Agroya lily and Chuanlily virus-free seedlings obtained by adopting the technical route of the present invention in Example 1 were used to detect three main viruses infecting lily: Cucumber Mosaic Virus (CMV) , Lily Asymptomatic Virus (LSV) and Lily Mottled Virus (LMoV), compare the difference in the detoxification effect of the technical route of the present invention and the conventional detoxification method on common lily viruses. The identification method adopts multiple RT-PCR technology (refer to "RT-PCR detection and detoxification technology research of three main viruses of lily" by Chen Jin et al.). The e...

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Abstract

The invention provides an anther culture detoxification method of edible lily. The anther culture detoxification method includes: (1), preparing an edible lily anther inducing culture medium; (2), selecting materials for inoculating anther; (3), disinfecting and inoculating the anther; (4), performing inducing culture on the anther; (5), performing embryonic callus differentiation and subculture; (6), hardening and transplanting edible lily bulbs; (7), removing edible lily haploid plants. Edible lily pollen plants are obtained through an anther culture path, and haploid abnormal plants in an anther cultural population are removed to obtain edible lily diploid plants; further lily virus detection of detoxification plants finds that the detoxification method almost realizes 100% detoxification, production experiments further find that both yield and quality of edible lily detoxification plants are improved greatly.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to an anther culture and detoxification method for edible lilies that can efficiently remove lily virus through an anther culture approach. Background technique [0002] Lily is the general term for all species of the genus Lilium in the subclass Liliaceae (Liliaceae) of the monocotyledonous plant subclass Liliaceae. It can be divided into medicinal lily, edible lily and ornamental lily (ornamental lily) according to its use. According to the "Pharmacopoeia of the People's Republic of China" (2005 edition one), the dried fleshy scales of Lilium lancifolium. Thunb., Lilium brownii F.E. Browniivar. viridulum Baker. and Lilium pumilum DC. The leaves are medicinal lilies. The main varieties of edible lily in my country are Gansu Lanzhou lily, Hunan Shaoyang lily, and Jiangsu Yixing lily. The quality of Lanzhou lily is the best. There are three major varieties of lily for ornamental use in ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 王纪芝宋立胜尹纪岩
Owner 西诺(北京)花卉种业有限公司
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