Method for detecting mycoplasma pneumonia (MP) of sheep

Abstract

The invention which belongs to the technical field of biological detection relates to a method for detecting MP of sheep and provides the methylation state of CpG islands in an MBL (mannan-binding lectin) gene promoter region related with the MP of sheep and an application thereof. The invention also provides a method for detecting the MP of sheep and concretely relates to the methylation detection of the MBL promoter region and the artificial infection verification. The methylation state detection of the MBL gene promoter region of sheep is carried out through selecting a bisulfite sequencing process, the methylation state and the frequency of each CpG locus in a given region can be accurately detected, three substantially differential CpG methylation loci, CpG3, CpG4 and CpG5, related with the MBL level are found, a case that the MBL level after toxic elimination is lower than the MBL level before the toxic elimination is promoted, and the MBL gene promoter region methylation may be a more substantial characteristic of the MBL level reduction of serum. The method allows the MP infection of sheep and susceptible individuals to be sensitively and accurately detected, and provides complete information and scientific bases for the sheep MBL gene promoter region methylation and the MP infection research of sheep.

Description

Technical field: [0001] The invention belongs to the technical field of biological detection, and in particular relates to a methylation state of a CpG island in the promoter region of MBL gene related to Mycoplasma ovis pneumonia (MP) and its application, and also provides a method for detecting Mycoplasma ovis pneumonia. Background technique: [0002] At present, the study of gene promoter methylation in the epigenetic regulation mechanism is relatively in-depth, and it is closely related to gene expression. It is extensive and in-depth in the field of disease research. The main form of mammalian epigenetic expression regulation is DNA methylation modification. Refers to the methylation of the 5th carbon atom on the cytosine ring of a CpG island in the genome. This modification is reversible and heritable; includes promoters in normal tissues except for imprinted genes and inactive X chromosomes The CpG islands of most genes in the region are unmethylated or hypomethylated...

AI Technical Summary

Problems solved by technology

Because the disease is often chronically manifested after infection, the recovered sheep can carry bacteria for a long time, so the control and elimination of the disease are more difficult; the direct loss caused by Mycoplasma pneumoniae infection in sheep is the increased mortality of lambs, causing chronic nutrient consumption to the sheep industry , weight loss, delayed marketing, reduced reproductive rate and other indirect losses; at the same time, due to the lack of cross-immune protection between Mycoplasma ovis and other pathogens that can cause Mycoplasma pneumonia in sheep, it brings difficulties to immune prevention, so some anti-infection can be screened The gene of the disease has become the main way to prevent and control the disease;

Images