4 results about "Cpg island methylation" patented technology

The invention provides a construction method of a CpG island methylation phenotypic model for predicting tumor drug resistance and application of the CpG island methylation phenotypic model. Through the model provided by the invention, prediction of the tumor drug resistance CpG island methylation phenotype can be realized, and the model can be used for predicting the drug sensitivity and prognosis of tumor drug resistance patients and screening candidate drugs for treating the tumor drug resistance patients, provides actual support for further clinical research, and has a wide application prospect. And guidance and help are provided for future accurate personalized treatment.

The invention relates to the technical field of biomedicine, and particularly discloses a marker for breast cancer diagnosis, metastasis risk assessment and prognosis assessment and application of the marker. The marker comprises a full-length isomer ZNF132-L of the ZNF132 gene, a truncated isomer ZNF132-S of the ZNF132 gene, and the methylation level of a promoter CpG island of the ZNF132 gene. Wherein ZNF132-L and ZNF-S show opposite biological functions in breast cancer, ZNF132-F / ZNF132-S is used for breast cancer diagnosis, metastasis risk assessment and prognosis assessment, the diagnosis sensitivity reaches 88.06%, the specificity reaches 78.69%, the area under an ROC curve is 0.8566, MSP methylation detection and the ratio of ZNF132 L / S are combined, the diagnosis sensitivity can reach 96.88%, and the specificity is 81.25%. The invention further provides a specific primer pair and a kit for detecting the marker, breast cancer diagnosis and metastasis risk and prognosis evaluation can be sensitively and accurately realized, and a new strategy is provided for clinic.

The application belongs to the technical field of fluorescent quantitative PCR, and provides an enzyme digestion-fluorescent quantitative PCR detection method. The method comprises the following steps: step one, configuring a reaction mixture, wherein the reaction mixture comprises amplification reagents, a restriction endonuclease, Taq DNA polymerase, UDG enzyme, primers and a probe; and step two, setting a reaction program and a reaction system. Non-target templates in a qPCR reaction system are degraded by enzyme digestion of a restriction endonuclease, thereby reducing non-specific amplification; the proportion of target templates is increased, thereby avoiding the interference of non-target templates and improving detection sensitivity and detection stability. In particular, the method can effectively detect high GC content templates, such as TERT gene promoters or CpG island methylation.

There are currently no screening tests in routine use for oral and pharyngeal cancer beyond visual inspection and palpation. Described is an improve method of detecting oral and pharyngeal cancer with a saliva sample from a subject. The method includes identifying the presence or absence of a methylation classifier in the saliva the sample. The methylation classifier is determined by the methylation status of a panel of CpG islands in the sample. In an embodiment, the methylation classifier is determined by the methylation status of between 15 and 25 CpG islands, or alternatively, 22 CpG islands. The presence of the methylation classifier indicates a diagnosis of cancer in the subject. In an embodiment, the presence of the methylation classifier is determined with a bisulfite method of determining methylation status of the CpG islands.