Kit for detecting cervical carcinoma and detection method

Abstract

The invention belongs to the field of medicine, and particularly provides a kit for detecting cervical carcinoma and a detection method. The genes of SOX1, PAX1, LMX1A, NKX6-1, WT1 and ONECUT1 are tumor suppressor genes related to the cervical carcinoma, and the methylation of CpG islands of promoter sites of the genes is one of main reasons for the inactivation of the genes. In the kit and the detection method, a patient can be clearly determined whether to suffer from the cervical carcinoma or not by detecting the methylation of the CpG islands of the promoter sites of the genes of SOX1, PAX1, LMX1A, NKX6-1, WT1 and ONECUT1 respectively and detecting whether HPV-L1 exists or not, the methylation of the genes is treated in advance and outcome is detected by using the HPV-L1 proteins, so that the actual conditions of cervical canceration and outcome of the patient can be perfectly evaluated in terms of molecular level, treatment is effectively guided, and misdiagnosis, over-treatment and missed diagnosis in the conventional treatment of the cervical carcinoma can be further avoided.

Description

technical field [0001] The invention belongs to the medical field, in particular to a test kit and a detection method for detecting cervical cancer. Background technique [0002] Cervical cancer is currently the only cancer with a clear etiology, the most effective prevention, early detection and effective cure. Precancerous lesions and early cancers of the cervix are mostly asymptomatic, and it is difficult to find them in gynecological examination. When symptoms appear, more than 80% of them are invasive cancers, which are difficult to cure. [0003] It takes 5-20 years from HPV infection to cervical precancerous lesions. Today's cervical cancer screening: the first step is the Pap smear, and many factors may cause missed diagnosis. The second step is the human papillomavirus (HPV) test to detect whether women are infected with the virus and suffer from cervical cancer, which can also cause false positive results. Not only that, but it is also difficult to determine whet...

AI Technical Summary

Problems solved by technology

Cervical precancerous lesions and early cancers are mostly asymptomatic, and gynecological examination is also difficult to find. When symptoms appear, more than 80% of them are invasive cancers, which are difficult to cure

[0003] It takes 5-20 years from HPV infection to cervical precancerous lesions. Today's cervical cancer screening: the first step is to use Pap smear, and there will be many factors that cause missed diagnosis.

The second step is the human papillomavirus (HPV) test to detect whether women are infected with the virus and suffer from cervical cancer. This method can also cause false positive results.

Not only that, but it is also difficult to judge whether surgery should be performed and the period of surgery, which leads to unfavorable treatment and affects the treatment effect.

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