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Methylation high-flux detection method

A detection method, methylation technology, applied in biochemical equipment and methods, combinatorial chemistry, chemical library, etc., can solve the problems of reducing sequence capture efficiency, increasing the difficulty of probe design, limiting the breadth of the problem, and achieving a simple design method , Accurate analysis, capture the effect of high efficiency

Active Publication Date: 2014-06-18
BGI TECH SOLUTIONS
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Problems solved by technology

This method treats the genome with bisulfite before sequence capture, which increases the difficulty of probe design and reduces the efficiency of sequence capture and the coverage of the target region, thus limiting the breadth of application

Method used

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Embodiment Construction

[0030] Embodiments of the present invention will be described in detail below in conjunction with examples, but those skilled in the art will understand that the following examples are only used to illustrate the present invention, and should not be considered as limiting the scope of the present invention.

[0031] One aspect of the present invention provides a methylation high-throughput detection method, which includes the following steps: selection of sequence capture probes, library preparation, sequence capture, bisulfite treatment, computer sequencing and data analysis, wherein the sequence Capture and bisulfite treatment are performed between the adapter ligation step of library preparation and the PCR amplification and library purification steps by gel excision.

[0032] In a specific embodiment of the present invention, the probe in the methylation high-throughput detection method can be a probe for liquid phase or solid phase chip hybridization, preferably the length...

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Abstract

Provided is a high throughput methylation detection method, particularly a combined sequence capture and bisulfite sequencing method. The method accurately and effectively analyzes the methylation status of the target area in several samples simultaneously, lowers the difficulty of probe design, enhances operation and application feasibility, and enables high throughput methylation detection of high accuracy on interested target sequences and areas in a complete genome. The method is targeted and conserves energy and time.

Description

technical field [0001] The invention relates to the field of high-throughput genome methylated DNA detection, and in particular provides a method combining sequence capture and bisulfite sequencing. In addition, the present invention also relates to the technical fields of exome sequencing and methylation analysis sequencing based on bisulfite conversion. The invention also relates to a methylation label (Index) linker technology, which can realize simultaneous capture of several samples on one chip. The method of the present invention is particularly suitable for the second generation sequencing technology, especially the solexa sequencing technology. Background technique [0002] DNA methylation is one of the hottest directions in the field of epigenetics research, and it is gradually becoming an epigenetic marker for various diseases such as mammalian development and cancer. DNA methylation not only plays an important role in the modification of chromatin structure and ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68C40B50/06C40B40/08
CPCC12Q1/6874C12Q1/6851C12Q1/6869C12N15/1065C12Q1/6806C40B50/06C12Q2523/125C12Q2525/191C12Q2535/122C12Q2537/159
Inventor 罗慧娟吉冠玉蒋慧吴明枝孙继华吴仁花王君文高飞
Owner BGI TECH SOLUTIONS
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