2 results about "Re sequencing" patented technology

The application discloses a method and system for correcting Hi-C sequence alignment by fusing DNA methylation information, and the method comprises the following steps: obtaining a preliminary alignment result of Hi-C sequencing of a sample genome relative to a reference genome and a methylation site map; obtaining a corresponding candidate alignment position and an original sequence alignment score of each read pair; positioning an alignment interval on the reference genome at both ends of each candidate alignment position; based on the methylation site map, counting the number of methylation sites covered at both ends of the candidate alignment position to obtain a methylation penalty score representing biological consistency; obtaining a recalibration comprehensive score based on the methylation penalty score, reordering all candidate alignment positions of the read pair, calculating an alignment quality update value of each candidate position, and outputting a corrected alignment result. The application solves the problem that in a polyploid and a highly repetitive genome, multiple alignment of Hi-C reads cannot accurately determine the real source position.

A method and apparatus for decompressing FASTQ data through character frequency-based sequence reordering implemented by a computer apparatus, the method including separating genome sequencing data into components of an identifier, a nucleotide sequence read, and prediction quality information; measuring character frequency for the entire data of each of the nucleotide sequence read and the prediction quality information; producing a score by applying the measured character frequency for the nucleotide sequence read and the prediction quality information; reordering the nucleotide sequence read and the prediction quality information based on a condition that is preset based on the score; and compressing at least one of information of the identifier, an identifier of the nucleotide sequence read, and an identifier of the prediction quality information through a compression program by including the reordered nucleotide sequence read and the reordered prediction quality information and generating compressed genome sequencing data.