185 results about "Re sequencing" patented technology

The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.

The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.

The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences using adaptors interspersed in target polynucleotides. The sequence information can be new, e.g. sequencing unknown nucleic acids, re-sequencing, or genotyping. The invention preferably includes methods for inserting a plurality of adaptors at spaced locations within a target polynucleotide or a fragment of a polynucleotide. Such adaptors may serve as platforms for interrogating adjacent sequences using various sequencing chemistries, such as those that identify nucleotides by primer extension, probe ligation, and the like. Encompassed in the invention are methods and compositions for the insertion of known adaptor sequences into target sequences, such that there is an interruption of contiguous target sequence with the adaptors. By sequencing both “upstream” and “downstream” of the adaptors, identification of entire target sequences may be accomplished.

The disclosure provides methods of generating paired reads in sequencing-by-synthesis process, particularly, in systems with relatively short read lengths (e.g., 15-35 bases), such as for example, in single molecule sequencing by synthesis. Several implementations of the methods are provided. Of particular advantage are the methods that permit re-sequencing of the template, which yields lower error rates. The invention further provides methods of using paired reads, for example, for positioning them over repeats or for assembly into large sequences, including whole genome assembly.

A system and method are described for greatly increasing the number of services that can be encrypted with existing conditional access equipment. The method is most useful when many digitally compressed programs are encrypted at the same time. Only the most critical components of each compressed video, audio, or data stream are selected and then sequenced into a single stream. Additional formatting causes this sequence of segments from multiple sources to appear as a single continuous stream to the conditional access system. Once this stream has been encrypted, it is demultiplexed and the components are restored and re-sequenced into their respective programs. Messages such as the Entitlement Control Messages that are inserted into the stream by the encryption system, are also adjusted and included with each of the reconstructed programs. The technique not only allows encryption systems to be designed using less encryption hardware, but also simplifies the management of encryption sessions, particularly in on-demand programming applications.

The present invention provides a method and apparatus for re-sequencing data packets received from multiple devices in a packet-based digital communication system. A set of data packets is received by a data packet sequencer. Each data packet includes an indicator of the originator of the data packet. The data packet sequencer stores the set of data packets, preferably in an input buffer. The data packet sequencer reorders the data packets by retrieving a first data packet and determining the originator of the first data packet. The data packet sequencer then transmits all data packets that were originated by a first originator of the first data packet prior to transmitting data packets that were not originated by the first originator.

A method and apparatus for creating a trick play video display from an MPEG-2 digital video transport stream are described. A trick play transport stream frame sequence template for each transport stream frame sequence and trick play video display mode supported by a video recorder is stored in the recorder. When the recorder receives an input transport stream frame sequence, the sequence is identified and template corresponding to that sequence and the selected trick play display mode is selected. The template is used to identify frames of the input transport stream to be appended to the trick play display transport stream frame sequence. When the trick play transport stream frame sequence is constructed the program clock reference, presentation time stamps and decoding time stamps associated with the frames are updated from information in the template.

Methods, apparatuses and systems facilitating the aggregation or bonding of communications paths into a higher-bandwidth, logical communications path. Embodiments of the present invention can be applied to bond different physical links (e.g., xDSL over twisted pairs), different channels on the same physical line, or even different channels or frequencies in a wireless communications network. The present invention further provides methods, apparatuses and systems facilitating the re-sequencing of data flows transmitted across bonded communications paths. In one embodiment, the re-sequencing methodology of the present invention adapts to the transmission delays (both absolute and relative) across the bonded communications path.

A system and apparatus for enabling the end user to schedule the display of online videos and dynamically displaying online videos according to a user preference. The system provides an editorially-selected guide to online videos in which videos from existing online video providers are gathered under a single portal and thereby provides the end user a directory through which the user can view recommended videos as presented by the system, or personalize the selections into a dynamically-generated subset of videos. The system also provides a seamless playlist of multiple videos from various websites, and allows the user to manage and re-sequence this playlist.

The disclosure provides methods of generating paired reads in sequencing-by-synthesis process, particularly, in systems with relatively short read lengths (e.g., 15-35bases), such as for example, in single molecule sequencing by synthesis. Several implementations of the methods are provided. Of particular advantage are the methods that permit re-sequencing of the template, which yields lower error rates. The invention further provides methods of using paired reads, for example, for positioning them over repeats or for assembly into large sequences, including whole genome assembly.

A novel apparatus and method of packet re-sequencing applicable to systems wherein packets are assigned sequence numbers and transmitted over multiple channels with the requirement they be re-ordered at the receiving side. The mechanism is particularly suitable for use in cable systems adapted to implement the DOCSIS 3.0 specification which permits the bonding of a plurality of downstream channels into a single virtual high data rate pipe. In operation, received packets are stored in a memory whereby a pointer to the memory storage location is written into a context table diagram in accordance with the sequence number extracted from the packet. Packets are released in sequence order regardless of the order in which they were received.

The invention discloses a video recommendation method and device. The video recommendation method disclosed by the invention comprises the following steps: according to historical recorded information of videos watched by users, obtaining initial user preference parameters and a plurality of to-be-recommended videos sequenced according to the recommendation level; according to the recommendation level, selecting the first to-be-recommended video from the current to-be-recommended videos and writing the first to-be-recommended video in a recommendation list; according to a feature vector and the user preference parameters of the first to-be-recommended video, obtaining user preference satisfaction degrees by calculation; correcting the user preference parameters according to the user preference satisfaction degrees, and re-sequencing the to-be-recommended videos which are not written in the recommendation list according to the corrected user preference parameters; recommending the to-be-recommended videos in the recommendation list to users according to the sequence of the to-be-recommended videos written in the recommendation list. According to the method and the device, the user preference parameters are dynamically corrected according to the user preference satisfaction degrees calculated in real time; the singularity problem of the video recommendation is solved.

The invention discloses a short text query expansion and indexing method based on a word vector. The short text query expansion and indexing method particularly comprises: A, pretreatment of corpus information of a short text; B, expression of every word in a corpus dictionary by the word vector through a training model; C, query extension; D, obtaining of a text candidate set through a query expansion word set and a BM25 index model; E, extraction of subject of the short text; F, calculation of the text vector of the short text; G, re-sequencing of the short text returned by a traditional indexing model. The short text query expansion and indexing method can more exactly and effectively satisfy the indexing demand of a user; moreover, the query expansion module can find out words capable of expressing user's intension according to the existing data so as to perform the query expansion.

The present invention is related to a method and system for interlayer control where a Protocol Data Unit (PDU) is received from a lower protocol layer at a upper layer where the lower and upper layer are both involved in a retransmission function, a re-sequencing function, or both functions. The lower layer signals the upper layer for retransmission of a PDU when the retransmissions of that PDU have been exhausted and failed at the lower layer. The signaling scheme from the lower layer to the upper layer is an ABORT notification. This ABORT notification comes with associated parameters to indicate to the upper layer which PDU can be requested for retransmission from the upper layer.

The present invention relates to pathogen detection and identification by use of DNA resequencing microarrays. The present invention also provides resequencing microarray chips for differential diagnosis and serotyping of pathogens present in a biological sample. The present invention further provides methods of detecting the presence and identity of pathogens present in a biological sample.

The invention relates to a commodity property clustering method based on user comments, and belongs to the field of data mining. The method comprises the following steps: combining user comments information and commodity property information; calculating the feedback rates of the properties of commodities with similar characteristics according to the user comments; calculating the similarity of the commodities by taking the feedback rate as a weight; re-sequencing the commodities; returning needed commodities comprising real information to a user. By adopting the commodity property clustering method, commodities with most real good comments can be directly screened in concerned commodity properties of the user, so that the commodity selecting and purchasing time is saved, and the shopping experience of the user is improved.

A method and apparatus for synchronizing audio data to text data in which the text is manually or automatically divided into an arbitrary number of sections and a text section is selected for display and presented to a narrator for audio input. A human narrator reads the displayed selected section of text into a computer to form an audio data portion for the selected section that is linked to the text by entering synchronization marks to the computer. The sections of text may be selected for reading and audio input in any order and by any number of narrators. Each recorded audio portion is converted to an audio data file and the audio data files are re-sequenced to conform to the original order of the text based on a playback control file. A plurality of narrators may contribute audio data from separate workstations of a network, or workstations which are connected to a central server over a communications network, such as the Internet. After the sections of text and accompanying audio have been properly sequenced and assembled into a single audio / text program, it is stored on a portable computer-readable medium (e.g. a CD-ROM) and a user may select from the playback control file any portion of the text for view and / or select any portion of viewed text and hear the audio portion corresponding thereto.

The invention discloses a processing method and device of a search result. The method comprises the following steps: acquiring a current search word received in a search box of a client; inquiring to obtain a search result list according to the current search word; returning the search results in the search result list to be displayed on the client, and then monitoring an operation behavior corresponding to the search result; extracting a tag of the search result having the operation behavior, and calculating an online weight value of each tag; inquiring from a historical search log to obtain a historical search result list corresponding to the current search word, and acquiring a historical tag weight value corresponding to the tag of each historical search result; re-sequencing the tags of the search results according to the online weight value and the historical tag weight value, and generating a recommend tag list; and pushing a search result corresponding to the current search word to the client according to the recommend tag list. By adopting the method and device, the technical problem of the prior art that an accurate search result cannot be obtained by virtue of the search method.

The software development tool (701) forms part of a software development kit (700). The software development tool (701) receives as input object files (606) and library files (607) and subjects the object file instructions and data definitions of the object files and the library files to re-sequencing to generate new object files and the links between them which are semantically equivalent to the input object files and library files and which are used to generate an optimized executable. The software development tool (701) is capable of automatically generating an executable, without requiring any modification of the source code, which is optimized to execute more deterministically and with respect to execution time; reduced processor and memory requirements; reduced off-chip memory accesses; reduced memory latency.

Described herein is a buffer concentration for highly efficient ligation of two oligonucleotides. The embodiments herein have led to the development of an optimized ligation step used in the sample preparation for sequencing reactions. Further, embodiments herein describe a high-throughput method for sequencing using barcodes or the purpose of multiplexing several samples simultaneously and novel methods for making targeted DNA libraries for re-sequencing on massively parallel next-generation sequencing platforms and for alternatives to gel-purification for recovering the desired templates from small RNA libraries for next generation sequencing.

The invention belongs to the Internet finance and block chain technical field and provides a crowd funding lotting method. The method includes the following steps that: the support of crowd funding succeeds, each lot of the crowd funding is numbered according to the sequence of the crowd funding; at least one related parameter of a block chain after a certain time of the success of the support of the crowd funding is selected; the sequence of disordered serial numbers of the crowd funding is obtained; and the disordered serial numbers of the crowd funding are selected sequentially according to the target number of the crowd funding so as to be adopted as lotted serial numbers. The invention also provides a lotting method system supporting the success of crowd funding. The system includes a crowd funding numbering module, a related block chain parameter selection module, a re-sequencing module and a lotted serial number selection module. According to the crowd funding lotting method and system of the invention, the justification and supervision of a third party are not required in a whole lotting process, and the whole lotting process can be justified, open and fair. The algorithm in the invention is simple and convenient.

A re-sequencing system offloads the cycle intensive task of re-sequencing TCP packets from host memory using a partial offload engine to re-sequence out-of-sequence data packets. However, as opposed to re-ordering the actual data packets, no actual data copy is needed. Instead, packet descriptors associated with each data packet are generated, and it is the packet descriptors that are re-sequenced. The data packets themselves are temporarily stored in packet buffers while the packet descriptors are sorted into sequence. The re-sequencing system preferably re-sequences a data stream of TCP data packets received from an ethernet network. The re-sequencing system is implemented within a computing device, preferably a personal computer or a server.

High-throughput detection for the interesting base or the mutation site in the nucleic acid sample can be achieved by means of the linear test probe pairs P1 and P2. The test probe pairs P1 and P2 respectively comprise either of the flanking complementary sequences which are adjacent to the interesting base or the mutation site in the nucleic acid sample. The invention can be applied to the re-sequencing the target nucleic acid sequence, the detection and analysis for the mutation, insertion, or deletion sites of a known nucleic acid sequence, and the genotyping of the pathogenic microorganism.

This invention provides a transmission method, devices and data exchange device for high speed data flows, in which, the method includes: A, receiving a high speed data flow and adding sequence number for every packet according to the receiving order and distributing the packets and the sequence numbers to N parallel transfer channels, among which, N is greater or equal to 2, B, utilizing the channels to transmit said data packets and the numbers, C, receiving the data packets and the numbers and sequencing the packets to turn them into a high speed data flow and output, which realizes high speed single flow line speed transfer by distributing data packets and re-sequence.

A novel apparatus and method of packet re-sequencing applicable to systems wherein packets are assigned sequence numbers and transmitted over multiple channels with the requirement they be re-ordered at the receiving side. The mechanism is particularly suitable for use in cable systems adapted to implement the DOCSIS 3.0 specification which permits the bonding of a plurality of downstream channels into a single virtual high data rate pipe. In operation, received packets are stored in a memory whereby a pointer to the memory storage location is written into a context table diagram in accordance with the sequence number extracted from the packet. Packets are released in sequence order regardless of the order in which they were received.

A system and method are described for greatly increasing the number of services that can be encrypted with existing conditional access equipment. The method is most useful when many digitally compressed programs are encrypted at the same time. Only the most critical components of each compressed video, audio, or data stream are selected and then sequenced into a single stream. Additional formatting causes this sequence of segments from multiple sources to appear as a single continuous stream to the conditional access system. Once this stream has been encrypted, it is demultiplexed and the components are restored and re-sequenced into their respective programs. Messages such as the Entitlement Control Messages that are inserted into the stream by the encryption system, are also adjusted and included with each of the reconstructed programs. The technique not only allows encryption systems to be designed using less encryption hardware, but also simplifies the management of encryption sessions, particularly in on-demand programming applications.

A method of manipulating manufacturing features in horizontally structured manufacturing process models for large parts, comprising: selecting a manufacturing feature from a plurality of manufacturing features; wherein the horizontally structured manufacturing process model includes a first master process model in a first file with an established coordinate system with an existing sequence of virtual machining each manufacturing feature of the plurality of manufacturing features into a virtual blank, and each manufacturing feature exhibiting an associative relationship with the coordinate system and the virtual blank. The method also includes relocating the selected manufacturing feature of another master process model; and re-sequencing the master process model to establish a new sequence for virtual machining each manufacturing feature of the plurality of manufacturing features into the virtual blank indicative of the relocating.

The invention discloses a code morphing-based binary code obfuscation method. The method comprises the following steps: 1, determining a morphing template library; 2, detecting a PE (Portable Execute) file; 3, locating a key code segment; 4, disassembling the key code segment to obtain an assembly instruction sequence, identifying a circular structure in the code segment, and calculating the weight of each instruction in the key code segment; 5, morphing all assembly instructions in the assembly instruction sequence; 6, slicing the assembly instruction sequence and re-sequencing; 7, assembling a new assembly instruction sequence generated in the step 6, and converting into a binary code; 8, reconstructing the PE file. In the method, a computer system is used for obfuscating the executable binary code file under a windows system, so that high protection strength is achieved, and extension becomes easy.

The invention discloses a correlation-quality sequencing image retrieval method based on tag retrieval. The correlation-quality sequencing image retrieval method comprises the following steps of: firstly, automatically sequencing social images according to the correlation of images and tags, fusing the vision consistency among the images and the vision consistency of the semanteme correlation among the images and the tags by utilizing a normalization frame, and solving an optimization problem by utilizing an iteration algorithm so as to obtain a correlation sequence; and selecting the brightness, the contrast ratio and the color variety of the images to be used as quality characteristics of the images to balance previously returned images, overlaying correlation scores and quality scores by utilizing a linear model, and re-sequencing the images according to a final total score, and therefore, a correlation-quality sequencing algorithm is realized.