Oxidative damage DNA detection method

A technology of oxidative damage and buffer solution, which is applied in the fields of biochemical equipment and methods, and the determination/inspection of microorganisms. Achieve the effect of low detection limit, high sensitivity and easy promotion
CN106939336AInactive Publication Date: 2017-07-11CHINA INST OF SPORT SCI
2 Cites 4 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINA INST OF SPORT SCI
Publication Date
2017-07-11
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention discloses an oxidative damage DNA detection method, and relates to the technical field of DNA oxidative damage detection. The method comprises (a) obtaining of DNA containing 3 ', 5'-phosphate group nucleotide voi, to be more specific, adding a DNA repair enzyme for recognition and cutting of oxidized DNA bases; (b) introduction of hydroxyl into 3 ', 5' site, to be more specific, adding alkaline phosphatase for dephosphorylation to obtain the DNA containing 3 ', 5'-hydroxyl group nucleotide voids; (c) introduction of a fluorescent marker, to be more specific, adding DNA polymerase I and the fluorescent marker to obtain fluorescence-labeled DNA; (d) formation of a PFP-DNA complex, to be more specific, adding PFP to obtain the PFP-DNA complex; and (e) oxidative damage DNA detection, to be more specific, detecting damage DNA by FRET. The method alleviates the disadvantages of tedious steps, low sensitivity and low selectivity of DNA damage detection in the prior art. The method has the advantages of high sensitivity, strong specificity and high accuracy.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention relates to the technical field of DNA oxidative damage detection, in particular to a method for detecting oxidative damage to DNA. Background technique

[0002] DNA (deoxyribonucleic acid, deoxyribonucleic acid) is the carrier of eukaryotic genetic information, and its integrity is crucial to the survival and normal reproduction of organisms. DNA oxidative damage plays an important role in the formation of diseases and human aging. Studies have found that DNA oxidative damage is related to Alzheimer's disease, Parkinson's disease, diabetes, metabolic syndrome, atherosclerosis and other aging diseases. The mechanism of self-initiated DNA damage is mainly due to the oxidative phosphorylation during the generation of ATP by the aerobic respiration of the cell mitochondria. Oxygen is reduced to water as the final electron acceptor. In the process of electron transfer, electrons combine with oxygen molecules to generate superoxide free base, ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More