3 results about "Microbial protease" patented technology

The present invention relates to microbial proteases for cell detachment. The present invention also relates to compositions suitable for cell detachment comprising said microbial proteases, use of said microbial proteases in cell detachment processes, and methods of cell detachment employing said microbial proteases.

This invention discloses a method for preparing and applying a microbial protease liquid fertilizer, comprising the following steps: S1. Mixing animal and plant protein raw materials with water, and subjecting them to physical-assisted cell wall disruption to obtain a pretreated protein slurry; S2. Inoculating with a composite seed liquid, and performing aerobic fermentation and enzymatic hydrolysis at 45-50℃ for 48-60 hours, maintaining the pH of the fermentation broth at 6.5-7.5 to obtain an enzymatic hydrolysate; S3. Cooling to 28-35℃, inoculating with a mixed probiotic agent, and performing anaerobic or facultative anaerobic fermentation for 48-72 hours to obtain a fermentation broth; S4. Adding trace element salts, and stirring the reaction at 40℃ to 45℃ for 1.5-2.5 hours to allow small molecule active peptides in the fermentation broth to undergo in-situ complexation with metal ions, followed by post-treatment to obtain the microbial protease liquid fertilizer; Through the synergistic effect of physical cell wall disruption and composite enzymatic hydrolysis, the conversion efficiency of recalcitrant waste protein raw materials such as feather meal is significantly improved.

The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.