44 results about "Microbial protease" patented technology

The invention relates to a method for preparing peanut protein polypeptide by microbial fermentation, including the steps as follows: (1) peanut protein preparation; (2) polypeptide preparation; (3) microbial fermentation; (4) separation; (5) finished product formation. The method adopts the process of complex enzyme hydrolyzation to simultaneously realize certain degree of zymohydrolysis as well as modification during zymohydrolysis so that the flavor of the zymohydrolysis product, i.e. the peanut polypeptide can be improved and the peanut polypeptide can be used as a raw material for food addiitive or nutrient protein. Compared with animal prolease and plant prolease, the microbial protease enzyme adopted by the method has the characteristics of good solubility, high activity and strong specificity, and can deeply hydrolyze the protein.

The invention discloses a method for mixing natural rubber by a wet process. The method will be widely applied in rubber products' filling materials and processing aids such as carbon black, white carbon black, potter's clay, calcium carbonate and other hardly-dispersed chemical materials. The method contains the following steps of: adding a dispersant, an antioxidant, an anti-sinking agent, a wetting agent and water to prepare a mixture with its solid content being 10-50%, grinding for 48-72h, carrying out pretreatment at 90-95 DEG C for 4h to prefabricate a dispersion, uniformly mixing the dispersion with condensed latex, adding microorganism protease and acetic acid, followed by coagulation, washing, and drying to prepare a mixed stock. As the chemical filling material has good dispersing uniformity in the rubber, comprehensive performance of the mixed stock is effectively raised.

The invention belongs to the technical field of feeds, enzymic preparations and microorganisms, and in particular relates to a preparation method of an acidified small-peptide protein feed. The preparation method comprises the following steps: mixing soybean meal, rice protein, wheat protein, potato protein and water; performing size mixing, sterilizing and cooling so as to prepare a fermentation broth; adding bacillus subtilis strain liquid and saccharomycetes strain liquid to the fermentation broth to perform aerobic fermentation and enzymolysis on the liquids; carrying out solid-liquid separation to obtain small-peptide protein fluid and a protein solid content wet basis; concentrating small-peptide protein fluid to obtain small-peptide protein size; mixing the protein solid content wet basis, a protein feed, lactic acid bacteria strain liquid and microorganism protease; performing solid anaerobic fermentation and enzymolysis to obtain fermentation protein residue; absorbing and uniformly mixing the small-peptide protein size, the fermentation protein residue and lactic acid; and then drying. The acidified small-peptide protein feed is rich in small peptide, lactic acid, yeast protein, probiotics and other small molecular nutrients, and has the characteristics of being balanced in amino acid, high in nutrition, easy to digest, high in palatability, low in content of antinutritional factors, and the like.

The invention relates to a method for preparing rubber powder/natural rubber liquid-phase mixed compound rubber. The method includes steps of (1), mixing and stirring rubber powder (with the size of 30-200 meshes), water, anti-sedimentation agents and antioxidants to obtain stable rubber powder dispersion; (2), mixing and stirring latex with the solid content of 20%-70%, water, microorganism protease and stabilizers to obtain pretreated latex; (3), adding the rubber powder dispersion and the pretreated latex into a stirring machine according to a proportion of 1:10-1:0.1, mixing and stirring the rubber powder dispersion and the pretreated latex, adding coagulating agents into the rubber powder dispersion and the pretreated latex, stirring the coagulating agents, the rubber power dispersion and the pretreated latex until mixed rubber is sufficiently coagulated; (4), washing the coagulated mixed rubber, squeezing and dewatering the mixed rubber and drying the mixed rubber to obtain the rubber powder/natural rubber liquid-phase mixed compound rubber. The method has the advantages that the dispersion uniformity of the rubber powder in natural rubber can be improved, destruction of molecular chains of the natural rubber when the rubber powder and the natural rubber are mixed with each other under warming mixing conditions can be reduced, energy consumption can be reduced, and the rubber powder/natural rubber liquid-phase mixed compound rubber which is a composite material prepared by the method is excellent in physical and mechanical property.

The invention discloses a leather softening compound enzyme with acid proteases and a method for preparing the leather softening compound enzyme. The leather softening compound enzyme and the method have the advantages that the leather softening compound enzyme is mainly made of the high-activity acid proteases with abundant plant proteases and microorganism proteases, mould culture, acid lipase, non-ionic surfactants, plant extract, antioxidants, protective agents, activators and the like are scientifically compounded, accordingly, the compound enzyme is provided with complete enzyme systems, is stable in activity and high in efficacy, permeability and degreasing capacity and can act mildly and moderately, the hand feel and physical and mechanical properties of crust leather can be obviously improved, the bare weight of the crust leather can be obviously increased, and the shrinkage temperatures of the crust leather of raw hide of wet-blue leather of different animals can be increased by 9-14 DEG C; the porosity of the crust leather can be improved by 7.85-10.96%; the breathability of the crust leather can be improved by 41.0-50.1 mL/cm<2>hr; the tensile strength of the crust leather can be improved by 6.51-7.47 N/mm<2>; the tear strength of the crust leather can be improved by 5.28-20 N/mm; the elongation of the crust leather can be improved by 4.8-8.7%; the quality of products can be greatly improved, and the production cost can be reduced.

A dried soybean polypeptide powder with hypolipemic function is prepared through adding distilled water to separated sobyean protein, grinding, adding neutral microproteinase, regulating pH value and temp., hydrolyzing, adding pancreatin, regulating pH value and temp., hydrolyzing again, neutralizing, boiling for deactivating enzyme, cooling, filtering, vacuum concentrating and spray drying. Its advantages are high activity and excellent hypolipemic and antioxidizing functions.

The present invention provides methods for protein engineering. Specifically, the invention provides methods utilizing site evaluation libraries to design libraries that optimize two or more properties of a protein. The present invention also provides variant subtilisins suitable for various uses.

The invention belongs to the technical field of papermaking, and particularly relates to a plant fiber treatment and preparation method for papermaking. The method includes the following steps of plant-fiber opening, plant-fiber softening with a softening enzyme, plant-fiber purification, purified plant-fiber crushing, chemical softening, lignin removal and other related operation steps, wherein plant fiber includes wood chips or bamboo chips or straw or bagasse, and the softening enzymes includes industrial trypsin, microbial protease and compound enzyme. The plant fiber treatment and preparation method for papermaking changes the method, including mechanical crushing and hydrothermal treatment or chemical soaking, of the prior art, enzymolysis, softening and mechanical crushing are performed in sequence, the use amount of alkali, hydrogen peroxide and the like in the chemical treatment process is reduced, the grinding energy consumption and the grinding electricity consumption are reduced, splitting and fibrillating are easy to occur at the time of plant fiber grinding, the strength of obtained pulp is improved, the paper strength is increased, the whiteness of paper pulp is improved and yellowing is inhibited.

The invention discloses an efficient post-extraction method of curdlan. The preparation method comprises the following steps: firstly, adding a recombinant microbial protease DSNJ-rPE into a fermentation broth producing curdlan agrobacterium spp, and carrying out reacting at 20-45 DEG C for 1-2 hours; carrying out filtering and collecting thallus, and dissolving the thallus by using an alkali solution to obtain curdlan; then, carrying out centrifuging or plate-frame filtration to remove the thallus; neutralizing the supernate by an acid solution to separate out curdlan reversible gel; washingthe gel with deionized water for 2-4 times, carrying out centrifuging or plate-frame filtration, collecting the colloid, and cutting the colloid into particles by a chopper mixer; carrying out quick-freezing treatment on the granular colloid by liquid nitrogen or dry ice; dehydrating and drying the granular colloid by adopting a boiling drying method; and finally, further crushing the dried colloidal particles to obtain the curdlan refined product. According to the method disclosed by the invention, production cost can be reduced, and the polysaccharide recovery rate and production efficiencycan be improved. Meanwhile, gel strength of the curdlan extracted by using the method is higher than gel strength of curdlan extracted by a conventional method.

The invention discloses a production method of feed from wheat distillers' grains by a non-drying method and the feed, and relates to the field of feed processing. The method is characterized in thatby a microbial fermentation technique, high-moisture distillers' grains and a large amount of another by-product bran in production of wheat alcohol are mixed, and a bacillus subtilis KG109 and bacillus subtilis R-02 combined fermentation method is used for producing fermented wheat distillers' grain feed with the moisture content of about 35%. Compared with the prior art, the product needs not drying and can be stored for a long term before being unpacked, so energy consumption of drying is saved, high expense for drying is avoided, and the defects of protein denaturalization and reduction ofutilization ratio caused by high-temperature drying are overcome. Owing to the effects of microbial protease, protein in partial distillers' grains is degraded into small peptides with biological activity, thereby achieving an immunological enhancement effect on fed animals.

The invention belongs to the bioengineering field, and discloses a method for producing animal chondroitin sulfate. An animal by-product, namely cartilage, is used as a raw material; the method comprises the following steps of: performing hot alkali dissolution; performing ultrasonic extraction to improve the extraction efficiency; regulating acid, performing double enzymolysis by adopting plant protease and microbial protease to reduce the production cost while improving the yield of a product; removing impurities and decoloring, and performing further precipitation by using ethanol to improve the purity of the product; and finally, performing centrifugal drying, crushing, and packing to obtain the chondroitin sulfate finished product. The chondroitin sulfate produced by the production process provided by the invention has the characteristics of high extraction efficiency, stable quality, short production time, and change from waste to treasure; at the same time, a nutritious feed is prepared, no any waste is produced, environmental pollution is avoided, industrial energy consumption is reduced, and the chondroitin sulfate can be applied to large-scale industrialized production.

The present invention provides a novel proteolytic enzyme composition more particularly an orally administered proteolytic enzyme composition comprising of one or more acid proteases, one or more alkaline protease and one or more plant proteases. More particularly, the composition comprises of microbial (fungal, bacterial or other microbes) protease enzymes, proteases from plant and animals proteases thereof.

An optimized method for obtaining ACE activity inhibitory peptides includes the steps of whey ultrafiltration for obtaining a whey concentrate, enzymatic hydrolysis, hydrolysate ultrafiltration and inhibitory peptide separation. The method includes selecting and adding a mixture having suitable proportion and amount of two microbial proteases and having complementary action to the whey concentrate for the hydrolysis thereof in order to obtain peptides with an ACE inhibition percentage greater than 70% and an IC₅₀ index of 30 to 100 μg/ml.

Provided is a method for preparation of an acaudina leucoprocta collagen antioxidant peptide by enzymolysis with microbial protease. The method comprises the steps: selecting acaudina leucoprocta as araw material, and removing heavy metals and miscellaneous proteins by soaking with an acid, an alkali and EDTA, to obtain collagen; with assisting of ultrasonic waves, carrying out enzymolysis by collagenase, to obtain a collagenase solution, adding glucose or sucrose to the collagenase solution, and adjusting the pH to 6-7, to obtain an initial fermentation liquid; adding bacillus geocompostingor bacillus lichenibacillus or bacillus megigantobacilli into the initial fermentation liquid, and carrying out sealed fermentation to obtain a fermentation filtrate; carrying out freeze drying of thefermentation filtrate or removing macromolecules by a tubular membrane, and carrying out intercept classification, concentration desalting and spray drying to obtain the acaudina leucoprocta collagenpeptide; and separating and purifying the collagen peptide by a membrane separation, gel filtration and reverse-phase high-performance liquid chromatography technology, and screening to obtain the collagen antibacterial peptide product.

The invention discloses a method for preparing Acaudina leucoprocta collagen oligopeptide through hydrolysis with combination of collagenase and novel microbial protease. The method comprises: pretreatment, collagen extraction, fermentation, microbial protease purification, enzymolysis, microbial protease enzymolysis, and purification. According to the present invention, during the enzymolysis, the dual-frequency ultrasonic wave assisted enzymolysis is used, such that the energy barrier between the ground state and the active state of the hydrolysis reaction can be reduced, and the Ea value and the KM value of the enzymolysis reaction can be reduced so as to increase the affinity between the matrix and the enzyme, greatly increase the enzymatic reaction rate, and save the time cost and thelabor cost; after the Acaudina leucoprocta collagen is hydrolyzed by combining the collagenase and the novel microbial protease, the collagen is hydrolyzed into the amino acids with low molecular weight and the oligopeptide with low molecular weight so as to be easily absorbed by the body and easily provide the medical health effects of the Acaudina leucoprocta collagen oligopeptide; and the obtained product is the nutrition health substance with characteristics of easy absorption, high nutrition and no toxic-side effect.

The invention relates to a vegetable fungi apozymase and a preparation method. The vegetable fungi apozymase is prepared by adding sugar water into vegetable raw materials, adding sugar water into fungi raw materials and then naturally fermenting. The vegetable fungi apozymase is characterized in that the vegetable raw materials are composed of radish, cabbage, leaf mustard, celery, carrots, tomato, eggplant, wax gourd, balsam pear, towel gourd, pea, broad bean, green soy bean, garlic, Chinese chive, lettuce, spinach, lotus roots and potato respectively, and the fungi raw materials are composed of agaric, tremella, lucid ganoderma, lentinus edodes, oyster mushroom, straw mushroom and carpophorum Hericii Erinacei; bearded tooth carpophore respectively. The apozymase having the effects of preventing and treating insomnia and amnesia is obtained according to the steps of fermenting rich nutrients contained in the vegetable raw materials into fruit raw material fermented liquid rich in nutrients and microorganism protease, and then fully mixing with fungi raw material fermented liquid and fermenting. Full blending of active ingredients in vegetables and nutrients in fungi raw materialfermented liquid is guaranteed, so that the vegetable fungi apozymase can be easily absorbed by a human body.

The invention discloses a preparation method for a biological microbial protease preparation. The preparation method comprises the following steps: culture and collection of thalli; material preparation and granulation; etc. The invention has the following advantages: the preparation method overcomes the defects of a single strain, a low survival rate, poor stability, a short activity retention period, easy occurrence of autolysis, etc. of a variety of conventional microbial preparations and greatly improves the output and the preserving rate of microbial inocula; and as a feed additive for livestock, the prepared biological microbial protease preparation can prevent and resist diseases, improve the output of the livestock and increase the growth speed of the livestock.

The invention discloses an apozymase and a preparation method. The apozymase can be directly processed into oral liquid for preventing and treating hypertension and hyperlipidaemia. The apozymase is formed by adding sweet water to fruit raw materials and adding sweet water to alga raw materials for natural fermenting. The apozymase is characterized in that the fruit raw materials include apple, banana, orange, papaya, mango, pineapple, cherry, grape, peach, litchi, plum, pomegranate, strawberry, pear, watermelon and longan, and the alga raw materials include hair weeds, nori, kelp and sea lettuce. The abundant nutrients contained in the fruit raw materials are fermented into fruit raw material fermenting liquid containing abundant nutrients and microorganism protease, and the apozymase with a function of preventing and treating hypertension is obtained by sufficiently mixing and fermenting fruit raw material fermenting liquid and alga raw material fermenting liquid; the sufficient fusion of effective components in fruits and nutrients in alga raw material fermenting liquid is ensured, so the effective components and the nutrients are easily absorbed by human bodies.