13 results about "Microbial protease" patented technology

The present invention relates to a microbial protease for cell detachment. The present invention also relates to a composition suitable for cell detachment containing said microbial protease, the use of said microbial protease in a cell detachment process, and a method for cell detachment using said microbial protease.

The present invention provides a novel proteolytic enzyme composition more particularly an orally administered pro¬teolytic enzyme composition comprising of one or more acid proteases, one or more alkaline protease and one or more plant proteases. More particularly, the composition comprises of microbial (fungal, bacterial or other microbes) protease enzymes, proteases from plant and animals proteases thereof.

The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.

The invention relates to the technical field of meat product processing, in particular to a dried meat slice tenderizing process based on microorganism source protease, which comprises the following steps: selecting fresh raw material meat, removing fascia, cutting into slices, putting into a NaCl solution, and carrying out ultrasonic treatment to remove blood and fishy smell; performing high-voltage pulse electric field treatment in pulse electric field treatment equipment; adding a compound microbial enzyme preparation for enzymolysis treatment, wherein the compound microbial enzyme preparation is prepared by compounding bacillus subtilis neutral protease and aspergillus oryzae acid protease according to the ratio of (1: 0.9)-(1: 1.1); putting the raw material meat subjected to enzymolysis treatment and the marinade into a vacuum tumbling machine, and performing vacuum tumbling to obtain a meat material; spreading the meat on a screen mesh, and baking to obtain semi-finished dried meat slices; and cooling, cutting, detecting and packaging the semi-finished dried meat slices to obtain finished dried meat slices. According to the invention, the food safety is ensured, the taste and flavor of the dried meat slice are obviously enhanced, and the requirements of consumers on high-quality meat products are met.

The invention provides a method for accurately predicting a substrate protein hydrolysis site in a microbial fermentation process, and belongs to the technical field of molecular biology. The method comprises the following steps: screening protease secreted by target microorganisms based on whole genome data by using a bioinformatics means; establishing a three-dimensional structure model of the target microbial protease; performing molecular docking on the target microbial protease and the substrate protein to obtain an optimal docking conformation; the interaction relation between the target microorganism protease and the substrate protein under the fermentation condition is studied through molecular dynamics simulation; and analyzing molecular docking and molecular dynamics simulation results, and predicting the hydrolysis site of the substrate protein in the microbial fermentation process. According to the method, molecular docking and molecular dynamics simulation are utilized, the binding site of the enzyme and the substrate protein in the microbial fermentation process can be accurately predicted, meanwhile, the polypeptide generation condition of the fermented protein substrate is analyzed, and therefore the microbial protease of the specific polypeptide can be hydrolyzed in a targeted localization mode.

The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.

The present invention relates to microbial proteases for cell isolation. The invention also relates to a composition which contains the microbial protease and is suitable for cell separation, application of the microbial protease in a cell separation process, and a cell separation method adopting the microbial protease.

The present invention relates to microbial proteases for cell detachment. The present invention also relates to compositions suitable for cell detachment comprising said microbial proteases, use of said microbial proteases in cell detachment processes, and methods of cell detachment employing said microbial proteases.

This invention discloses a method for preparing and applying a microbial protease liquid fertilizer, comprising the following steps: S1. Mixing animal and plant protein raw materials with water, and subjecting them to physical-assisted cell wall disruption to obtain a pretreated protein slurry; S2. Inoculating with a composite seed liquid, and performing aerobic fermentation and enzymatic hydrolysis at 45-50℃ for 48-60 hours, maintaining the pH of the fermentation broth at 6.5-7.5 to obtain an enzymatic hydrolysate; S3. Cooling to 28-35℃, inoculating with a mixed probiotic agent, and performing anaerobic or facultative anaerobic fermentation for 48-72 hours to obtain a fermentation broth; S4. Adding trace element salts, and stirring the reaction at 40℃ to 45℃ for 1.5-2.5 hours to allow small molecule active peptides in the fermentation broth to undergo in-situ complexation with metal ions, followed by post-treatment to obtain the microbial protease liquid fertilizer; Through the synergistic effect of physical cell wall disruption and composite enzymatic hydrolysis, the conversion efficiency of recalcitrant waste protein raw materials such as feather meal is significantly improved.

The application provides a method for accurately predicting a hydrolysis site of a substrate protein in a microbial fermentation process, and belongs to the technical field of molecular biology. The method comprises the following steps: screening a protease secreted by a target microorganism based on whole genome data and using bioinformatics means; establishing a three-dimensional structure model of the protease secreted by the target microorganism; performing molecular docking of the protease secreted by the target microorganism and a substrate protein to obtain an optimal docking conformation; studying the interaction relationship between the protease secreted by the target microorganism and the substrate protein under fermentation conditions by using molecular dynamics simulation; and analyzing the molecular docking and molecular dynamics simulation results to predict the hydrolysis site of the substrate protein in the microbial fermentation process. The method can accurately predict the site of the enzyme and the substrate protein in the microbial fermentation process by using molecular docking and molecular dynamics simulation, and can analyze the polypeptide produced by the substrate protein after fermentation, so that the microbial protease hydrolyzing specific polypeptides can be targeted and positioned.

The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications.

The invention discloses a goat milk extract with anti-wrinkle and repairing effects and an enzymolysis biological preparation method, and particularly relates to the technical field of cosmetics, the goat milk extract is prepared by performing low-temperature vacuum de-odoring treatment and treating easily-sensitized protein by adopting plant protease and microbial protease, and the goat milk extract is free of goaty flavor and sensitization, and has no side effects. Nutritional ingredients and effects of goat milk are reserved; the preparation method comprises the steps of goat milk pretreatment, low-temperature vacuum de-odoring, enzymolysis, centrifugal separation and the like. The extract is applied to cosmetic compositions, contains various components such as goat milk extract, other functional skin care product additives, nicotinamide and the like, and can synergistically promote collagen synthesis, enhance epidermis barrier repair capability and realize anti-wrinkle and repair effects.

The invention relates to the technical field of collagen, and particularly discloses a preparation method of collagen peptide. The preparation method of the collagen peptide comprises the following steps: crushing fish scales, soaking the crushed fish scales in a sodium hydroxide solution, soaking the soaked fish scales in a citric acid solution, and drying the soaked fish scales for later use; carrying out enzymolysis on the fish scale powder by using microbial protease and a functional agent to obtain a proteolysis solution; centrifuging the protein enzymatic hydrolysate, taking supernate, and performing ultrafiltration and freeze drying to obtain the collagen peptide. Wherein the functional agent is prepared by grafting vinyl pyrrolidone to mannitol and then coating the mannitol with a chitosan solution, and a traditional Chinese medicine preparation which is prepared by taking astragalus membranaceus and ginkgo leaves as raw materials through two-step extraction is added in the preparation process of the microbial protease. The collagen peptide prepared by the method has high purity, high activity and high yield, the extraction efficiency is greatly improved, and the collagen peptide has a wide application prospect.