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Microbial wall breaking method of ganoderma lucidum spore powder

A Ganoderma lucidum spore powder and microbial technology, applied in the field of microbial fermentation of Ganoderma lucidum spores, can solve the problems of difficult removal of exogenous enzymes and high cost, and achieve the effect of facilitating absorption and utilization

Inactive Publication Date: 2018-03-09
丹阳西联生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the enzymatic hydrolysis method has the disadvantages of high cost and difficulty in removing exogenous enzymes after breaking the wall.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A microbial wall-breaking method of Ganoderma lucidum spore powder, the method comprises the following steps:

[0021] (1) Sterilization treatment of Ganoderma lucidum spore powder: the sterilization treatment of Ganoderma lucidum spore powder is carried out by microwave irradiation, the microwave frequency is 3000MHz, and the irradiation time is 2-3min;

[0022] (2) Preparation of microbial liquid: inoculate Streptomyces griseus in a liquid culture liquid, cultivate at 20° C. and 160 r / min for 3.5 d, and obtain a microbial liquid for subsequent use; the mass parts of the liquid culture liquid are composed as follows: 21 parts of soluble starch , 1.2 parts of potassium nitrate, 0.6 parts of magnesium sulfate, 0.6 parts of sodium chloride, 0.6 parts of dipotassium hydrogen phosphate, 0.02 parts of iron sulfate, 16 parts of agar, the pH value of the liquid culture solution is 7.5, and the liquid culture solution is in Sterilize at 122℃ for 30min, which is the best conditi...

Embodiment 2

[0025] A microbial wall-breaking method of Ganoderma lucidum spore powder, the method comprises the following steps:

[0026] (1) Sterilization treatment of Ganoderma lucidum spore powder: sterilize Ganoderma lucidum spore powder by ethylene oxide gas sterilization method in a sterilizer. The process conditions of ethylene oxide gas sterilization method are: inside the sterilizer The temperature of the sterilizer is 38~52℃, the sterilization time is 12~24h, the relative humidity in the sterilization cabinet is 35~65%, and the concentration of ethylene oxide gas is 900~1400mg / l.

[0027] (2) Preparation of microbial liquid: inoculate Streptomyces griseus in a liquid culture liquid, cultivate at 24° C. and 160 r / min for 3 d, and obtain a microbial liquid for subsequent use; the mass parts of the liquid culture liquid are composed as follows: 23 parts of soluble starch, 1.5 parts of potassium nitrate, 0.7 parts of magnesium sulfate, 0.7 parts of sodium chloride, 0.7 parts of dipo...

Embodiment 3

[0031] A microbial wall-breaking method of Ganoderma lucidum spore powder, the method comprises the following steps:

[0032] (1) Sterilization treatment of Ganoderma lucidum spore powder: the sterilization treatment of Ganoderma lucidum spore powder is carried out by microwave irradiation, the microwave frequency is 30000MHz, and the irradiation time is 2-3min;

[0033] (2) Preparation of microbial liquid: inoculate Streptomyces griseus in a liquid culture liquid, cultivate at 26° C. and 160 r / min for 2.5 d, and obtain a microbial liquid for subsequent use; the mass parts of the liquid culture liquid are composed as follows: 25 parts of soluble starch , 1.8 parts of potassium nitrate, 0.8 parts of magnesium sulfate, 0.8 parts of sodium chloride, 0.8 parts of dipotassium hydrogen phosphate, 0.05 parts of iron sulfate, 18 parts of agar, the pH value of the liquid culture solution is 7.5, and the liquid culture solution is in Sterilize at 125℃ for 25min, this condition is the be...

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PUM

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Abstract

The invention relates to the technical field of wall breakage of ganoderma lucidum spore powder, in particular to a method for performing wall breakage on ganoderma lucidum spore through microbial fermentation. The method comprises the following steps: (1) performing sterilizing treatment on the ganoderma lucidum spore powder; (2) preparing microbial liquid; and (3) uniformly mixing the ganodermalucidum spore powder sterilized in the step (1) and the microbial liquid in the step (2) according to the soil liquid mass ration of 1:(1.1-1.8), controlling the temperature to 20 to 25 DEG C, standing, performing lucifugal fermentation for 5 to 7 days, performing soil and liquid separation after the fermentation, and drying to obtain the wall-broken ganoderma lucidum spore powder. By adoption ofthe technical scheme of the invention, the method has the following beneficial effects: streptomyces griseus serves wall-dissolving bacteria, so that the ganoderma lucidum spore can be subjected to wall breakage effectively and safely, the active substances of the ganoderma lucidum spore powder are remained and protected to the greatest degree and absorption and utilization of a human body are benefited; and by the microbial fermentation process, the ganoderma lucidum spore can be subjected to wall breakage, and the generated microbial protease can synergistically enhance the effect of the ganoderma lucidum spore powder.

Description

technical field [0001] The invention relates to the technical field of Ganoderma lucidum spore powder wall breaking, in particular to a method for microbial fermentation of ganoderma lucidum spore wall breaking. Background technique [0002] Ganoderma lucidum spore powder is the seed of Ganoderma lucidum, the extremely tiny oval reproductive cells ejected from the gill gills of Ganoderma lucidum during the growth and maturity stage. Each Ganoderma lucidum spore is only 4-6 microns. It is a living organism with a double-walled structure and is surrounded by hard chitin cellulose, which is difficult for the human body to fully absorb. After the wall is broken, it is more suitable for direct absorption by the human stomach and intestines. It condenses the essence of Ganoderma lucidum, and has all the genetic material and health care effects of Ganoderma lucidum. On average, 1 kilogram of pure spore powder can be collected for every 100 kilograms of Ganoderma lucidum. Accordin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/06C12R1/645
Inventor 徐亚敏
Owner 丹阳西联生物技术有限公司
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