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Process for preparing dried soybean polypeptide powder with fat reducing action

A production process and technology of dry peptide powder, applied in the processing field of soybean protein isolate, can solve the problems of difficulty in obtaining the final product with lipid-lowering effect, loss of activity of hydrolyzate, inconsistent hydrolyzate, etc., and achieve shortened hydrolysis time, reduced energy consumption, Specificity-enhancing effect

Inactive Publication Date: 2004-11-03
武汉天天好生物制品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many technologies for enzymatically hydrolyzing soybean protein isolate. Some use a single type of enzyme to hydrolyze soybean protein isolate, which requires too long time and consumes energy and time. With the prolongation of hydrolysis time, The activity of the hydrolyzate is also gradually lost, and the lipid-lowering effect of the final product is poor
Some use multiple varieties of enzymes to hydrolyze soybean protein isolate at the same time, but choose different varieties of enzymes and their combinations, and use different hydrolysis conditions to obtain different hydrolysates, and the characteristics of the hydrolysates are also different. Different, and a little carelessness in the operation will cause the hydrolyzate to be inconsistent, and it is difficult to obtain the final product with significant lipid-lowering effect and stable performance

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: Get 15 kilograms of soybean protein isolates with a total nitrogen content greater than or equal to 11%, add 50 kilograms of distilled water, and use a colloid mill to grind it into a fine and uniform soybean protein isolate slurry. Then add 200 kilograms of distilled water, put it into the reaction tank, and adjust its pH value to 6.7 with a sodium hydroxide solution with a weight content of 20%. Then add 600 g of neutral microbial protease labeled AS1.398 under stirring, and heat to 48° C. for hydrolysis. During the hydrolysis process, 20% sodium hydroxide solution was used to continuously adjust and maintain the pH value in the range of 6.7-7.0, and the hydrolysis was continued for 8 hours. After the first hydrolysis is completed, adjust and maintain its pH value to 7.5 with 20% sodium hydroxide solution, keep its temperature in the range of 48-52°C, add 520 g of trypsin under constant stirring, and continue hydrolysis for 7 Hours. After the second hy...

Embodiment 2

[0018] Embodiment 2: Get 15 kilograms of soybean protein isolates with a total nitrogen content greater than or equal to 11%, add 65 kilograms of distilled water, and use a colloid mill to grind it into a fine and uniform soybean protein isolate slurry. Then add 400 kg of distilled water, put it into the reaction tank, and adjust its pH value to 7.0 with a 15% sodium hydroxide solution by weight. Then add 750 g of neutral microbial protease labeled AS1.398 under stirring state, and heat to 50° C. for hydrolysis. During the hydrolysis process, 15% sodium hydroxide solution was used to continuously adjust and maintain the pH value in the range of 6.7-7.0, and the hydrolysis was continued for 10 hours. After the first hydrolysis is completed, adjust and maintain its pH value to 8.0 with 15% sodium hydroxide solution, keep its temperature in the range of 48-52 ° C, add 750 g of trypsin under constant stirring, and continue hydrolysis for 9 Hours. After the second hydrolysis is c...

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Abstract

A dried soybean polypeptide powder with hypolipemic function is prepared through adding distilled water to separated sobyean protein, grinding, adding neutral microproteinase, regulating pH value and temp., hydrolyzing, adding pancreatin, regulating pH value and temp., hydrolyzing again, neutralizing, boiling for deactivating enzyme, cooling, filtering, vacuum concentrating and spray drying. Its advantages are high activity and excellent hypolipemic and antioxidizing functions.

Description

technical field [0001] The invention relates to the processing of soybean protein isolate, in particular to a production process from which soybean polypeptide dry powder with lipid-lowering effect can be obtained. Background technique [0002] As a low-fat, high-protein natural nutritious food, soybean protein isolate is very suitable for people with high blood lipids, and can also be used to prevent and treat hyperlipidemia. However, soy protein isolate is mostly composed of macromolecular proteins, which contain anti-nutritional factors such as trypsin inhibitors, which are not easy for the human body to digest and absorb. For this reason, it is necessary to change the macromolecular protein in soybean protein isolate into small molecular peptides that can be directly absorbed by the human body. The theory of biochemistry tells us that the conversion of macromolecular proteins into small molecular peptides can generally be hydrolyzed by acid-base...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K38/02A61P3/06C12P21/00
Inventor 陈栋梁殷腊生
Owner 武汉天天好生物制品有限公司
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