Preparation method of acidified small-peptide protein feed

A peptide protein and feed technology, applied in the field of enzyme preparations, microorganisms, and feed, can solve the problems of low content of anti-nutritional factors, unbalanced amino acid nutrition, unstable quality, etc., and achieve low content of anti-nutritional factors, balanced amino acids, and palatability Good results

Inactive Publication Date: 2014-07-23
FLSUGARPEPTIDE BIOLOGY ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The present invention aims to overcome the mold contamination of the above-mentioned fermented soybean meal, incomplete fermentation and enzymolysis, low content of small peptides and lactic acid, unstable quality, poor feeding effect, high drying cost, prone to Maillard reaction, and unbalanced amino acid nutrition To solve such problems, a preparation method of acidified small peptide protein feed is provided. The acidified small peptide protein feed prepared by this method is rich in small molecule nutrients such as small peptides, lactic acid, yeast protein and probiotics, and has amino acid balance, high nutrition, and easy digestion. , good palatability and low content of anti-nutritional factors

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Remove impurities and grind 80kg of soybean meal and 20kg of rice protein, mix them evenly, then add 1500kg of water to prepare a fermented enzymatic hydrolysis slurry, adjust the pH to 7.0 with 20% (mass percentage, the same below) calcium hydroxide solution, and feed steam to raise the temperature Sterilize at 121°C for 30 minutes, pass cooling circulating water to lower the temperature to 35°C, add 160kg of Bacillus subtilis seed liquid and 80kg of yeast seed liquid, carry out liquid aerobic fermentation, enzymatic hydrolysis for 48 hours, and pass through the plate 1520kg of small peptide protein liquid and 100kg of protein solids wet basis are separated by box separation or centrifuge. After three-effect concentration, 1520kg of small peptide protein liquid was concentrated to obtain 100kg of small peptide protein slurry.

[0028] After mixing 100kg of protein solids wet basis, 250kg of soybean meal, 30kg of lactic acid bacteria seed liquid and 0.5kg of microbial p...

Embodiment 2

[0034] 600kg of soybean meal, 200kg of rice protein, and 200kg of wheat protein were removed and pulverized, mixed evenly, and then 20,000kg of water was added to prepare a fermented enzymolysis slurry, and the pH was adjusted to 6.8 with 20% calcium hydroxide solution (mass percentage, the same below). Put in steam to heat up to 121°C for 30 minutes to sterilize, put in cooling circulating water to lower the temperature to 28°C, add 180kg of Bacillus subtilis seed liquid and 100kg of yeast seed liquid, carry out liquid aerobic fermentation and enzymatic hydrolysis for 48 hours Finally, 20,000kg of small peptide protein liquid and 800kg of protein solids wet basis are separated by plate and frame separation or centrifuge. After three-effect concentration, 20,000 kg of small peptide protein liquid was concentrated to obtain 800 kg of small peptide protein slurry.

[0035] After mixing 800kg of protein solids wet basis, 2000kg of soybean meal, 250kg of lactic acid bacteria seed ...

Embodiment 3

[0038] Remove impurities and pulverize 1000kg soybean meal, mix well, then add 18000kg water to prepare fermented enzymolysis slurry, adjust pH to 7.0 with 20% calcium hydroxide solution (mass percentage, the same below), and heat up to 121°C to extinguish Bacteria for 30 minutes, when the temperature is lowered to 32°C by passing through cooling circulating water, 150kg of Bacillus subtilis seed liquid and 50kg of yeast seed liquid are inserted, and liquid aerobic fermentation and enzymatic hydrolysis are carried out for 48 hours, then separated by plate frame or centrifuged Machine separated 18000kg small peptide protein liquid and 1200kg protein solids wet basis. After three-effect concentration, 18000kg of small peptide protein liquid was concentrated to obtain 900kg of small peptide protein slurry.

[0039] After mixing 1200kg of protein solids wet basis, 3000kg of soybean meal, 400kg of lactic acid bacteria seed liquid and 6kg of microbial protease, a second solid-state ...

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PUM

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Abstract

The invention belongs to the technical field of feeds, enzymic preparations and microorganisms, and in particular relates to a preparation method of an acidified small-peptide protein feed. The preparation method comprises the following steps: mixing soybean meal, rice protein, wheat protein, potato protein and water; performing size mixing, sterilizing and cooling so as to prepare a fermentation broth; adding bacillus subtilis strain liquid and saccharomycetes strain liquid to the fermentation broth to perform aerobic fermentation and enzymolysis on the liquids; carrying out solid-liquid separation to obtain small-peptide protein fluid and a protein solid content wet basis; concentrating small-peptide protein fluid to obtain small-peptide protein size; mixing the protein solid content wet basis, a protein feed, lactic acid bacteria strain liquid and microorganism protease; performing solid anaerobic fermentation and enzymolysis to obtain fermentation protein residue; absorbing and uniformly mixing the small-peptide protein size, the fermentation protein residue and lactic acid; and then drying. The acidified small-peptide protein feed is rich in small peptide, lactic acid, yeast protein, probiotics and other small molecular nutrients, and has the characteristics of being balanced in amino acid, high in nutrition, easy to digest, high in palatability, low in content of antinutritional factors, and the like.

Description

technical field [0001] The invention belongs to the technical fields of feed, enzyme preparations and microorganisms, and in particular relates to a preparation method of acidified small peptide protein feed. Background technique [0002] As we all know, my country's fishery resources are scarce, fishmeal production is insufficient, and the quality is deviation. my country's per capita milk output is very low, the scale of the dairy product deep processing industry is small, and all whey powder for feeding is imported. As far as high-grade feed raw materials are concerned, my country uses foreign exchange to import more than 1 million tons of fish meal and more than 300,000 tons of whey protein powder every year. In 2012, China imported 1.17 million tons of fishmeal and 380,000 tons of whey protein powder. In addition, there are certain safety hazards in animal-derived products. Because of this, the use of animal-derived feed products such as fish meal and blood meal is p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/14A23K10/12A23K20/147A23K40/00A23K50/30A23K50/60
Inventor 程茂基程勐万里孟龙薛芹吕锋
Owner FLSUGARPEPTIDE BIOLOGY ENG
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