Preparation method of acidified small-peptide protein feed
A peptide protein and feed technology, applied in the field of enzyme preparations, microorganisms, and feed, can solve the problems of low content of anti-nutritional factors, unbalanced amino acid nutrition, unstable quality, etc., and achieve low content of anti-nutritional factors, balanced amino acids, and palatability Good results
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Embodiment 1
[0027] Remove impurities and grind 80kg of soybean meal and 20kg of rice protein, mix them evenly, then add 1500kg of water to prepare a fermented enzymatic hydrolysis slurry, adjust the pH to 7.0 with 20% (mass percentage, the same below) calcium hydroxide solution, and feed steam to raise the temperature Sterilize at 121°C for 30 minutes, pass cooling circulating water to lower the temperature to 35°C, add 160kg of Bacillus subtilis seed liquid and 80kg of yeast seed liquid, carry out liquid aerobic fermentation, enzymatic hydrolysis for 48 hours, and pass through the plate 1520kg of small peptide protein liquid and 100kg of protein solids wet basis are separated by box separation or centrifuge. After three-effect concentration, 1520kg of small peptide protein liquid was concentrated to obtain 100kg of small peptide protein slurry.
[0028] After mixing 100kg of protein solids wet basis, 250kg of soybean meal, 30kg of lactic acid bacteria seed liquid and 0.5kg of microbial p...
Embodiment 2
[0034] 600kg of soybean meal, 200kg of rice protein, and 200kg of wheat protein were removed and pulverized, mixed evenly, and then 20,000kg of water was added to prepare a fermented enzymolysis slurry, and the pH was adjusted to 6.8 with 20% calcium hydroxide solution (mass percentage, the same below). Put in steam to heat up to 121°C for 30 minutes to sterilize, put in cooling circulating water to lower the temperature to 28°C, add 180kg of Bacillus subtilis seed liquid and 100kg of yeast seed liquid, carry out liquid aerobic fermentation and enzymatic hydrolysis for 48 hours Finally, 20,000kg of small peptide protein liquid and 800kg of protein solids wet basis are separated by plate and frame separation or centrifuge. After three-effect concentration, 20,000 kg of small peptide protein liquid was concentrated to obtain 800 kg of small peptide protein slurry.
[0035] After mixing 800kg of protein solids wet basis, 2000kg of soybean meal, 250kg of lactic acid bacteria seed ...
Embodiment 3
[0038] Remove impurities and pulverize 1000kg soybean meal, mix well, then add 18000kg water to prepare fermented enzymolysis slurry, adjust pH to 7.0 with 20% calcium hydroxide solution (mass percentage, the same below), and heat up to 121°C to extinguish Bacteria for 30 minutes, when the temperature is lowered to 32°C by passing through cooling circulating water, 150kg of Bacillus subtilis seed liquid and 50kg of yeast seed liquid are inserted, and liquid aerobic fermentation and enzymatic hydrolysis are carried out for 48 hours, then separated by plate frame or centrifuged Machine separated 18000kg small peptide protein liquid and 1200kg protein solids wet basis. After three-effect concentration, 18000kg of small peptide protein liquid was concentrated to obtain 900kg of small peptide protein slurry.
[0039] After mixing 1200kg of protein solids wet basis, 3000kg of soybean meal, 400kg of lactic acid bacteria seed liquid and 6kg of microbial protease, a second solid-state ...
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