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Novel method for preparing Acaudina leucoprocta collagen peptide through hydrolyzing microorganism protease

A technology of microbial protease and black ginseng collagen, which is applied in the field of preparation of collagen peptides, can solve problems such as difficult control of the process, waste of nutrients, incomplete hydrolysis, etc., and achieve the effects of improving absorption utilization, promoting absorption, and increasing affinity

Inactive Publication Date: 2018-05-11
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many methods for the preparation of collagen peptides. The existing technology, such as the Chinese invention patent with the authorized public number CN102251006 B, discloses a method for producing collagen from black ginseng. , complex protease, and flavor protease are directly hydrolyzed, and then membrane filtration, concentration, and drying are used to prepare the collagen product of Donghai black ginseng. It has the characteristics of a simple preparation method, but the process of this method is not easy to control. It is easy to hydrolyze incompletely, waste nutrients, and too much protease is added in this preparation method, because the enzymatic hydrolysis reaction rate has K M value, not by the amount of enzyme, thus causing waste of enzyme

Method used

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  • Novel method for preparing Acaudina leucoprocta collagen peptide through hydrolyzing microorganism protease

Examples

Experimental program
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Effect test

Embodiment 1

[0022] The hydrolysis of novel microbial proteases to prepare collagen peptides from black ginseng includes the following steps:

[0023] 1) Put sea cucumber body wall homogenate in 0.05mol / L acetic acid solution system, add 0.3% of substrate weight pepsin for restriction enzymolysis, control the temperature at 5°C, and double-frequency ultrasonic-assisted enzymolysis, the ultrasonic frequencies are respectively 30kHz and 60kHz, continue to stir at low speed for 48 hours, inactivate the enzyme in a boiling water bath, centrifuge the enzymolysis solution at 10,000r / min at high speed, take the supernatant, precipitate and repeat the enzymolysis operation twice, collect the supernatant, freeze-dry to obtain Donghai black ginseng collagen dry product;

[0024] 2) Weigh 35g of glycerin, 30g of soybean meal powder, 10g of bran, 5.0g of urea, Na 2 HP04 4g,K 2 HP0 4 0.3g / L, MgSO 4 1.44g, CaCl 2 1.1g, NaCl5.0g and 910 parts of distilled water, pH natural, 115 ℃ autoclaving for 20 ...

Embodiment 2

[0029] The hydrolysis of novel microbial proteases to prepare collagen peptides from black ginseng includes the following steps:

[0030] 1) Pretreatment: Take the fresh and clean body wall of black ginseng, soak it in 2% hydrochloric acid solution and 0.05% calcium hydroxide solution for 48 hours, stir intermittently during the process, change the solution every 18 hours, and use deionized water after soaking Rinse with water until neutral; soak the body wall of black cucumber in 6% sodium hydroxide solution until the body wall of sea cucumber becomes transparent, remove it, rinse with deionized water until neutral, then chop and homogenate; pretreatment can remove Calcium and non-collagen substances in the body wall of sea cucumbers for further extraction of collagen;

[0031] 2) Extraction: put sea cucumber body wall homogenate in 0.08mol / L acetic acid solution system, add 0.5% of substrate weight pepsin for restricted enzymolysis, control the temperature at 10°C, and enzym...

Embodiment 3

[0037] The technical solution adopted is: hydrolysis of new microbial proteases to prepare collagen peptides from black ginseng of the East China Sea, including: pretreatment, extraction, fermentation, purification, hydrolysis, and purification, specifically including the following steps:

[0038] Pretreatment: take fresh and clean body wall of black ginseng from the East China Sea, soak it in 2% hydrochloric acid solution for 45 hours, stir intermittently, change the solution every 15 hours, wash it with deionized water until neutral after soaking; then wash it with 0.04% calcium hydroxide Soak in the solution for 45 hours, stir intermittently, change the solution once every 15 hours, rinse with deionized water until neutral after soaking; soak the body wall of black cucumber in 6% sodium hydroxide solution until the body wall of sea cucumber is transparent and remove , washed with deionized water until neutral, then chopped and homogenized; pretreatment can remove calcium and...

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Abstract

The invention discloses a novel method for preparing an Acaudina leucoprocta collagen peptide through hydrolyzing a microorganism protease. The method comprises the steps of pretreatment, extraction,fermentation, purification, hydrolysis and purification. The method has the following advantages: addition of 2,3-dimethyl-2-butanol and erythrose in the enzymatic hydrolysis process cooperates with bifrequency ultrasonic treatment can reduce the energy barrier between a ground state and an active state in order to reduce the Ea value and the KM value, so the matrix and enzyme affinity is increased, and the enzymatic reaction rate is improved; and the Bacillus spp protease having a high enzymatic hydrolysis vitality can destroy the unique triplex super helical structure of an Acaudina leucoprocta collagen, and hydrolyzes the Acaudina leucoprocta collagen into polypeptides, small peptides, amino acids and other micro-molecular substances having small relative molecular weights, so the absorption and utilization rate is greatly improved after the hydrolysis, and the absorption of other proteins in foods can be promoted. The collagen peptide has multiple medical healthcare effects, and isa nutritional healthcare substance being easy to absorb and having no toxic or side effects.

Description

technical field [0001] The invention relates to the field of preparation of collagen peptides, in particular to preparation of Donghai black ginseng collagen peptides through hydrolysis of novel microbial proteases. technical background [0002] Donghai black ginseng (scientific name: Acaudina leucoprocta) is one of more than 20 kinds of edible sea cucumbers in China. It is rich in the East China Sea area. It is widely distributed in our province, especially in the coastal areas of Ningbo and Zhoushan. The fishing volume reaches tens of thousands of tons. Donghai black cucumber has extremely high nutritional value and medicinal value. It is rich in protein, sea cucumber saponin, sea cucumber polysaccharide, collagen, vitamins, essential fatty acids, and major and trace elements. It does not contain cholesterol. Long-term consumption can enhance physical fitness, Prevent diseases, inhibit tumors, and delay aging. However, for a long time, due to the lack of key technologies...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
CPCC12P21/06
Inventor 金火喜徐红萍杨立业欧阳小琨
Owner ZHEJIANG OCEAN UNIV
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