Leather softening compound enzyme with acid proteases and method for preparing leather softening compound enzyme

An acid protease and leather softening technology, which is applied in the field of leather softening compound enzyme containing acid protease and its preparation, can solve the problems of unsatisfactory leather softening effect, low antioxidant capacity, large loss of enzyme activity, etc., so as to ensure food safety. , the effect of improving the utilization rate of raw materials

Inactive Publication Date: 2016-04-27
湖南新鸿鹰生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem solved by the present invention is to overcome the defects of low acid protease activity, incomplete complex enzyme enzyme system, low antioxidant capacity, large enzyme activity loss and unsatisfactory leather softening effect in the existing leather softening compound enzyme, so as to enrich the plant protease High-activity acid protease and microbial protease as the main raw materia

Method used

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  • Leather softening compound enzyme with acid proteases and method for preparing leather softening compound enzyme
  • Leather softening compound enzyme with acid proteases and method for preparing leather softening compound enzyme

Examples

Experimental program
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Embodiment 1

[0067] Embodiment 1 raw material preparation

[0068] 1. Preparation of plant extracts: uniformly mix barley malt and wheat malt in a mass ratio of 6:4, and grind them to a particle size of 0.8mm to obtain crushed malt; then put papaya, pineapple, and figs in 0.4% sodium bicarbonate The solution was ultrasonically cleaned in an ultrasonic cleaning machine with a power of 200W and a frequency of 30KHz for 8min, drained, crushed to a particle size of 0.8mm at room temperature, and uniformly mixed according to a mass ratio of 6:3:2, and crushed malt with 4 times the mass of the mixture was added to obtain For the raw material mixture, add water twice the mass of the raw material mixture, adjust the pH value to 3.5 with citric acid, and carry out microwave extraction under the conditions of power 200W and frequency 2000Hz, wherein, the total time of each microwave irradiation is 70s, and the interval irradiation is carried out: Irradiate for 10s with an interval of 10s, control th...

Embodiment 2

[0092] A leather softening complex enzyme containing acid protease, which consists of the following raw materials in parts by weight:

[0093] 30 parts of plant extract, 15 parts of acid protease, 8 parts of mold culture, 5 parts of acid lipase, 4 parts of nonionic surfactant, 2 parts of protective agent, 2 parts of activator, 0.4 part of antioxidant;

[0094] The mass components of the nonionic surfactant are: 30 parts of alkyl polyglycosides (APG), 25 parts of alkyl glucamides (AGA), 12 parts of N-dodecyl ethylenediamine triacetate (ED3A) , isomerized alcohol ether carboxylate AEC-110712 parts, lauramide ether carboxylate (LAEC) 9 parts, Pingpingjia c-1254 parts, JFC 4 parts;

[0095] The activator is formed by uniformly mixing inorganic salts with the following mass components: 12 parts of zinc chloride, 12 parts of sodium sulfate, 8 parts of magnesium chloride, and 4 parts of calcium chloride;

[0096] Described protecting agent is made up of the raw material of following...

Embodiment 3

[0102] A leather softening complex enzyme containing acid protease, which consists of the following raw materials in parts by weight:

[0103] 20 parts of plant extract, 10 parts of acid protease, 5 parts of mold culture, 3 parts of acid lipase, 2 parts of nonionic surfactant, 1 part of protective agent, 1 part of activator, 0.3 part of antioxidant;

[0104] The mass components of the nonionic surfactant are: 20 parts of alkyl polyglycosides (APG), 20 parts of alkyl glucamides (AGA), 10 parts of N-dodecyl ethylenediamine triacetate (ED3A) , isomerized alcohol ether carboxylate AEC-110710 parts, lauramide ether carboxylate (LAEC) 8 parts, Pingpingjia c-1253 parts, JFC 3 parts;

[0105] The activator is formed by uniformly mixing inorganic salts with the following mass components: 10 parts of zinc chloride, 10 parts of sodium sulfate, 5 parts of magnesium chloride, and 3 parts of calcium chloride;

[0106] Described protective agent is made up of the raw material of following p...

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Abstract

The invention discloses a leather softening compound enzyme with acid proteases and a method for preparing the leather softening compound enzyme. The leather softening compound enzyme and the method have the advantages that the leather softening compound enzyme is mainly made of the high-activity acid proteases with abundant plant proteases and microorganism proteases, mould culture, acid lipase, non-ionic surfactants, plant extract, antioxidants, protective agents, activators and the like are scientifically compounded, accordingly, the compound enzyme is provided with complete enzyme systems, is stable in activity and high in efficacy, permeability and degreasing capacity and can act mildly and moderately, the hand feel and physical and mechanical properties of crust leather can be obviously improved, the bare weight of the crust leather can be obviously increased, and the shrinkage temperatures of the crust leather of raw hide of wet-blue leather of different animals can be increased by 9-14 DEG C; the porosity of the crust leather can be improved by 7.85-10.96%; the breathability of the crust leather can be improved by 41.0-50.1 mL/cm<2>hr; the tensile strength of the crust leather can be improved by 6.51-7.47 N/mm<2>; the tear strength of the crust leather can be improved by 5.28-20 N/mm; the elongation of the crust leather can be improved by 4.8-8.7%; the quality of products can be greatly improved, and the production cost can be reduced.

Description

technical field [0001] The invention relates to a leather softening enzyme, in particular to a leather softening compound enzyme containing acid protease and a preparation method thereof. Background technique [0002] Enzymes are biological catalysts produced by biological cells and proteins with special catalytic capabilities. Enzymes can be extracted from animal tissues and microorganisms. Due to limited animal and plant raw materials, industrial enzymes mainly come from microorganisms. Microbial proteases for tanning are roughly divided into three categories, namely alkaline proteases, neutral proteases and acidic proteases. At present, alkaline and neutral proteases have been widely used in the dehairing and softening processes in tanning production. Some enzyme preparations or auxiliary agents for immersion, liming, degreasing and other processes have also been successfully developed in some countries, and have achieved relatively good results. Good results. However...

Claims

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Application Information

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IPC IPC(8): C12N9/50C12N9/20C14C1/08
Inventor 李洪兵李海清张锦杰朱永明胡永明李贵骏
Owner 湖南新鸿鹰生物工程有限公司
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